Construct: ORF TRCN0000488190

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021472.1_s317c1
DNA Barcode:
CTGAACTGGGGGTAGCATCTTATC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PHLDB1 (23187)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488190
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23187 PHLDB1 pleckstrin homology like do... NM_001144759.3 99.8% 100% 2595A>G;3957_3958insTAAAC
2 human 23187 PHLDB1 pleckstrin homology like do... XM_005271474.5 98.2% 98.3% 1_66del;2661A>G;4023_4024insTAAAC
3 human 23187 PHLDB1 pleckstrin homology like do... XM_005271473.3 95.7% 95.8% (many diffs)
4 human 23187 PHLDB1 pleckstrin homology like do... NM_001144758.3 95.6% 95.7% (many diffs)
5 human 23187 PHLDB1 pleckstrin homology like do... NM_015157.4 95.6% 95.7% (many diffs)
6 human 23187 PHLDB1 pleckstrin homology like do... XM_005271472.3 94.6% 94.8% (many diffs)
7 human 23187 PHLDB1 pleckstrin homology like do... XM_006718803.3 93.5% 93.6% (many diffs)
8 human 23187 PHLDB1 pleckstrin homology like do... XM_005271471.3 93.2% 93.4% (many diffs)
9 human 23187 PHLDB1 pleckstrin homology like do... XM_006718802.3 92.8% 92.9% (many diffs)
10 human 23187 PHLDB1 pleckstrin homology like do... XM_005271470.3 92.5% 92.6% (many diffs)
11 human 23187 PHLDB1 pleckstrin homology like do... XM_005271469.3 92.3% 92.4% (many diffs)
12 human 23187 PHLDB1 pleckstrin homology like do... XM_006718801.3 91.8% 91.9% (many diffs)
13 human 23187 PHLDB1 pleckstrin homology like do... XM_005271468.3 91.5% 91.7% (many diffs)
14 human 23187 PHLDB1 pleckstrin homology like do... XM_006718800.3 90.5% 90.6% (many diffs)
15 human 23187 PHLDB1 pleckstrin homology like do... XM_006718799.3 89.8% 89.9% (many diffs)
16 human 23187 PHLDB1 pleckstrin homology like do... XM_006718798.3 88.9% 89% (many diffs)
17 human 23187 PHLDB1 pleckstrin homology like do... XM_017017410.1 88.1% 88.2% (many diffs)
18 human 23187 PHLDB1 pleckstrin homology like do... XM_017017409.1 87.6% 87.7% (many diffs)
19 human 23187 PHLDB1 pleckstrin homology like do... XM_017017408.1 87.1% 87.2% (many diffs)
20 human 23187 PHLDB1 pleckstrin homology like do... XM_011542710.2 86.1% 86.2% (many diffs)
21 human 23187 PHLDB1 pleckstrin homology like do... XM_006718797.3 85.6% 85.7% (many diffs)
22 human 23187 PHLDB1 pleckstrin homology like do... XM_011542708.3 85.5% 85.6% (many diffs)
23 human 23187 PHLDB1 pleckstrin homology like do... XM_011542709.2 85.5% 85.6% (many diffs)
24 human 23187 PHLDB1 pleckstrin homology like do... XM_006718796.4 85.4% 85.5% (many diffs)
25 human 23187 PHLDB1 pleckstrin homology like do... XM_011542707.2 85.3% 85.4% (many diffs)
26 human 23187 PHLDB1 pleckstrin homology like do... XM_017017407.1 85.3% 85.4% (many diffs)
27 human 23187 PHLDB1 pleckstrin homology like do... XM_011542706.2 84.4% 84.5% (many diffs)
28 human 23187 PHLDB1 pleckstrin homology like do... XM_011542705.2 83.8% 83.9% (many diffs)
29 human 23187 PHLDB1 pleckstrin homology like do... XM_011542704.2 83.6% 83.7% (many diffs)
30 human 23187 PHLDB1 pleckstrin homology like do... XM_011542703.2 83% 83.1% (many diffs)
31 human 23187 PHLDB1 pleckstrin homology like do... XM_005277680.3 82.9% 83.1% (many diffs)
