Construct: ORF TRCN0000489132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020032.1_s317c1
- DNA Barcode:
- AGAAAATTTGATATAATTGCCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FER (2241)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489132
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2241 | FER | FER tyrosine kinase | NM_005246.4 | 99.9% | 99.8% | 2466_2467insG |
| 2 | human | 2241 | FER | FER tyrosine kinase | XM_011543271.3 | 99.9% | 99.8% | 2466_2467insG |
| 3 | human | 2241 | FER | FER tyrosine kinase | XM_017009230.2 | 99.9% | 99.8% | 2466_2467insG |
| 4 | human | 2241 | FER | FER tyrosine kinase | XM_017009231.2 | 99.9% | 99.8% | 2466_2467insG |
| 5 | human | 2241 | FER | FER tyrosine kinase | XM_011543266.2 | 98.1% | 98% | 2048_2092del;2511_2512insG |
| 6 | human | 2241 | FER | FER tyrosine kinase | XM_011543267.2 | 98.1% | 98% | 2048_2092del;2511_2512insG |
| 7 | human | 2241 | FER | FER tyrosine kinase | XM_011543269.2 | 98.1% | 98% | 2048_2092del;2511_2512insG |
| 8 | human | 2241 | FER | FER tyrosine kinase | XM_011543270.3 | 98.1% | 98% | 2048_2092del;2511_2512insG |
| 9 | human | 2241 | FER | FER tyrosine kinase | XM_017009229.2 | 98.1% | 98% | 2048_2092del;2511_2512insG |
| 10 | human | 2241 | FER | FER tyrosine kinase | XM_017009232.2 | 90.4% | 90.2% | (many diffs) |
| 11 | human | 2241 | FER | FER tyrosine kinase | XM_011543272.2 | 90% | 89.9% | 1329_1330ins204;1844_1888del;2307_2308insG |
| 12 | human | 2241 | FER | FER tyrosine kinase | XM_017009233.2 | 89.4% | 89.3% | 0_1ins219;1829_1873del;2292_2293insG |
| 13 | human | 2241 | FER | FER tyrosine kinase | NM_001308028.2 | 78.6% | 78.6% | 0_1ins525;1941_1942insG |
| 14 | human | 2241 | FER | FER tyrosine kinase | XR_948244.2 | 78.2% | (many diffs) | |
| 15 | human | 2241 | FER | FER tyrosine kinase | XM_017009234.1 | 67.9% | 66.9% | (many diffs) |
| 16 | human | 2241 | FER | FER tyrosine kinase | XM_017009235.1 | 62.8% | 60.6% | 1468_1469ins65;1551_1552ins851 |
| 17 | human | 2241 | FER | FER tyrosine kinase | XR_001742041.1 | 62.5% | 1_237del;1705_1706ins65;1929_1930ins710 | |
| 18 | human | 2241 | FER | FER tyrosine kinase | XR_002956153.1 | 55.6% | 1_237del;1473_1642del;2007_2008ins867 | |
| 19 | human | 2241 | FER | FER tyrosine kinase | NM_001308031.1 | 53.2% | 50.7% | (many diffs) |
| 20 | human | 2241 | FER | FER tyrosine kinase | XM_011543276.2 | 52.2% | 49.8% | (many diffs) |
| 21 | human | 2241 | FER | FER tyrosine kinase | XM_011543278.1 | 50.9% | 50.3% | (many diffs) |
| 22 | human | 2241 | FER | FER tyrosine kinase | NR_146155.2 | 20.5% | 1_105del;1341_1510del;2742_12010delinsG | |
| 23 | human | 2241 | FER | FER tyrosine kinase | NM_001308038.2 | 19.7% | 19.5% | 482T>G;486_487insGAAACTGAA;489_490ins1969 |
| 24 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_006523643.4 | 88.3% | 92.9% | (many diffs) |
| 25 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_011246293.3 | 88.3% | 92.9% | (many diffs) |
| 26 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_030249496.1 | 88.3% | 92.9% | (many diffs) |
| 27 | mouse | 14158 | Fer | fer (fms/fps related) prote... | NM_001037997.3 | 88.2% | 92.8% | (many diffs) |
| 28 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_006523640.4 | 88.2% | 92.8% | (many diffs) |
| 29 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_006523641.4 | 88.2% | 92.8% | (many diffs) |
| 30 | mouse | 14158 | Fer | fer (fms/fps related) prote... | NM_001286415.1 | 81.7% | 86% | (many diffs) |
| 31 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_006523645.4 | 80.1% | 84.3% | (many diffs) |
| 32 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_017317242.2 | 80.1% | 84.3% | (many diffs) |
| 33 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XR_003952087.1 | 69% | (many diffs) | |
| 34 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XR_003952086.1 | 68.3% | (many diffs) | |
