Construct: ORF TRCN0000488178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020451.1_s317c1
- DNA Barcode:
- TTTGGGGCAGTGGTGCCCTACGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FGFR2 (2263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488178
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_022970.3 | 99.9% | 100% | 696A>G |
2 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_006717708.3 | 97.4% | 97.5% | 1_57del;753A>G;1341_1342insGTAACA |
3 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_000141.4 | 96.7% | 96.7% | (many diffs) |
4 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001320658.2 | 96.5% | 96.4% | (many diffs) |
5 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144913.1 | 93.4% | 93.4% | 696A>G;2304_2305insGA;2307_2307delCins158 |
6 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_006717710.4 | 93.4% | 93.3% | (many diffs) |
7 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015920.2 | 93.1% | 93.1% | (many diffs) |
8 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015921.2 | 90% | 89.9% | (many diffs) |
9 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447890.1 | 87.1% | 87.1% | 1_57del;164_165ins267;486A>G |
10 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447888.1 | 86.8% | 86.9% | (many diffs) |
11 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144914.1 | 86.2% | 86.2% | 696A>G;747_748ins339 |
12 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_023029.2 | 86% | 85.9% | (many diffs) |
13 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144917.2 | 85.7% | 85.7% | 696A>G;939_940ins351 |
14 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447887.1 | 84% | 83.9% | (many diffs) |
15 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447891.1 | 84% | 83.9% | 1_57del;166_167ins345;408A>G |
16 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447889.1 | 83.8% | 83.7% | (many diffs) |
17 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144916.1 | 82.8% | 82.6% | (many diffs) |
18 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144919.2 | 82.6% | 82.6% | (many diffs) |
19 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144918.2 | 82.6% | 82.4% | (many diffs) |
20 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144915.1 | 81.2% | 79.9% | (many diffs) |
21 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015924.2 | 81% | 80.7% | (many diffs) |
22 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015925.2 | 76.5% | 76.1% | (many diffs) |
23 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001320654.1 | 68.3% | 66.2% | (many diffs) |
24 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447892.1 | 52.4% | 52.4% | 0_1ins1173 |
25 | human | 2263 | FGFR2 | fibroblast growth factor re... | NR_073009.2 | 51.1% | (many diffs) | |
26 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507349.2 | 89.5% | 95.2% | (many diffs) |
27 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241673.2 | 89.5% | 95.2% | (many diffs) |
28 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321975.1 | 89.5% | 95.2% | (many diffs) |
29 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507350.3 | 89.2% | 95% | (many diffs) |
30 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_010207.2 | 86.6% | 92.1% | (many diffs) |
31 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241674.2 | 86.4% | 91.9% | (many diffs) |
32 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507352.2 | 85.7% | 90.9% | (many diffs) |
33 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321976.1 | 85.4% | 90.7% | (many diffs) |
34 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321977.1 | 82.7% | 87.6% | (many diffs) |
35 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507354.3 | 79.6% | 85.3% | (many diffs) |
36 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241676.2 | 79.4% | 85.1% | (many diffs) |
