Construct: ORF TRCN0000488521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021478.1_s317c1
- DNA Barcode:
- GAACTTAAACATACCTTACTTGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PI4KA (5297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_058004.4 | 97.1% | 97.2% | (many diffs) |
| 2 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362863.1 | 96.1% | 96.1% | (many diffs) |
| 3 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362862.1 | 95.7% | 95.7% | (many diffs) |
| 4 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XR_937868.1 | 88.9% | (many diffs) | |
| 5 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_005261635.1 | 82.9% | 82.9% | (many diffs) |
| 6 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_017028830.1 | 71.8% | 71.8% | (many diffs) |
| 7 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_011530226.1 | 62.4% | 62.3% | (many diffs) |
| 8 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_011530228.2 | 55.8% | 55.8% | (many diffs) |
| 9 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XR_001755251.1 | 55.6% | (many diffs) | |
| 10 | human | 375133 | PI4KAP2 | phosphatidylinositol 4-kina... | NR_003700.1 | 28.1% | (many diffs) | |
| 11 | human | 728233 | PI4KAP1 | phosphatidylinositol 4-kina... | NR_003563.1 | 25.2% | (many diffs) | |
| 12 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | NM_001001983.2 | 89.2% | 97.6% | (many diffs) |
| 13 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522034.1 | 86.6% | 94.8% | (many diffs) |
| 14 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522036.3 | 56.3% | 61.2% | (many diffs) |
| 15 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522037.2 | 51.4% | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 78
- ORF end:
- 6210
- ORF length:
- 6132
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcga attcaccatg tgtccagtgg atttccatgg gatcttccag ttagatgaaa 121 gacggagaga tgcagtgatt gcattgggca tttttctgat tgaatctgat cttcagcaca 181 aagattgtgt ggttccttac cttcttcgac ttctcaaagg tcttccaaaa gtgtattggg 241 tagaagaaag cacagctcgg aaaggcagag gtgccctccc ggttgcagag agcttcagct 301 tctgcttggt aactctgctg tctgatgtgg cctataggga tccttcactt agggatgaga 361 ttttagaggt gcttttgcag gttttgcatg tcctcttggg gatgtgccag gccttggaga 421 ttcaagacaa agaatacctt tgcaagtatg ctatcccatg cctgatagga atctcgcgag 481 catttgggcg ttacagcaac atggaagagt ctctcctctc aaagctcttt cccaaaatcc 541 ctcctcattc cctccgtgtc ctggaagagc ttgaaggtgt tcgaaggcgt tcctttaatg 601 acttccgctc catcctcccc agcaatctgc tgactgtctg tcaggagggt accctgaaga 661 ggaaaaccag cagtgtgtcc agcatctctc aggtcagccc tgaacgcggc atgccccctc 721 ccagttcccc tggaggatct gcctttcact actttgaagc ctcctgtttg cccgatggga 781 ctgccctaga gcctgagtac tacttttcaa ccatcagctc cagcttctca gtctctcccc 841 ttttcaacgg tgtcacatat aaggagttta acattccatt ggaaatgctt cgggaactct 901 taaacctggt gaagaagatc gttgaggagg ctgttctcaa atctttggat gccattgtag 961 ccagtgtgat ggaggccaac cccagtgctg atctctacta cacttccttc agtgaccctc 1021 tctacctgac catgttcaag atgctgcgtg acactctgta ctacatgaag gacctcccga 1081 cctcttttgt gaaggagatc catgattttg tgctggagca gttcaacacg agccaggggg 1141 agctccagaa gattctacat gacgcagacc ggatccacaa tgagctgagc cccctcaaac 1201 tgcgctgtca ggcgaatgct gcctgtgtgg acctcatggt gtgggctgtg aaggacgagc 1261 agggtgcaga aaacctttgc atcaagctat ctgagaagct gcagtccaag acgtccagca 1321 aagtcattat tgctcacttg cccctgctga tctgctgtct gcagggtttg ggccgcctgt 1381 gcgagaggtt cccggtggtg gtgcactctg tgacaccgtc cttgcgagac ttcctggtca 1441 tcccgtcccc agttctggtg aagctctaca agtaccacag tcagtaccac acagttgctg 1501 gcaatgatat aaaaatcagt gtgaccaatg agcattccga gtcaaccctg aacgtcatgt 1561 cgggtaagaa gagccagccc tccatgtacg agcagctccg agacatcgct attgacaaca 1621 tctgcaggtg cctgaaggct ggattgacgg tggacccagt gattgtggag gcgttcttgg 1681 ccagcctgtc caaccggctc tacatctctc aggagagcga caaggacgct cacttgattc 1741 ccgaccacac aatccgagcc ttgggacaca ttgcggtggc cttgagggac accccgaagg 1801 tcatggagcc cattctgcag atcctacagc agaaattctg ccagccaccc tcccccctcg 1861 atgtgctgat tattgaccag ctgggctgcc tggttatcac cggaaatcaa tacatctatc 1921 aggaagtgtg gaacctcttc cagcagatca gtgtgaaggc cagctccgtt gtatactcag 1981 ccaccaaaga ttacaaggac cacggctata ggcattgctc cctggcagtg attaatgccc 2041 tggccaacat cgcggccaac atccaagacg agcacctggt ggatgagctg ctcatgaacc 2101 tgttggagtt gtttgtgcag ctggggctgg aggggaagcg agccagcgag agggcaagcg 2161 agaagggccc tgccctaaag gcttctagca gtgcagggaa cttgggagta ctcattcctg 2221 taatagctgt gctcacccga cgactgccac ccatcaaaga agctaagcct cggttacaga 2281 agctcttccg agacttctgg ctgtattccg ttctgatggg attcgctgtg gagggctcag 2341 gactctggcc agaagaatgg tacgaggggg tctgtgaaat agccactaag tcccccttgc 2401 tcacctttcc cagcaaggag ccactgcggt ccgtcctcca gtataactca gccatgaaga 2461 atgacacggt cacccccgct gagctgagtg agctccgcag cactatcatc aacctgctgg 2521 acccccctcc cgaggtgtcc gcactcatca acaagctgga cttcgccatg tccacctacc 2581 tcctctctgt gtaccggctg gagtacatga gggtactgcg ttcaacagat cctgatcgct 2641 tccaggtaat gttctgctac tttgaggata aagctattca gaaagacaaa tctgggatga 2701 tgcagtgtgt gattgcagtc gcggacaaag tattcgatgc cttcctgaac atgatggcgg 2761 ataaagccaa gaccaaggag aacgaggagg agctggagcg gcacgctcag ttcctgttgg 2821 tgaacttcaa ccacatccac aagaggataa ggagggtggc agacaagtat ctatctggtc 2881 tggtggataa gtttccccac ttgctctgga gcgggactgt gctgaagacc atgctggaca 2941 tcctgcagac cctgtcactg tcactgagcg ctgatattca caaggatcag ccttactatg 3001 acatccccga cgccccctac cggatcacgg ttcctgacac gtacgaagcc cgtgagagca 3061 ttgtgaagga cttcgctgca cgctgtggga tgatcctcca ggaggccatg aagtgggcac 3121 ctaccgtcac caagtcccac ctgcaggaat atctgaacaa acatcagaac tgggtatcgg 3181 gactgtccca gcacacgggg ctggccatgg ccactgagag catccttcac tttgctggct 3241 acaacaagca gaacacaact cttggggcaa ctcagctgag cgagcgcccg gcctgtgtga 3301 agaaagacta ctccaacttc atggcatccc tgaatctgcg caaccgctac gcgggcgagg 3361 tgtatggaat gattcggttc tcaggcacca caggccagat gtctgacctg aacaaaatga 3421 tggtccagga tctacattca gctttagacc gcagtcatcc tcagcactac acgcaggcca 3481 tgttcaagct gaccgcaatg ctcattagca gtaaagattg tgacccgcag ctccttcatc 3541 atctgtgctg gggtcccctc cggatgttca atgagcatgg catggagacg gccctggcct 3601 gctgggagtg gctgctggct ggcaaggatg gagtggaagt gccgttcatg cgggagatgg 3661 caggggcctg gcacatgacg gtggagcaga aatttggcct gttttctgct gagataaagg 3721 aagcagaccc cctggctgcc tcggaagcaa gtcaacccaa accctgtccc cccgaagtga 3781 