| 35 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XR_003952084.1 | 68.2% | (many diffs) | |
| 36 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XR_003952085.1 | 65.2% | (many diffs) | |
| 37 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XR_385280.4 | 65.2% | (many diffs) | |
| 38 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XR_385281.4 | 46.6% | (many diffs) | |
| 39 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_006523650.4 | 45.8% | 47.8% | (many diffs) |
| 40 | mouse | 14158 | Fer | fer (fms/fps related) prote... | XM_017317244.1 | 45.2% | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2538
- ORF length:
- 2469
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggggtttggg agtgacctga agaattcaca tgaagcagtg ttaaaattgc 121 aagactggga attacggtta ctggaaacag taaagaaatt tatggccctg agaataaaaa 181 gtgataaaga atatgcatct actttacaga acctttgtaa tcaagttgat aaggaaagta 241 ctgtccaaat gaattatgtc agcaacgtat ccaagtcttg gctacttatg attcagcaga 301 cagaacaact tagtaggata atgaagacac atgcagagga cttgaactct ggacctttac 361 acaggctcac catgatgatt aaggacaagc agcaggtgaa gaaaagttac ataggtgttc 421 atcagcagat agaggcagag atgatcaagg ttaccaaaac agaattggag aagttaaaat 481 gcagctatag acaattaata aaagaaatga attctgccaa agagaaatat aaagaagctt 541 tagctaaagg gaaggaaact gaaaaggcca aggaacgata cgacaaagcc acaatgaaac 601 ttcatatgtt gcacaatcag tatgtattgg cgttgaaagg ggcacagctc catcagaatc 661 agtattatga tatcacactt cccctgcttc tggactcctt acaaaagatg caagaagaaa 721 tgataaaagc actcaaaggt atatttgatg aatacagcca gataaccagt cttgtcacag 781 aggaaatagt gaatgtccat aaagagattc aaatgtcggt tgaacagata gatcctagta 841 cagaatacaa taatttcata gatgttcaca gaacaacggc tgctaaagaa caagaaatag 901 agtttgatac ttccttactg gaagaaaatg aaaatcttca ggcaaatgag atcatgtgga 961 ataacttaac agcagaaagt ttgcaagtaa tgttgaaaac gttagcggaa gaacttatgc 1021 aaacacagca gatgctttta aacaaggagg aggctgtttt ggagttagag aagagaattg 1081 aagaatcttc tgaaacttgt gagaagaagt ctgatattgt gcttctgcta agccaaaaac 1141 aggcacttga agaactgaaa cagtcagtcc agcagctgag atgcactgaa gcaaagtttt 1201 cagcacagaa ggaattacta gagcaaaaag tgcaagaaaa tgatgggaaa gagccacctc 1261 cagtagtaaa ttatgaagaa gatgcacgat cagttacatc tatggaaaga aaggagaggc 1321 tatccaaatt tgaatctatt cgtcattcaa ttgctggaat aattaggtct ccaaaatctg 1381 cactgggctc ttcagcactt tctgatatga tctccatcag tgagaagcct ttggcagaac 1441 aggactggta ccatggtgca attcccagaa tagaagctca agaactgtta aaaaaacaag 1501 gagacttttt ggtgcgagag agtcatggga aacctggtga atatgtcctt tctgtatatt 1561 ctgatggaca gaggagacat tttatcatac aatatgttga taacatgtat cgattcgagg 1621 gcactgggtt ttcaaacatt cctcaactta tagatcatca ctatacaaca aaacaggtca 1681 tcactaagaa atcaggtgta gttctgctga atcctattcc taaggacaag aaatggattc 1741 tcagtcatga agatgtcata ttgggagaat tactgggcaa gggaaatttt ggtgaagtat 1801 ataagggcac attaaaggat aaaacttctg ttgctgttaa aacatgtaaa gaagatcttc 1861 ctcaggaatt gaaaataaaa tttttacaag aagccaaaat tctcaagcaa tatgatcatc 1921 ccaatattgt caaacttata ggagtttgca cacaaagaca gcctgtctac atcattatgg 1981 aactggtttc aggaggtgat ttcctcacct ttctgagaag gaagaaggat gaactaaaac 2041 tcaaacagtt agtgaaattt tcattagacg ctgctgctgg tatgttgtat ctcgagagta 2101 aaaactgtat acacagggac cttgctgcaa gaaactgcct ggtaggtgaa aataatgttc 2161 tgaaaatcag tgactttgga atgtctcgtc aagaggatgg tggagtgtat tcaTCTTCTG 2221 GCTTAAAGCA GATTCCCATT AAATGGACAG CACCGGAAGC TCTTAATTAT GGGAGATACA 2281 GTTCAGAGAG TGACGTGTGG AGCTTTGGCA TCCTTCTCTG GGAGACCTTC AGCTTAGGGG 2341 TTTGTCCGTA CCCTGGAATG ACAAATCAGC AAGCAAGAGA GCAAGTAGAA AGAGGATACC 2401 GGATGTCAGC TCCCCAGCAC TGTCCAGAGG ATATTTCCAA AATCATGATG AAGTGTTGGG 2461 ATTATAAACC TGAAAATCGC CCTAAGTTCA GTGAACTTCA GAAAGAGCTC ACTATCATCA 2521 AGAGAAAACT CACAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2581 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2641 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGAAAATTTG ATATAATTGC 2701 CAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt