Construct: ORF TRCN0000489003

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020554.1_s317c1
DNA Barcode:
GTAAATACAATCATGACTCATCGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PRKCZ (5590)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489003
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5590 PRKCZ protein kinase C zeta NM_002744.6 100% 100%
2 human 5590 PRKCZ protein kinase C zeta XM_017001790.1 98.6% 98.6% 974_997del
3 human 5590 PRKCZ protein kinase C zeta XM_011541773.1 91.2% 86.1% (many diffs)
4 human 5590 PRKCZ protein kinase C zeta XM_017001789.1 90.1% 85% (many diffs)
5 human 5590 PRKCZ protein kinase C zeta NM_001242874.2 82.2% 81.4% (many diffs)
6 human 5590 PRKCZ protein kinase C zeta XM_011541774.1 74.1% 68.5% (many diffs)
7 human 5590 PRKCZ protein kinase C zeta XR_002957131.1 73.8% 1_157del;1732_1814del;2017_2405del
8 human 5590 PRKCZ protein kinase C zeta XR_002957130.1 73.1% (many diffs)
9 human 5590 PRKCZ protein kinase C zeta NM_001033581.2 69% 69% 0_1ins549
10 human 5590 PRKCZ protein kinase C zeta NM_001033582.2 69% 69% 0_1ins549
11 human 5590 PRKCZ protein kinase C zeta XM_017001801.1 68.4% 68.4% 0_1ins561
12 human 5590 PRKCZ protein kinase C zeta NM_001350803.1 68.1% 68.1% 0_1ins549;425_448del
13 human 5590 PRKCZ protein kinase C zeta NM_001350804.1 68.1% 68.1% 0_1ins549;425_448del
14 human 5590 PRKCZ protein kinase C zeta NR_146911.1 66.6% (many diffs)
15 human 5590 PRKCZ protein kinase C zeta XM_011541775.3 66.5% 56.3% (many diffs)
16 human 5590 PRKCZ protein kinase C zeta XM_011541776.1 61.5% 56.8% (many diffs)
17 human 5590 PRKCZ protein kinase C zeta XM_024448396.1 61.5% 56.8% (many diffs)
18 human 5590 PRKCZ protein kinase C zeta XM_017001800.1 60.9% 56.2% (many diffs)
19 human 5590 PRKCZ protein kinase C zeta XM_017001792.1 60.7% 56.1% (many diffs)
20 human 5590 PRKCZ protein kinase C zeta XM_017001793.1 60.7% 56.1% (many diffs)
21 human 5590 PRKCZ protein kinase C zeta XM_024448391.1 60.7% 56.1% (many diffs)
22 human 5590 PRKCZ protein kinase C zeta XM_017001797.1 60.1% 55.5% (many diffs)
23 human 5590 PRKCZ protein kinase C zeta NM_001350805.1 59.9% 59.9% 0_1ins711
24 human 5590 PRKCZ protein kinase C zeta NM_001350806.1 59.9% 59.9% 0_1ins711
25 human 5590 PRKCZ protein kinase C zeta XM_017001803.2 58.1% 56.2% (many diffs)
26 human 5590 PRKCZ protein kinase C zeta XR_001737315.2 57.6% (many diffs)
27 human 5590 PRKCZ protein kinase C zeta XM_017001802.1 52.8% 48.2% (many diffs)
28 human 5590 PRKCZ protein kinase C zeta XM_024448397.1 52.1% 47.6% (many diffs)
29 mouse 18762 Prkcz protein kinase C, zeta NM_008860.3 88.8% 95.6% (many diffs)
30 mouse 18762 Prkcz protein kinase C, zeta XM_011250207.2 78% 84.6% (many diffs)
