Construct: ORF TRCN0000488487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020231.1_s317c1
- DNA Barcode:
- CCACACGCATTACAACTTATGGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADK (132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488487
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 132 | ADK | adenosine kinase | NM_001123.3 | 99.9% | 99.7% | 604A>T |
| 2 | human | 132 | ADK | adenosine kinase | NM_006721.4 | 94.8% | 93.9% | (many diffs) |
| 3 | human | 132 | ADK | adenosine kinase | NM_001202449.1 | 94.6% | 94.4% | 89_90ins54;550A>T |
| 4 | human | 132 | ADK | adenosine kinase | NM_001369124.1 | 83.4% | 83.4% | 502_503ins171 |
| 5 | human | 132 | ADK | adenosine kinase | XM_017015699.1 | 83.3% | 76.5% | (many diffs) |
| 6 | human | 132 | ADK | adenosine kinase | XM_017015702.1 | 80.1% | 68.7% | 604A>T;709_710ins115;831_832ins89 |
| 7 | human | 132 | ADK | adenosine kinase | XM_017015698.1 | 79.2% | 75.5% | (many diffs) |
| 8 | human | 132 | ADK | adenosine kinase | NM_001202450.1 | 79.1% | 78.4% | (many diffs) |
| 9 | human | 132 | ADK | adenosine kinase | XM_017015703.2 | 78.1% | 77.9% | 0_1ins225;379A>T |
| 10 | human | 132 | ADK | adenosine kinase | NM_001369123.1 | 76% | 64.4% | (many diffs) |
| 11 | human | 132 | ADK | adenosine kinase | XM_017015706.1 | 65.7% | 64.9% | (many diffs) |
| 12 | human | 132 | ADK | adenosine kinase | XM_017015704.1 | 62.3% | 60.7% | (many diffs) |
| 13 | human | 132 | ADK | adenosine kinase | XM_017015705.1 | 60.4% | 49.3% | (many diffs) |
| 14 | mouse | 11534 | Adk | adenosine kinase | NM_001243041.1 | 89.2% | 91% | (many diffs) |
| 15 | mouse | 11534 | Adk | adenosine kinase | NM_134079.4 | 85% | 86.1% | (many diffs) |
| 16 | mouse | 11534 | Adk | adenosine kinase | XM_006518440.3 | 59% | 56.1% | (many diffs) |
| 17 | mouse | 11534 | Adk | adenosine kinase | XM_006518441.3 | 55.4% | 55.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1107
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacgtca gtcagagaaa atattctctt tggaatggga aatcctctgc 121 ttgacatctc tgctgtagtg gacaaagatt tccttgataa gtattctctg aaaccaaatg 181 accaaatctt ggctgaagac aaacacaagg aactgtttga tgaacttgtg aaaaaattca 241 aagtcgaata tcatgctggt ggctctaccc agaattcaat taaagtggct cagtggatga 301 ttcaacagcc acacaaagca gcaacatttt ttggatgcat tgggatagat aaatttgggg 361 agatcctgaa gagaaaagct gctgaagccc atgtggatgc tcattactac gagcagaatg 421 agcagccaac aggaacttgt gctgcatgca tcactggtga caacaggtcc ctcatagcta 481 atcttgctgc tgccaattgt tataaaaagg aaaaacatct tgatctggag aaaaactgga 541 tgttggtaga aaaagcaaga gtttgttata tagcaggctt ttttcttaca gtttccccag 601 agtcagtatt aaaggtggct caccatgctt ctgaaaacaa caggattttc actttgaatc 661 tatctgcacc gttttttagc cagttctaca aggaatcatt gatgaaagtt atgccttatg 721 ttgatatact ttttggaaat gagacagaag ctgccacttt tgctagagag caaggctttg 781 agactaaaga CATTAAAGAG ATAGCCAAAA AGACACAAGC CCTGCCAAAG ATGAACTCAA 841 AGAGGCAGCG AATCGTGATC TTCACCCAAG GGAGAGATGA CACTATAATG GCTACAGAAA 901 GTGAAGTCAC TGCTTTTGCT GTCTTGGATC AAGACCAGAA AGAAATTATT GATACCAATG 961 GAGCTGGAGA TGCATTTGTT GGAGGTTTTC TGTCTCAACT GGTCTCTGAC AAGCCTCTGA 1021 CTGAATGTAT CCGTGCTGGC CACTATGCAG CAAGCATCAT AATTAGACGG ACTGGCTGCA 1081 CCTTTCCTGA GAAGCCAGAC TTCCACTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCACA 1261 CGCATTACAA CTTATGGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt