Construct: ORF TRCN0000488713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020612.1_s317c1
- DNA Barcode:
- AAGAGGCTTCCCATAGTAATAAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FGFR1 (2260)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174063.2 | 100% | 100% | |
| 2 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023110.3 | 99.7% | 99.7% | 1279_1284delGTAACA |
| 3 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013224.2 | 99.7% | 99.7% | 441_442insCGTATG |
| 4 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013225.2 | 99.7% | 99.7% | 441_442insCGTATG |
| 5 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174065.2 | 99.5% | 99.5% | 441_442insCGTATG;1273_1278delGTAACA |
| 6 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_015850.4 | 99.5% | 99.5% | 441_442insCGTATG;1273_1278delGTAACA |
| 7 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174064.2 | 98.2% | 96.1% | (many diffs) |
| 8 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544447.2 | 96.1% | 96.1% | 1_99del |
| 9 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544446.2 | 95.9% | 95.9% | 1_99del;1378_1383delGTAACA |
| 10 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013220.1 | 95.8% | 95.8% | 1_99del;540_541insCGTATG |
| 11 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174067.1 | 95.6% | 95.6% | 1_99del;540_541insCGTATG;1372_1377delGTAACA |
| 12 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013222.2 | 94.6% | 91% | (many diffs) |
| 13 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716303.3 | 94.4% | 90.8% | (many diffs) |
| 14 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716304.1 | 94.4% | 90.8% | (many diffs) |
| 15 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013221.1 | 94.4% | 90.8% | (many diffs) |
| 16 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354369.1 | 94.4% | 90.8% | (many diffs) |
| 17 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354367.1 | 94.1% | 90.6% | (many diffs) |
| 18 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716307.1 | 94.1% | 90.6% | (many diffs) |
| 19 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_024447097.1 | 92.6% | 87.2% | (many diffs) |
| 20 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544445.2 | 91% | 87.6% | (many diffs) |
| 21 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544443.2 | 90.8% | 87.4% | (many diffs) |
| 22 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013219.1 | 90.8% | 87.4% | (many diffs) |
| 23 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544444.1 | 90.6% | 87.2% | (many diffs) |
| 24 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174066.2 | 88.9% | 88.9% | 89_90ins267;1012_1017delGTAACA |
| 25 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023105.3 | 88.9% | 88.9% | 89_90ins267;1012_1017delGTAACA |
| 26 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354368.2 | 88.9% | 88.9% | 89_90ins267;174_175insCGTATG |
| 27 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023106.3 | 88.6% | 88.6% | 89_90ins267;174_175insCGTATG;1006_1011delGTAACA |
| 28 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013226.1 | 85.6% | 85.6% | 1_99del;188_189ins267 |
| 29 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013227.1 | 85.4% | 85.4% | 1_99del;188_189ins267;273_274insCGTATG |
| 30 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544450.2 | 85.2% | 85.2% | (many diffs) |
| 31 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716310.3 | 83.8% | 80.4% | (many diffs) |
| 32 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716311.1 | 83.8% | 80.4% | (many diffs) |
| 33 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716312.2 | 83.8% | 80.4% | (many diffs) |
| 34 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354370.1 | 83.6% | 80.1% | (many diffs) |
| 35 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716314.2 | 83.6% | 80.1% | (many diffs) |
| 36 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544451.1 | 82.2% | 76.9% | (many diffs) |
| 37 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544448.1 | 80.7% | 77.4% | (many diffs) |
| 38 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544449.1 | 80.4% | 77.2% | (many diffs) |
| 39 | human | 2260 | FGFR1 | fibroblast growth factor re... | XR_001745495.1 | 67.5% | (many diffs) | |
| 40 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013230.1 | 60.7% | 60.7% | 0_1ins966 |