32 human 23187 PHLDB1 pleckstrin homology like do... XM_011542711.2 77.7% 77.8% (many diffs)
33 human 23187 PHLDB1 pleckstrin homology like do... XM_011542713.2 77.6% 77.7% (many diffs)
34 human 23187 PHLDB1 pleckstrin homology like do... XM_011542714.2 58.5% 58.3% (many diffs)
35 human 23187 PHLDB1 pleckstrin homology like do... XM_005271475.3 58.2% 57.5% (many diffs)
36 human 23187 PHLDB1 pleckstrin homology like do... XM_017017411.1 58.2% 57.5% (many diffs)
37 human 23187 PHLDB1 pleckstrin homology like do... XR_947813.2 57.6% (many diffs)
38 human 23187 PHLDB1 pleckstrin homology like do... XR_001747798.1 57.5% (many diffs)
39 human 23187 PHLDB1 pleckstrin homology like do... XR_001747797.1 57.3% (many diffs)
40 human 23187 PHLDB1 pleckstrin homology like do... XM_011542715.3 54.3% 53.9% (many diffs)
41 human 23187 PHLDB1 pleckstrin homology like do... XR_001747799.1 53.4% (many diffs)
42 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243978.1 87.4% 94.1% (many diffs)
43 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243976.1 86% 92.6% (many diffs)
44 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243975.1 85% 91.6% (many diffs)
45 mouse 102693 Phldb1 pleckstrin homology like do... NM_153537.4 84.4% 90.9% (many diffs)
46 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243974.1 83.7% 90.2% (many diffs)
47 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243973.1 83.3% 89.7% (many diffs)
48 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243972.1 82.4% 88.8% (many diffs)
49 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243971.1 82.2% 88.5% (many diffs)
50 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243970.1 81.8% 88.1% (many diffs)
51 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243969.1 80.6% 86.8% (many diffs)
52 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243968.1 79.8% 86% (many diffs)
53 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243967.1 79.7% 85.9% (many diffs)
54 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243983.1 79.3% 85.6% (many diffs)
55 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243966.1 79% 85.1% (many diffs)
56 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243965.1 78.1% 84.1% (many diffs)
57 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243964.1 77.9% 83.9% (many diffs)
58 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243963.1 77.2% 83.1% (many diffs)
59 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243962.1 76.8% 82.7% (many diffs)
60 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243961.1 76.5% 82.4% (many diffs)
61 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243960.1 76.2% 82.1% (many diffs)
62 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243982.1 76% 82% (many diffs)
63 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243957.1 75.7% 81.5% (many diffs)
64 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243958.1 75.7% 81.5% (many diffs)
65 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243956.1 75.5% 81.3% (many diffs)
66 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243955.1 75.1% 80.9% (many diffs)
67 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243953.1 74.9% 80.7% (many diffs)
68 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243954.1 74.9% 80.7% (many diffs)
69 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243981.1 74.6% 80.5% (many diffs)
70 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509907.3 74.4% 80.1% (many diffs)
71 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509916.3 74.4% 80.1% (many diffs)
72 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509919.4 74.4% 80.1% (many diffs)
73 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243980.1 73.7% 79.5% (many diffs)
74 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243959.1 73.1% 77.9% (many diffs)
75 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243979.1 69.7% 75.2% (many diffs)
76 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509928.4 67.5% 72.9% (many diffs)
77 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243977.1 67.5% 72.9% (many diffs)
78 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243984.1 56.1% 57.3% (many diffs)
79 mouse 102693 Phldb1 pleckstrin homology like do... XM_030243985.1 55.8% 56.3% (many diffs)
80 mouse 102693 Phldb1 pleckstrin homology like do... XM_006509933.3 41.2% 44.8% (many diffs)
81 mouse 102693 Phldb1 pleckstrin homology like do... XM_017313091.2 36.2% 39.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
4023
ORF length:
3957
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat
61tcaccatgga cgctctcaat aggaaccaaa taggccctgg atgccagacc cagaccatgg
121tgcagaaagg acccttggac ctgatcgaga caggcaaagg gctgaaagtg caaacggaca
181aaccccacct ggtgagcctg ggcagtgggc gactcagcac agccatcacc ctcctgccgc
241tggaggaagg gaggacggtg attggctctg cagccagaga catctcacta cagggcccag
301gcctggctcc agagcactgc tacatcgaga acctgcgggg caccctcacc ctctacccct
361gtggcaatgc ctgcactatt gatgggctcc ctgtccggca gcctacccgg ctcactcagg
421gctgcatgtt gtgcctgggt cagtccacct tccttcgctt taaccacccg gctgaagcca
481agtggatgaa aagcatgatt ccagcagggg gccgagcccc tgggcccccc tacagccctg
541ttcctgcaga atcagaaagt ctggtaaatg ggaaccacac cccacagact gcaacacggg
601gaccctctgc ctgtgccagc cacagttccc tggtgagctc tattgagaag gacctgcaag
661agatcatgga ctcactggtg ctagaggagc ctggagctgc tggcaagaag cctgccgcaa
721cctctccact gtcaccgatg gctaatggtg ggcgctacct gctgtctccc ccaaccagcc
781ccggcgccat gtctgtgggc tccagctatg agaacacctc tccagccttc tctccactct
841cttcaccagc cagcagtgga agctgtgcca gtcactcacc cagtgggcaa gagccaggac
901cttctgtgcc cccgctggta cctgcccgtt cctccagcta ccatctggcc ctacagcccc
961cacagtcccg cccaagtggt gctcgctccg agagtcctcg gctgagcagg aaagggggcc
1021atgagaggcc tcccagccct ggcctccggg gtctgctgac agacagccct gcagctactg
1081tcttggcgga ggcccggaga gccactgaga gcccccggct gggtgggcag ctgcctgtgg
1141tggccatcag cctgagtgaa tacccagctt ctggtgctct cagtcaaccc accagcattc
1201ctggcagccc caagtttcag cctccagtcc ctgctccccg aaacaagatt ggcacactcc
1261aggaccgccc tcccagccct ttccgtgagc ctccaggcag tgagcgggtg ctaacaacca
1321gcccctcacg ccaactggtg ggccgaacat tttcagatgg gttagccacc cgtaccctgc
1381agcctcctga gagtccccgc ctgggccggc ggggcctgga cagtatgcga gaactacccc
1441ccttaagtcc atctctgtcc cggcgagctc tctccccgct gcccacccgg accaccccag
1501atcccaagct aaacagggaa gtggcagaga gtcctcggcc ccggcgctgg gcagcccatg
