Construct: ORF TRCN0000488391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021943.1_s317c1
- DNA Barcode:
- ACTAGCAATTCCGCAGTGTATGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2236_2250del | 99.9% | 100% | 2346G>A;3294C>A |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 99.8% | 99.9% | 2236_2237delGAinsAC;2346G>A;3294C>A |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 99.5% | 99.5% | (many diffs) |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 95.7% | 95.7% | (many diffs) |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 95.1% | 95.2% | (many diffs) |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 92.2% | 89.9% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 88.5% | 86.2% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 76.9% | 74.7% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.3% | 50.9% | (many diffs) |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 52% | 52% | 1881G>A;1883C>G;1884_1885ins1731 |
| 11 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 33.5% | 33.4% | 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2399 |
| 12 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 84.3% | 89.9% | (many diffs) |
| 13 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 45.1% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3687
- ORF length:
- 3615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg ctgctggctg 121 cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc acgagtaaca 181 agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg atgttcaata 241 actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat tatgatcttt 301 ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc aacacagtgg 361 agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac gaaaattcct 421 atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag gagctgccca 481 tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt 541 gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc agcaacatgt 601 cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg 661 ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc atctgtgccc 721 agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac aaccagtgtg 781 ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa ttccgagacg 841 aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc acgtaccaga 901 tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc 961 gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg 1021 agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt 1081 gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta 1141 aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta 1201 ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa 1261 ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc 1321 tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt 1381 ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa 1441 gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact 1501 ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa 1561 acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg 1621 gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg 1681 acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac 1741 agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag 1801 acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg 1861 caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt 1921 gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc 1981 caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc 2041 tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc 2101 gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag 2161 aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag 2221 tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga 2281 aagttaaaat tcccgtcgct atcaagacat ctccgaaagc caacaaggaa atcctcgatg 2341 aagcctacgt gatggccagc gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc 2401 tcacctccac cgtgcaactc atcacgcagc tcatgccctt cggctgcctc ctggactatg 2461 tccgggaaca caaagacaat attggctccc agtacctgct caactggtgt gtgcagatcg 2521 caaagggcat gaactacttg gaggaccgtc gcttggtgca ccgcgacctg gcagccagga 2581 acgtactggt gaaaacaccg cagcatgtca agatcacaga ttttgggctg gccaaactgc 2641 tgggtgcgga agagaaagaa taccatgcag aaggaggcaa agtgcctatc aagtggatgg 2701 cattggaatc aattttacac agaatctata cccaccagag tgatgtctgg agctacgggg 2761 tgaccgtttg ggagttgatg acctttggat ccaagccata tgacggaatc cctgccagcg 2821 agatctcctc catcctggag aaaggagaac gcctccctca gccacccata tgtaccatcg 2881 atgtctacat gatcatggtc aagtgctgga tgatagacgc agatagtcgc ccaaagttcc 2941 gtgagttgat catcgaattc tccaaaatgg cccgagaccc ccagcgctac cttgtcattc 3001 agggggatga aagaatgcat ttgccaagtc ctacagactc caacttctac cgtgccctga 3061 tggatgaaga agacatggac gacgtggtgg atgccgacga gtacctcatc ccacagcagg 3121 gcttcttcag cagcccctcc acgtcacgga ctcccctcct gagctctctg agtgcaacca 3181 gcaacaattc caccgtggct tgcattgata gaaatgggct gcaaagctgt cccatcaagg 3241 aagacagctt cttgcagcga tacagctcag accccacagg cgccttgact gaggacagca 3301 tagacgacac cttcctccca gtgcctgaat acataaacca gtccgttccc aaaaggcccg 3361 ctGGATCTGT GCAGAATCCT GTCTATCACA ATCAGCCTCT GAACCCCGCG CCCAGCAGAG 3421 ACCCACACTA CCAGGACCCC CACAGCACTG CAGTGGGCAA CCCCGAGTAT CTCAACACTG 3481 TCCAGCCCAC CTGTGTCAAC AGCACATTCG ACAGCCCTGC CCACTGGGCC CAGAAAGGCA 3541 GCCACCAAAT TAGCCTGGAC AACCCTGACT ACCAGCAGGA CTTCTTTCCC AAGGAAGCCA 3601 AGCCAAATGG CATCTTTAAG GGCTCCACAG CTGAAAATGC AGAATACCTA AGGGTCGCGC 3661 CACAAAGCAG TGAATTTATT GGAGCATGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 3721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACTAG 3841 CAATTCCGCA GTGTATGAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3901 tgaaagatt