| 41 | human | 2260 | FGFR1 | fibroblast growth factor re... | XR_001745496.1 | 58.9% | (many diffs) | |
| 42 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013229.2 | 56.3% | 53.2% | (many diffs) |
| 43 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013231.1 | 47.3% | 37.8% | (many diffs) |
| 44 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544452.2 | 47.1% | 37.5% | (many diffs) |
| 45 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_011242120.1 | 89.8% | 98.4% | (many diffs) |
| 46 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_010206.3 | 89.6% | 98.1% | (many diffs) |
| 47 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509010.2 | 89.6% | 98.1% | (many diffs) |
| 48 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509012.1 | 89.6% | 98.1% | (many diffs) |
| 49 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_001079908.2 | 89.4% | 98% | (many diffs) |
| 50 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509013.1 | 89.4% | 98% | (many diffs) |
| 51 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_001079909.2 | 79.8% | 87.9% | (many diffs) |
| 52 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_011242121.1 | 79.6% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2532
- ORF length:
- 2460
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtggagc tggaagtgcc tcctcttctg ggctgtgctg gtcacagcca 121 cactctgcac cgctaggccg tccccgacct tgcctgaaca agcccagccc tggggagccc 181 ctgtggaagt ggagtccttc ctggtccacc ccggtgacct gctgcagctt cgctgtcggc 241 tgcgggacga tgtgcagagc atcaactggc tgcgggacgg ggtgcagctg gcggaaagca 301 accgcacccg catcacaggg gaggaggtgg aggtgcagga ctccgtgccc gcagactccg 361 gcctctatgc ttgcgtaacc agcagcccct cgggcagtga caccacctac ttctccgtca 421 atgtttcaga tgctctcccc tcctcggagg atgatgatga tgatgatgac tcctcttcag 481 aggagaaaga aacagataac accaaaccaa accgtatgcc cgtagctcca tattggacat 541 ccccagaaaa gatggaaaag aaattgcatg cagtgccggc tgccaagaca gtgaagttca 601 aatgcccttc cagtgggacc ccaaacccca cactgcgctg gttgaaaaat ggcaaagaat 661 tcaaacctga ccacagaatt ggaggctaca aggtccgtta tgccacctgg agcatcataa 721 tggactctgt ggtgccctct gacaagggca actacacctg cattgtggag aatgagtacg 781 gcagcatcaa ccacacatac cagctggatg tcgtggagcg gtcccctcac cggcccatcc 841 tgcaagcagg gttgcccgcc aacaaaacag tggccctggg tagcaacgtg gagttcatgt 901 gtaaggtgta cagtgacccg cagccgcaca tccagtggct aaagcacatc gaggtgaatg 961 ggagcaagat tggcccagac aacctgcctt atgtccagat cttgaagact gctggagtta 1021 ataccaccga caaagagatg gaggtgcttc acttaagaaa tgtctccttt gaggacgcag 1081 gggagtatac gtgcttggcg ggtaactcta tcggactctc ccatcactct gcatggttga 1141 ccgttctgga agccctggaa gagaggccgg cagtgatgac ctcgcccctg tacctggaga 1201 tcatcatcta ttgcacaggg gccttcctca tctcctgcat ggtggggtcg gtcatcgtct 1261 acaagatgaa gagtggtacc aagaagagtg acttccacag ccagatggct gtgcacaagc 1321 tggccaagag catccctctg cgcagacagg tgtctgctga ctccagtgca tccatgaact 1381 ctggggttct tctggttcgg ccatcacggc tctcctccag tgggactccc atgctagcag 1441 gggtctctga gtatgagctt cccgaagacc ctcgctggga gctgcctcgg gacagactgg 1501 tcttaggcaa acccctggga gagggctgct ttgggcaggt ggtgttggca gaggctatcg 1561 ggctggacaa ggacaaaccc aaccgtgtga ccaaagtggc tgtgaagatg ttgaagtcgg 1621 acgcaacaga gaaagacttg tcagacctga tctcagaaat ggagatgatg aagatgatcg 1681 ggaagcataa gaatatcatc aacctgctgg gggcctgcac gcaggatggt cccttgtatg 1741 tcatcgtgga gtatgcctcc aagggcaacc tgcgggagta cctgcaggcc cggaggcccc 1801 cagggctgga atactgctac aaccccagcc acaacccaga ggagcagctc tcctccaagg 1861 acctggtgtc ctgcgcctac caggtggccc gaggcatgga gtatctggcc tccaagaagt 1921 gcatacaccg agacctggca gccaggaatg tcctggtgac agaggacaat gtgatgaaga 1981 tagcagactt tggcctcgca cgggacattc accacatcga ctactataaa aagacaacca 2041 acggccgact gcctgtgaag tggatggcac ccgaggcatt atttgaccgg atctacaccc 2101 accagagtga tgtgtggtct ttcggggtgc tcctgtggga gatcttcact ctgggcggct 2161 ccccataccc cggtgtgcct gtggaggaac ttttCAAGCT GCTGAAGGAG GGTCACCGCA 2221 TGGACAAGCC CAGTAACTGC ACCAACGAGC TGTACATGAT GATGCGGGAC TGCTGGCATG 2281 CAGTGCCCTC ACAGAGACCC ACCTTCAAGC AGCTGGTGGA AGACCTGGAC CGCATCGTGG 2341 CCTTGACCTC CAACCAGGAG TACCTGGACC TGTCCATGCC CCTGGACCAG TACTCCCCCA 2401 GCTTTCCCGA CACCCGGAGC TCTACGTGCT CCTCAGGGGA GGATTCCGTC TTCTCTCATG 2461 AGCCGCTGCC CGAGGAGCCC TGCCTGCCCC GACACCCAGC CCAGCTTGCC AATGGCGGAC 2521 TCAAACGCCG CTAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2581 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2641 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAGAGGCTTC CCATAGTAAT 2701 AAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt