Construct: ORF TRCN0000488905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020232.1_s317c1
- DNA Barcode:
- TCATTGATAGAAAGAGTCCTGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKG1 (5592)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488905
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_006258.4 | 100% | 100% | |
| 2 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_001098512.3 | 91.8% | 88.7% | (many diffs) |
| 3 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016412.1 | 85.9% | 85.1% | (many diffs) |
| 4 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016413.1 | 85.2% | 84.8% | 0_1ins277;4_5insGACCCTGCCCTTCTACCC;8_9insAGCCCACA |
| 5 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_011539952.2 | 56.9% | 54% | (many diffs) |
| 6 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_024448078.1 | 41.2% | 41.2% | 0_1ins1209 |
| 7 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_011160.3 | 93.1% | 99.5% | (many diffs) |
| 8 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_001013833.3 | 85.7% | 88.3% | (many diffs) |
| 9 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | XM_006526768.3 | 85.7% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2130
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcacc ttgcgggatt tacagtacgc gctccaggag aagatcgagg 121 agctgaggca gcgggatgct ctcatcgacg agctggagct ggagttggat cagaaggacg 181 aactgatcca gaagctgcag aacgagctgg acaagtaccg ctcggtgatc cgaccagcca 241 cccagcaggc gcagaagcag agcgcgagca ccttgcaggg cgagccgcgc accaagcggc 301 aggcgatctc cgccgagccc accgccttcg acatccagga tctcagccat gtgaccctgc 361 ccttctaccc caagagccca cagtccaagg atcttataaa ggaagctatc cttgacaatg 421 actttatgaa gaacttggag ctgtcgcaga tccaggagat tgtggattgt atgtacccgg 481 tggagtatgg caaggacagt tgcatcatca aagaaggaga cgtggggtca ctggtgtatg 541 tcatggaaga tggtaaggtt gaagttacaa aagaaggtgt gaagttgtgt accatgggtc 601 caggaaaagt gtttggggaa ttggctattc tttacaactg tacccggaca gcgaccgtca 661 agactcttgt aaatgtaaaa ctctgggcca ttgatcgaca atgttttcaa acaataatga 721 tgaggacagg actcatcaag cataccgagt atatggaatt tttaaaaagc gttccaacat 781 tccagagcct tcctgaagag atcctcagca agcttgctga tgtccttgaa gagacccact 841 atgaaaatgg agaatatatt atcaggcaag gtgcaagagg ggacaccttc tttatcatca 901 gcaaaggaac ggtaaatgtc actcgtgaag actcaccgag tgaagaccca gtctttctta 961 gaactttagg aaaaggagac tggtttggag agaaagcctt gcagggggaa gatgtgagaa 1021 cagcaaacgt aattgctgca gaagctgtaa cctgccttgt gattgacaga gactctttta 1081 aacatttgat tggagggctg gatgatgttt ctaataaagc atatgaagat gcagaagcta 1141 aagcaaaata tgaagctgaa gcggctttct tcgccaacct gaagctgtct gatttcaaca 1201 tcattgatac ccttggagtt ggaggtttcg gacgagtaga actggtccag ttgaaaagtg 1261 aagaatccaa aacgtttgca atgaagattc tcaagaaacg tcacattgtg gacacaagac 1321 agcaggagca catccgctca gagaagcaga tcatgcaggg ggctcattcc gatttcatag 1381 tgagactgta cagaacattt aaggacagca aatatttgta tatgttgatg gaagcttgtc 1441 taggtggaga gctctggacc attctcaggg atagaggttc gtttgaagat tctacaacca 1501 gattttacac agcatgtgtg gtagaagctt ttgcctatct gcattccaaa ggaatcattt 1561 acagggacct caagccagaa aatctcatcc tagatcaccg aggttatgcc aaactggttg 1621 attttggctt tgcaaagaaa ataggatttg gaaagaaaac atggactttt tgtgggactc 1681 cagagtatgt agccccagag atcatcctga acaaaggcca tgacatttca gccgactact 1741 ggtcactggg aatcctaatg tatgaactcc tgactggcag cccaccTTTC TCAGGCCCAG 1801 ATCCTATGAA AACCTATAAC ATCATATTGA GGGGGATTGA CATGATAGAA TTTCCAAAGA 1861 AGATTGCCAA AAATGCTGCT AATTTAATTA AAAAACTATG CAGGGACAAT CCATCAGAAA 1921 GATTAGGGAA TTTGAAAAAT GGAGTAAAAG ACATTCAAAA GCACAAATGG TTTGAGGGCT 1981 TTAACTGGGA AGGCTTAAGA AAAGGTACCT TGACACCTCC TATAATACCA AGTGTTGCAT 2041 CACCCACAGA CACAAGTAAT TTTGACAGTT TCCCTGAGGA CAACGATGAA CCACCACCTG 2101 ATGACAACTC AGGATGGGAT ATAGACTTCT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 2161 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2221 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 2281 ATTGATAGAA AGAGTCCTGC ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 2341 ttgtgaaaga tt