Construct: ORF TRCN0000488623
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019560.1_s317c1
- DNA Barcode:
- CGGACCAGCGCATGACCTTCAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NTRK1 (4914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488623
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001012331.1 | 96.7% | 96.6% | 0_1ins78;787G>T;1656G>A |
| 2 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_002529.3 | 96% | 95.9% | (many diffs) |
| 3 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001007792.1 | 90.9% | 86.4% | (many diffs) |
| 4 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | XM_006501124.2 | 82% | 83.5% | (many diffs) |
| 5 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | NM_001033124.1 | 81.4% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 2538
- ORF length:
- 2448
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgaa ttcgcgcaca tgtcgggtga ggcctggcag ctgcagctgg 121 gagcgcacag acggctgccc cgcctgagcg aggcgggcgc cgccgcgatg ctgcgaggcg 181 gacggcgcgg gcagcttggc tggcacagct gggctgcggg gccgggcagc ctgctggctt 241 ggctgatact ggcatctgcg ggcgccgcac cctgccccga tgcctgctgc ccccacggct 301 cctcgggact gcgatgcacc cgggatgggg ccctggatag cctccaccac ctgcccggcg 361 cagagaacct gactgagctc tacatcgaga accagcagca tctgcagcat ctggagctcc 421 gtgatctgag gggcctgggg gagctgagaa acctcaccat cgtgaagagt ggtctccgtt 481 tcgtggcgcc agatgccttc catttcactc ctcggctcag tcgcctgaat ctctccttca 541 acgctctgga gtctctctcc tggaaaactg tgcagggcct ctccttacag gaactggtcc 601 tgtcggggaa ccctctgcac tgttcttgtg ccctgcgctg gctacagcgc tgggaggagg 661 agggactggg cggagtgcct gaacagaagc tgcagtgtca tgggcaaggg cccctggccc 721 acatgcccaa tgccagctgt ggtgtgccca cgctgaaggt ccaggtgccc aatgcctcgg 781 tggatgtggg ggacgacgtg ctgctgcggt gccaggtgga ggggcggggc ctggagcagg 841 ccggctggat cctcacagag ctggagcagt cagccacggt gatgaaatct gggggtctgc 901 catccctggg gctgaccctg gccaatgtca ccagtgacct caacaggaag aacttgacgt 961 gctgggcaga gaacgatgtg ggccgggcag aggtctctgt tcaggtcaac gtctccttcc 1021 cggccagtgt gcagctgcac acggcggtgg agatgcacca ctggtgcatc cccttctctg 1081 tggatgggca gccggcaccg tctctgcgct ggctcttcaa tggctccgtg ctcaatgaga 1141 ccagcttcat cttcactgag ttcctggagc cggcagccaa tgagaccgtg cggcacgggt 1201 gtctgcgcct caaccagccc acccacgtca acaacggcaa ctacacgctg ctggctgcca 1261 accccttcgg ccaggcctcc gcctccatca tggctgcctt catggacaac cctttcgagt 1321 tcaaccccga ggaccccatc cctgacacta acagcacatc tggagacccg gtggagaaga 1381 aggacgaaac accttttggg gtctcggtgg ctgtgggcct ggccgtcttt gcctgcctct 1441 tcctttctac gctgctcctt gtgctcaaca aatgtggacg gagaaacaag tttgggatca 1501 accgcccggc tgtgctggct ccagaggatg ggctggccat gtccctgcat ttcatgacat 1561 tgggtggcag ctccctgtcc cccaccgagg gcaaaggctc tgggctccaa ggccacatca 1621 tcgagaaccc acaatacttc agtgatgcct gtgttcacca catcaagcgc cgggacatcg 1681 tgctcaagtg ggagctgggg gagggcgcct ttgggaaggt cttccttgct gagtgccaca 1741 acctcctgcc tgagcaggac aagatgctgg tggctgtcaa ggcactgaag gaggcgtccg 1801 agagtgctcg gcaggacttc caacgtgagg ctgagctgct caccatgctg cagcaccagc 1861 acatcgtgcg cttcttcggc gtctgcaccg agggccgccc cctgctcatg gtctttgagt 1921 atatgcggca cggggacctc aaccgcttcc tccgatccca tggacctgat gccaagctgc 1981 tggctggtgg ggaggatgtg gctccaggcc ccctgggtct ggggcagctg ctggccgtgg 2041 ctagccaggt cgctgcgggg atggtgtacc tggcgggtct gcattttgtg caccgggacc 2101 tggccacacg caactgtcta gtgggccagg gactggtggt caagattggt gattttggca 2161 tgagcaggga tatctacagc accgactatt accgtgtggg aggccgcacc atgctgccca 2221 ttcgctggat gccgcccgag agcatcctgt accgtaagtt caccaccgag agcgacgtgt 2281 ggagcTTCGG CGTGGTGCTC TGGGAGATCT TCACCTACGG CAAGCAGCCC TGGTACCAGC 2341 TCTCCAACAC GGAGGCAATC GACTGCATCA CGCAGGGACG TGAGTTGGAG CGGCCACGTG 2401 CCTGCCCACC AGAGGTCTAC GCCATCATGC GGGGCTGCTG GCAGCGGGAG CCCCAGCAAC 2461 GCCACAGCAT CAAGGATGTG CACGCCCGGC TGCAAGCCCT GGCCCAGGCA CCTCCTGTCT 2521 ACCTGGATGT CCTGGGCAAG GGTGGGCGCG CCGACCCAGC TTTCTTGTAC AAAGTGGTTG 2581 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2641 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 2701 GACCAGCGCA TGACCTTCAA GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2761 ttgtgaaaga tt