ccccccacta catctggatc gacttcctgg tgcagcggtt tgagatcgcc aagtactgca 3841 gctctgacca agtggagatc ttctccagcc tgctgcagcg ctccatgtcc ctgaacatcg 3901 gcggggccaa ggggagcatg aaccggcacg tggcggccat cgggccccgc ttcaagctgc 3961 tgaccctggg gctgtccctc ctgcatgccg atgtggttcc aaatgcaacc atccgcaatg 4021 tgcttcgcga gaagatctac tccactgcct ttgactactt cagctgtccc ccaaagttcc 4081 ctactcaagg agagaagcgg ctgcgtgaag acataagcat catgattaaa ttttggaccg 4141 ccatgttctc agataagaag tacctgaccg ccagccagct tgttccccca gataatcagg 4201 acacccggag caacctggac ataactgtcg gctctcggca acaagccacc caaggctgga 4261 tcaacacata ccccctgtcc agcggcatgt ccaccatctc caagaaatca ggcatgtcta 4321 agaaaaccaa ccggggctcc cagctgcaca aatactacat gaagcgcagg acgctgctgc 4381 tgtccctgct ggccactgag atcgagcgtc tcatcacatg gtacaacccg ctgtcagccc 4441 cggaactgga actagaccag gccggagaga acagcgtggc caactggaga tctaagtaca 4501 tcagcctgag tgagaagcag tggaaggaca acgtgaacct cgcctggagc atctctccct 4561 acctagccgt gcagctgcct gccaggttta agaacacaga agccattggg aacgaagtga 4621 cccgtctcgt tcggttggac ccgggagccg ttagtgatgt gcctgaagca atcaagttcc 4681 tggtcacctg gcacaccatc gacgccgatg ctccagagct cagccatgtg ctgtgctggg 4741 cgcccacgga cccacccaca ggcctctcct acttctccag catgtacccg ccgcaccctc 4801 tcacggcgca gtacggggtg aaagtcctgc ggtccttccc tccggacgcc atcctcttct 4861 acatccccca gattgtgcag gccctcaggt acgacaagat gggctatgtg cgggagtata 4921 ttctgtgggc agcgtctaaa tcccagcttc tggcacacca gttcatctgg aacatgaaga 4981 ctaacattta tctagatgaa gagggccacc agaaagaccc tgacatcggc gacctcctgg 5041 atcagttggt agaggagatc acaggctcct tgtccggccc agcgaaggac ttttaccagc 5101 gggagtttga tttctttaac aagatcacca acgtgtcggc tatcatcaag ccctacccta 5161 aaggcgacga gagaaagaag gcttgtctgt cggccctgtc tgaagtgaag gtgcagccgg 5221 gctgctacct gcccagcaac cctgaggcca ttgtgctgga catcgactac aagtctggga 5281 ccccgatgca gagtgctgca aaagccccat atctggccaa gttcaaggtg aagcgatgtg 5341 gagttagtga acttgaaaaa gaaggtctgc ggtgccgctc agactccgag gatgagtgca 5401 gcacgcagga ggccgacggc cagaagatct cctggcaggc agccatcttc aaggtgggag 5461 acgactgccg gcaggacatg ctggccctgc agatcatcga cctcttcaag aacatcttcc 5521 agctggtcgg cctggacctc tttgtttttc cctaccgcgt ggtggccact gcccctgggt 5581 gtggggtgat cgagtgcatc cccgactgca cctcccggga ccagctgggc cgccagacag 5641 acttcggcat gtacgactac ttcacacgcc agtacgggga tgagtccacc ctggccttcc 5701 agcaggcccg ctacaacttc atccgaagca tggccgccta cagcctcctg ctgttcctgc 5761 tgcagatcaa ggacagacac aacggcaaca ttatgctgga caagaagggc catatcatcc 5821 acatcgactt tGGCTTCATG TTTGAAAGCT CGCCGGGCGG CAATCTCGGC TGGGAACCCG 5881 ACATCAAGCT GACGGATGAG ATGGTGATGA TCATGGGGGG CAAGATGGAG GCCACACCCT 5941 TCAAGTGGTT CATGGAGATG TGTGTCCGAG GCTACCTGGC TGTGCGGCCC TACATGGACG 6001 CGGTCGTCTC CCTGGTCACT CTCATGTTGG ACACGGGCCT GCCCTGTTTT CGCGGCCAGA 6061 CAATCAAGCT CTTGAAGCAC AGGTTTAGCC CCAACATGAC TGAGCGCGAG GCTGCAAATT 6121 TCATCATGAA GGTCATCCAG AGCTGCTTCC TCAGCAACAG GAGCCGGACC TACGACATGA 6181 TCCAGTACTA TCAGAATGAC ATCCCCTACT GAAAGCTTGA CCCAGCTTTC TTGTACAAAG 6241 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 6301 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 6361 ACGAGAACTT AAACATACCT TACTTGCCAC GCGTTAAGTC gacaatcaac ctctggatta 6421 caaaatttgt gaaagatt