37 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_201601.2 | 77% | 82.8% | (many diffs) |
38 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241675.2 | 76.9% | 82.3% | (many diffs) |
39 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241677.2 | 76.8% | 82.6% | (many diffs) |
40 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507357.3 | 74.3% | 79.8% | (many diffs) |
41 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_001347638.1 | 74.1% | 79.5% | (many diffs) |
42 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XR_001785477.1 | 33.7% | (many diffs) | |
43 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XR_001785478.1 | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2538
- ORF length:
- 2466
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcagc tggggtcgtt tcatctgcct ggtcgtggtc accatggcaa 121 ccttgtccct ggcccggccc tccttcagtt tagttgagga taccacatta gagccagaag 181 agccaccaac caaataccaa atctctcaac cagaagtgta cgtggctgcg ccaggggagt 241 cgctagaggt gcgctgcctg ttgaaagatg ccgccgtgat cagttggact aaggatgggg 301 tgcacttggg gcccaacaat aggacagtgc ttattgggga gtacttgcag ataaagggcg 361 ccacgcctag agactccggc ctctatgctt gtactgccag taggactgta gacagtgaaa 421 cttggtactt catggtgaat gtcacagatg ccatctcatc cggagatgat gaggatgaca 481 ccgatggtgc ggaagatttt gtcagtgaga acagtaacaa caagagagca ccatactgga 541 ccaacacaga aaagatggaa aagcggctcc atgctgtgcc tgcggccaac actgtcaagt 601 ttcgctgccc agccgggggg aacccaatgc caaccatgcg gtggctgaaa aacgggaagg 661 agtttaagca ggagcatcgc attggaggct acaaggtacg aaaccagcac tggagcctca 721 ttatggaaag tgtggtccca tctgacaagg gaaattatac ctgtgtggtg gagaatgaat 781 acgggtccat caatcacacg taccacctgg atgttgtgga gcgatcgcct caccggccca 841 tcctccaagc cggactgccg gcaaatgcct ccacagtggt cggaggagac gtagagtttg 901 tctgcaaggt ttacagtgat gcccagcccc acatccagtg gatcaagcac gtggaaaaga 961 acggcagtaa atacgggccc gacgggctgc cctacctcaa ggttctcaag cactcgggga 1021 taaatagttc caatgcagaa gtgctggctc tgttcaatgt gaccgaggcg gatgctgggg 1081 aatatatatg taaggtctcc aattatatag ggcaggccaa ccagtctgcc tggctcactg 1141 tcctgccaaa acagcaagcg cctggaagag aaaaggagat tacagcttcc ccagactacc 1201 tggagatagc catttactgc ataggggtct tcttaatcgc ctgtatggtg gtaacagtca 1261 tcctgtgccg aatgaagaac acgaccaaga agccagactt cagcagccag ccggctgtgc 1321 acaagctgac caaacgtatc cccctgcgga gacaggtaac agtttcggct gagtccagct 1381 cctccatgaa ctccaacacc ccgctggtga ggataacaac acgcctctct tcaacggcag 1441 acacccccat gctggcaggg gtctccgagt atgaacttcc agaggaccca aaatgggagt 1501 ttccaagaga taagctgaca ctgggcaagc ccctgggaga aggttgcttt gggcaagtgg 1561 tcatggcgga agcagtggga attgacaaag acaagcccaa ggaggcggtc accgtggccg 1621 tgaagatgtt gaaagatgat gccacagaga aagacctttc tgatctggtg tcagagatgg 1681 agatgatgaa gatgattggg aaacacaaga atatcataaa tcttcttgga gcctgcacac 1741 aggatgggcc tctctatgtc atagttgagt atgcctctaa aggcaacctc cgagaatacc 1801 tccgagcccg gaggccaccc gggatggagt actcctatga cattaaccgt gttcctgagg 1861 agcagatgac cttcaaggac ttggtgtcat gcacctacca gctggccaga ggcatggagt 1921 acttggcttc ccaaaaatgt attcatcgag atttagcagc cagaaatgtt ttggtaacag 1981 aaaacaatgt gatgaaaata gcagactttg gactcgccag agatatcaac aatatagact 2041 attacaaaaa gaccaccaat gggcggcttc cagtcaagtg gatggctcca gaagccctgt 2101 ttgatagagt atacactcat cagagtgatg tctggtcctt cggggtgtta atgtgggaga 2161 tcttcacttt agggggctcg ccctacccag ggattcccgt ggaggaactt tttaagctgc 2221 tgaaggaagg acacagaatg gataagccag ccaactgcac caacgaactg tacatgatga 2281 tgagggactg ttggcatgca gtgcccTCCC AGAGACCAAC GTTCAAGCAG TTGGTAGAAG 2341 ACTTGGATCG AATTCTCACT CTCACAACCA ATGAGGAATA CTTGGACCTC AGCCAACCTC 2401 TCGAACAGTA TTCACCTAGT TACCCTGACA CAAGAAGTTC TTGTTCTTCA GGAGATGATT 2461 CTGTTTTTTC TCCAGACCCC ATGCCTTACG AACCATGCCT TCCTCAGTAT CCACACATAA 2521 ACGGCAGTGT TAAAACATAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2581 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2641 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTG GGGCAGTGGT 2701 GCCCTACGGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt