Construct: ORF TRCN0000488905

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020232.1_s317c1
DNA Barcode:
TCATTGATAGAAAGAGTCCTGCAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PRKG1 (5592)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488905
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5592 PRKG1 protein kinase cGMP-depende... NM_006258.4 100% 100%
2 human 5592 PRKG1 protein kinase cGMP-depende... NM_001098512.3 91.8% 88.7% (many diffs)
3 human 5592 PRKG1 protein kinase cGMP-depende... XM_017016412.1 85.9% 85.1% (many diffs)
4 human 5592 PRKG1 protein kinase cGMP-depende... XM_017016413.1 85.2% 84.8% 0_1ins277;4_5insGACCCTGCCCTTCTACCC;8_9insAGCCCACA
5 human 5592 PRKG1 protein kinase cGMP-depende... XM_011539952.2 56.9% 54% (many diffs)
6 human 5592 PRKG1 protein kinase cGMP-depende... XM_024448078.1 41.2% 41.2% 0_1ins1209
7 mouse 19091 Prkg1 protein kinase, cGMP-depend... NM_011160.3 93.1% 99.5% (many diffs)
8 mouse 19091 Prkg1 protein kinase, cGMP-depend... NM_001013833.3 85.7% 88.3% (many diffs)
9 mouse 19091 Prkg1 protein kinase, cGMP-depend... XM_006526768.3 85.7% 88.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2130
ORF length:
2058
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgggcacc ttgcgggatt tacagtacgc gctccaggag aagatcgagg
121agctgaggca gcgggatgct ctcatcgacg agctggagct ggagttggat cagaaggacg
181aactgatcca gaagctgcag aacgagctgg acaagtaccg ctcggtgatc cgaccagcca
241cccagcaggc gcagaagcag agcgcgagca ccttgcaggg cgagccgcgc accaagcggc
301aggcgatctc cgccgagccc accgccttcg acatccagga tctcagccat gtgaccctgc
361ccttctaccc caagagccca cagtccaagg atcttataaa ggaagctatc cttgacaatg
421actttatgaa gaacttggag ctgtcgcaga tccaggagat tgtggattgt atgtacccgg
481tggagtatgg caaggacagt tgcatcatca aagaaggaga cgtggggtca ctggtgtatg
541tcatggaaga tggtaaggtt gaagttacaa aagaaggtgt gaagttgtgt accatgggtc
601caggaaaagt gtttggggaa ttggctattc tttacaactg tacccggaca gcgaccgtca
661agactcttgt aaatgtaaaa ctctgggcca ttgatcgaca atgttttcaa acaataatga
721tgaggacagg actcatcaag cataccgagt atatggaatt tttaaaaagc gttccaacat
781tccagagcct tcctgaagag atcctcagca agcttgctga tgtccttgaa gagacccact
841atgaaaatgg agaatatatt atcaggcaag gtgcaagagg ggacaccttc tttatcatca
901gcaaaggaac ggtaaatgtc actcgtgaag actcaccgag tgaagaccca gtctttctta
961gaactttagg aaaaggagac tggtttggag agaaagcctt gcagggggaa gatgtgagaa
1021cagcaaacgt aattgctgca gaagctgtaa cctgccttgt gattgacaga gactctttta
1081aacatttgat tggagggctg gatgatgttt ctaataaagc atatgaagat gcagaagcta
1141aagcaaaata tgaagctgaa gcggctttct tcgccaacct gaagctgtct gatttcaaca
1201tcattgatac ccttggagtt ggaggtttcg gacgagtaga actggtccag ttgaaaagtg
1261aagaatccaa aacgtttgca atgaagattc tcaagaaacg tcacattgtg gacacaagac
1321agcaggagca catccgctca gagaagcaga tcatgcaggg ggctcattcc gatttcatag
1381tgagactgta cagaacattt aaggacagca aatatttgta tatgttgatg gaagcttgtc
1441taggtggaga gctctggacc attctcaggg atagaggttc gtttgaagat tctacaacca
1501gattttacac agcatgtgtg gtagaagctt ttgcctatct gcattccaaa ggaatcattt
1561acagggacct caagccagaa aatctcatcc tagatcaccg aggttatgcc aaactggttg
1621attttggctt tgcaaagaaa ataggatttg gaaagaaaac atggactttt tgtgggactc
1681cagagtatgt agccccagag atcatcctga acaaaggcca tgacatttca gccgactact
1741ggtcactggg aatcctaatg tatgaactcc tgactggcag cccaccTTTC TCAGGCCCAG
1801ATCCTATGAA AACCTATAAC ATCATATTGA GGGGGATTGA CATGATAGAA TTTCCAAAGA
1861AGATTGCCAA AAATGCTGCT AATTTAATTA AAAAACTATG CAGGGACAAT CCATCAGAAA
1921GATTAGGGAA TTTGAAAAAT GGAGTAAAAG ACATTCAAAA GCACAAATGG TTTGAGGGCT
1981TTAACTGGGA AGGCTTAAGA AAAGGTACCT TGACACCTCC TATAATACCA AGTGTTGCAT
2041CACCCACAGA CACAAGTAAT TTTGACAGTT TCCCTGAGGA CAACGATGAA CCACCACCTG
2101ATGACAACTC AGGATGGGAT ATAGACTTCT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG
2161ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2221GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC
2281ATTGATAGAA AGAGTCCTGC ACACGCGTTA AGTCgacaat caacctctgg attacaaaat
2341ttgtgaaaga tt

Download FASTA (ORF) (Full)

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