1561gggcttcacc agaggacttc tccctgacgc tgggggcacg gggccgtagg acacggagcc
1621cctcacccac actgggtgag tctctggcac cccacaaggg cagcttcagt ggcaggctga
1681gcccagccta cagtctgggc tctcttactg gggcttcacc ctgccagagt ccctgtgtcc
1741agaggaagct ctccagcggg gacttgcggg tgcctgtcac aagggagcgg aaaaatagca
1801tcacagagat cagtgacaat gaggacgacc tcctggagta ccaccggcga cagcgccaag
1861agcggctccg ggagcaggag atggagaggc tggaacgcca gcgcctggag accatcctga
1921acctgtgtgc cgaatacagc cgggctgatg ggggacctga ggctggggag cttcccagca
1981ttggggaggc caccgcagca ttggcactgg caggccggag gccctcacga ggccttgcag
2041gggcctctgg gcggagcagc gaggagcctg gcgttgccac ccaacgccta tgggagagta
2101tggagcgctc agatgaggaa aatctcaagg aggagtgcag cagcactgag agcacccagc
2161aggagcacga agatgcacct agcaccaagc tccagggaga ggtgctagcc ctggaagaag
2221agcgggctca ggtgctgggg cacgtggagc agctcaaggt ccgtgtgaag gagctagagc
2281agcagctgca ggagtcagcc cgagaggccg aaatggagcg ggcactgctg cagggagaga
2341gggaggcaga gcgggcactg ctgcagaagg agcagaaggc agtggatcag ctgcaggaga
2401agctggtggc cttggagaca ggcatccaga aggagaggga caaggaggca gaggccctgg
2461agactgagac aaagctcttt gaggacttgg agttccagca gttggagcgg gagagccgcg
2521tggaggagga gcgcgagctg gccggccagg ggctgctccg gagcaaggct gagctgctcc
2581gcagcatcgc caagaggaag gagcgcctgg ccatcctgga cagtcaggct gggcagatcc
2641gggctcaggc cgtgcaggag tcagaacgcc tggcccggga caagaatgcc tccttacagc
2701tgctgcaaaa ggagaaggag aagctgactg tgctggaaag gagataccac tcactcacag
2761ggggcaggcc tttcccgaag accacatcga ccctcaaaga ggtttaccgc tccaagatgg
2821atggcgaggc caccagcccc cttccccgga cccgcagcgg ccccctcccc tcctcctctg
2881gctcttcctc ctcctcctcc cagctcagcg tggctaccct ggggcgtagc ccctccccaa
2941agagcgctct actcacccag aatggcacgg gcagccttcc tcgcaacctg gcagccacac
3001tgcaggacat cgagaccaag cgccaactag ctctgcagca gaagggacaa caagtgattg
3061aagagcagcg gcggcgactg gctgagctga agcagaaagc ggcagctgag gcacagtgcc
3121agtgggatgc ccttcacggg gcagcaccct tcccagcggg cccctcgggc ttcccccctc
3181tcatgcacca ctctatccta caccacctgc ctgcggggcg ggagcgtggg gaggagggtg
3241agcacgccta tgatacgctg agtctggaga gctctgacag catggagacc agcatctcca
3301ccgggggcaa ctcggcctgc tcccctgaca acatgtccag cgcgagtggt ctggacatgg
3361ggaagatcga ggagatggag aagatgctga aagaggctca tgcagagaag aaccggctca
3421tggagtcgag ggagcgggag atggagctgc ggcggcaggc cctggaggag gagcggcgga
3481ggcgtgagca ggtagaacgg aggctgcaga gtgagagtgc ccggaggcag cagctggtcg
3541agaaggaggt caagatgcgg gagaaacaat tttcccaggc acgacccctg acccgctacc
3601tgccaatccg gaaggaggac tttgacctga agacacatat tgagtcatcg ggccatggtg
3661ttgatacctg cctgcacgtg gtgcTCAGCA GCAAGGTCTG CCGTGGCTAC TTGGTCAAGA
3721TGGGCGGCAA GATTAAATCA TGGAAGAAGC GCTGGTTTGT CTTCGACCGG CTCAAGCGCA
3781CCCTTTCCTA TTATGTGGAC AAGCATGAGA CGAAGCTGAA GGGAGTCATC TATTTCCAGG
3841CCATTGAGGA AGTGTACTAC GACCACCTGC GCAGTGCAGC CAAGAGCCCG AACCCAGCCC
3901TCACCTTCTG CGTAAAGACC CATGACCGGC TGTACTACAT GGTGGCCCCA TCTGCAGAGG
3961CCATGCGTAT CTGGATGGAT GTCATTGTCA CAGGGGCTGA GGGCTACACT CAGTTCATGA
4021ACTAAACTAG TGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
4081ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
4141CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTG AACTGGGGGT AGCATCTTAT
4201CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)