31 mouse 18762 Prkcz protein kinase C, zeta XM_006538655.3 72.7% 79.3% (many diffs)
32 mouse 18762 Prkcz protein kinase C, zeta NM_001039079.2 61% 67.7% (many diffs)
33 mouse 18762 Prkcz protein kinase C, zeta XM_006538656.2 61% 67.7% (many diffs)
34 mouse 18762 Prkcz protein kinase C, zeta XM_017320042.1 61% 67.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1848
ORF length:
1776
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcccagc aggaccggcc ccaagatgga agggagcggc ggccgcgtcc
121gcctcaaggc gcattacggg ggggacatct tcatcaccag cgtggacgcc gccacgacct
181tcgaggagct ctgtgaggaa gtgagagaca tgtgtcgtct gcaccagcag cacccgctca
241ccctcaagtg ggtggacagc gaaggtgacc cttgcacggt gtcctcccag atggagctgg
301aagaggcttt ccgcctggcc cgtcagtgca gggatgaagg cctcatcatt catgttttcc
361cgagcacccc tgagcagcct ggcctgccat gtccgggaga agacaaatct atctaccgcc
421ggggagccag aagatggagg aagctgtacc gtgccaacgg ccacctcttc caagccaagc
481gctttaacag gagagcgtac tgcggtcagt gcagcgagag gatatggggc ctcgcgaggc
541aaggctacag gtgcatcaac tgcaaactgc tggtccataa gcgctgccac ggcctcgtcc
601cgctgacctg caggaagcat atggattctg tcatgccttc ccaagagcct ccagtagacg
661acaagaacga ggacgccgac cttccttccg aggagacaga tggaattgct tacatttcct
721catcccggaa gcatgacagc attaaagacg actcggagga ccttaagcca gttatcgatg
781ggatggatgg aatcaaaatc tctcaggggc ttgggctgca ggactttgac ctaatcagag
841tcatcgggcg cgggagctac gccaaggttc tcctggtgcg gttgaagaag aatgaccaaa
901tttacgccat gaaagtggtg aagaaagagc tggtgcatga tgacgaggat attgactggg
961tacagacaga gaagcacgtg tttgagcagg catccagcaa ccccttcctg gtcggattac
1021actcctgctt ccagacgaca agtcggttgt tcctggtcat tgagtacgtc aacggcgggg
1081acctgatgtt ccacatgcag aggcagagga agctccctga ggagcacgcc aggttctacg
1141cggccgagat ctgcatcgcc ctcaacttcc tgcacgagag ggggatcatc tacagggacc
1201tgaagctgga caacgtcctc ctggatgcgg acgggcacat caagctcaca gactacggca
1261tgtgcaagga aggcctgggc cctggtgaca caacgagcac tttctgcgga accccgaatt
1321acatcgcccc cgaaatcctg cggggagagg agtacgggtt cagcgtggac tggtgggcgc
1381tgggagtcct catgtttgag atgatggccg ggcgctcccc gttcgacatc atcaccgaca
1441acccggacat gaacacagag gactaccttt tccaagtgat cctggagaag cccatccgga
1501TCCCCCGGTT CCTGTCCGTC AAAGCCTCCC ATGTTTTAAA AGGATTTTTA AATAAGGACC
1561CCAAAGAGAG GCTCGGCTGC CGGCCACAGA CTGGATTTTC TGACATCAAG TCCCACGCGT
1621TCTTCCGCAG CATAGACTGG GACTTGCTGG AGAAGAAGCA GGCGCTCCCT CCATTCCAGC
1681CACAGATCAC AGACGACTAC GGTCTGGACA ACTTTGACAC ACAGTTCACC AGCGAGCCCG
1741TGCAGCTGAC CCCAGACGAT GAGGATGCCA TAAAGAGGAT CGACCAGTCA GAGTTCGAAG
1801GCTTTGAGTA TATCAACCCA TTATTGCTGT CCACCGAGGA GTCGGTGTGA AAGCTTGACC
1861CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1921TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1981ATATATCTTG TGGAAAGGAC GAGTAAATAC AATCATGACT CATCGGACGC GTTAAGTCga
2041caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)