Construct: ORF TRCN0000488857
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021469.1_s317c1
- DNA Barcode:
- GGCTGTCCTGGAAACAGACATATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPARG (5468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488857
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354666.2 | 99.2% | 99.5% | 1_6delATGACC;1431_1432insTAGGC |
| 2 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354667.2 | 99.2% | 99.5% | 1_6delATGACC;1431_1432insTAGGC |
| 3 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374263.1 | 99.2% | 99.5% | 1_6delATGACC;1431_1432insTAGGC |
| 4 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374264.1 | 99.2% | 99.5% | 1_6delATGACC;1431_1432insTAGGC |
| 5 | human | 5468 | PPARG | peroxisome proliferator act... | NM_005037.6 | 99.2% | 99.5% | 1_6delATGACC;1431_1432insTAGGC |
| 6 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138711.4 | 99.2% | 99.5% | 1_6delATGACC;1431_1432insTAGGC |
| 7 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138712.4 | 99.2% | 99.5% | 1_6delATGACC;1431_1432insTAGGC |
| 8 | human | 5468 | PPARG | peroxisome proliferator act... | NM_015869.5 | 93.7% | 94% | 1_90del;1515_1516insTAGGC |
| 9 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354669.2 | 55.8% | 47.8% | 0_1ins427;102_103ins200;798_799insTAGGC |
| 10 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354670.2 | 54% | 51.6% | (many diffs) |
| 11 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354668.2 | 51.1% | 48.8% | (many diffs) |
| 12 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001330615.2 | 51% | 50.9% | (many diffs) |
| 13 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374261.1 | 51% | 50.9% | (many diffs) |
| 14 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374262.1 | 51% | 50.9% | (many diffs) |
| 15 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374265.1 | 48.2% | 48.1% | (many diffs) |
| 16 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374266.1 | 45.8% | 45.9% | (many diffs) |
| 17 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001127330.2 | 90.3% | 98.3% | (many diffs) |
| 18 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308352.1 | 90.3% | 98.3% | (many diffs) |
| 19 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308354.1 | 90.3% | 98.3% | (many diffs) |
| 20 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505743.3 | 90.3% | 98.3% | (many diffs) |
| 21 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_011241252.1 | 90.3% | 98.3% | (many diffs) |
| 22 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321455.1 | 90.3% | 98.3% | (many diffs) |
| 23 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505737.3 | 85.8% | 93.4% | (many diffs) |
| 24 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505738.3 | 85.8% | 93.4% | (many diffs) |
| 25 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505739.2 | 85.8% | 93.4% | (many diffs) |
| 26 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_011146.3 | 85% | 92.4% | (many diffs) |
| 27 | mouse | 19016 | Pparg | peroxisome proliferator act... | XR_001785108.1 | 61.6% | (many diffs) | |
| 28 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321456.1 | 55.8% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 64
- ORF end:
- 1489
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcat 61 accatggttg acacagagat gccattctgg cccaccaact ttgggatcag ctccgtggat 121 ctctccgtaa tggaagacca ctcccactcc tttgatatca agcccttcac tactgttgac 181 ttctccagca tttctactcc acattacgaa gacattccat tcacaagaac agatccagtg 241 gttgcagatt acaagtatga cctgaaactt caagagtacc aaagtgcaat caaagtggag 301 cctgcatctc caccttatta ttctgagaag actcagctct acaataagcc tcatgaagag 361 ccttccaact ccctcatggc aattgaatgt cgtgtctgtg gagataaagc ttctggattt 421 cactatggag ttcatgcttg tgaaggatgc aagggtttct tccggagaac aatcagattg 481 aagcttatct atgacagatg tgatcttaac tgtcggatcc acaaaaaaag tagaaataaa 541 tgtcagtact gtcggtttca gaaatgcctt gcagtgggga tgtctcataa tgccatcagg 601 tttgggcgga tgccacaggc cgagaaggag aagctgttgg cggagatctc cagtgatatc 661 gaccagctga atccagagtc cgctgacctc cgggccctgg caaaacattt gtatgactca 721 tacataaagt ccttcccgct gaccaaagca aaggcgaggg cgatcttgac aggaaagaca 781 acagacaaat caccattcgt tatctatgac atgaattcct taatgatggg agaagataaa 841 atcaagttca aacacatcac ccccctgcag gagcagagca aagaggtggc catccgcatc 901 tttcagggct gccagtttcg ctccgtggag gctgtgcagg agatcacaga gtatgccaaa 961 agcattcctg gttttgtaaa tcttgacttg aacgaccaag taactctcct caaatatgga 1021 gtccacgaga tcatttacac aatgctggcc tccttgatga ataaagatgg ggttctcata 1081 tccgagggcc aaggcttcat gacaagggag tttctaaaga gcctgcgaaa gccTTTTGGT 1141 GACTTTATGG AGCCCAAGTT TGAGTTTGCT GTGAAGTTCA ATGCACTGGA ATTAGATGAC 1201 AGCGACTTGG CAATATTTAT TGCTGTCATT ATTCTCAGTG GAGACCGCCC AGGTTTGCTG 1261 AATGTGAAGC CCATTGAAGA CATTCAAGAC AACCTGCTAC AAGCCCTGGA GCTCCAGCTG 1321 AAGCTGAACC ACCCTGAGTC CTCACAGCTG TTTGCCAAGC TGCTCCAGAA AATGACAGAC 1381 CTCAGACAGA TTGTCACGGA ACACGTGCAG CTACTGCAGG TGATCAAGAA GACGGAGACA 1441 GACATGAGTC TTCACCCGCT CCTGCAGGAG ATCTACAAGG ACTTGTACTA GGCTAGCGAC 1501 CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1561 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1621 TATATATCTT GTGGAAAGGA CGAGGCTGTC CTGGAAACAG ACATATGACG CGTTAAGTCg 1681 acaatcaacc tctggattac aaaatttgtg aaagatt