Construct: ORF TRCN0000489226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020039.1_s317c1
- DNA Barcode:
- CCATCTCCACAAAATCGCTAAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRM5 (2915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489226
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2915 | GRM5 | glutamate metabotropic rece... | NM_000842.4 | 99.9% | 99.9% | 3279A>C;3540_3541insG |
| 2 | human | 2915 | GRM5 | glutamate metabotropic rece... | XM_017017627.2 | 99.9% | 99.9% | 3279A>C;3540_3541insG |
| 3 | human | 2915 | GRM5 | glutamate metabotropic rece... | NM_001143831.2 | 97.3% | 97.2% | 2629_2724del;3375A>C;3636_3637insG |
| 4 | human | 2915 | GRM5 | glutamate metabotropic rece... | XM_006718828.4 | 97.3% | 97.2% | 2629_2724del;3375A>C;3636_3637insG |
| 5 | human | 2915 | GRM5 | glutamate metabotropic rece... | XM_011542792.1 | 97.3% | 97.2% | 2629_2724del;3375A>C;3636_3637insG |
| 6 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | NM_001081414.2 | 88.6% | 95.2% | (many diffs) |
| 7 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | NM_001143834.1 | 86.2% | 92.7% | (many diffs) |
| 8 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | XM_011241643.2 | 86.2% | 92.7% | (many diffs) |
| 9 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | XM_006507184.3 | 74.3% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3612
- ORF length:
- 3543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtccttctg ttgatcctgt cagtcttact tttgaaagaa gatgtccgtg 121 ggagtgcaca gtccagtgag aggagggtgg tggctcacat gccgggtgac atcattattg 181 gagctctctt ttctgttcat caccagccta ctgtggacaa agttcatgag aggaagtgtg 241 gggcggtccg tgaacagtat ggcattcaga gagtggaggc catgctgcat accctggaaa 301 ggatcaattc agaccccaca ctcttgccca acatcacact gggctgtgag ataagggact 361 cctgctggca ttcggctgtg gccctagagc agagcattga gttcataaga gattccctca 421 tttcttcaga agaggaagaa ggcttggtac gctgtgtgga tggctcctcc tcttccttcc 481 gctccaagaa gcccatagta ggggtcattg ggcctggctc cagttctgta gccattcagg 541 tccagaattt gctccagctt ttcaacatac ctcagattgc ttactcagca accagcatgg 601 atctgagtga caagactctg ttcaaatatt tcatgagggt tgtgccttca gatgctcagc 661 aggcaagggc catggtggac atagtgaaga ggtacaactg gacctatgta tcagccgtgc 721 acacagaagg caactatgga gaaagtggga tggaagcctt caaagatatg tcagcgaagg 781 aagggatttg catcgcccac tcttacaaaa tctacagtaa tgcaggggag cagagctttg 841 ataagctgct gaagaagctc acaagtcact tgcccaaggc ccgggtggtg gcctgcttct 901 gtgagggcat gacggtgaga ggtctgctga tggccatgag gcgcctgggt ctagcgggag 961 aatttctgct tctgggcagt gatggctggg ctgacaggta tgatgtgaca gatggatatc 1021 agcgagaagc tgttggtggc atcacaatca agctccaatc tcccgatgtc aagtggtttg 1081 atgattatta tctgaagctc cggccagaaa caaaccaccg aaacccttgg tttcaagaat 1141 tttggcagca tcgttttcag tgccgactgg aagggtttcc acaggagaac agcaaataca 1201 acaagacttg caatagttct ctgactctga aaacacatca tgttcaggat tccaaaatgg 1261 gatttgtgat caacgccatc tattcgatgg cctatgggct ccacaacatg cagatgtccc 1321 tctgcccagg ctatgcagga ctctgtgatg ccatgaagcc aattgatgga cggaaacttt 1381 tggagtccct gatgaaaacc aattttactg gggtttctgg agatacgatc ctattcgatg 1441 agaatggaga ctctccagga aggtatgaaa taatgaattt caaggaaatg ggaaaagatt 1501 actttgatta tatcaacgtt ggaagttggg acaatggaga attaaaaatg gatgatgatg 1561 aagtatggtc caagaaaagc aacatcatca gatctgtgtg cagtgaacca tgtgagaaag 1621 gccagatcaa ggtgatccga aagggagaag tcagctgttg ttggacctgt acaccttgta 1681 aggagaatga gtatgtcttt gatgagtaca catgcaaggc atgccaactg gggtcttggc 1741 ccactgatga tctcacaggt tgtgacttga tcccagtaca gtatcttcga tggggtgacc 1801 ctgaacccat tgcagctgtg gtgtttgcct gccttggcct cctggccacc ctgtttgtta 1861 ctgtagtctt catcatttac cgtgatacac cagtagtcaa gtcctcaagc agggaactct 1921 gctacattat ccttgctggc atctgcctgg gctacttatg taccttctgc ctcattgcga 1981 agcccaaaca gatttactgc taccttcaga gaattggcat tggtctctcc ccagccatga 2041 gctactcagc ccttgtaaca aagaccaacc gtattgcaag gatcctggct ggcagcaaga 2101 agaagatctg taccaaaaag cccagattca tgagtgcctg tgcccagcta gtgattgctt 2161 tcattctcat atgcatccag ttgggcatca tcgttgccct ctttataatg gagcctcctg 2221 acataatgca tgactaccca agcattcgag aagtctacct gatctgtaac accaccaacc 2281 taggagttgt cactccactt ggatacaatg gattgttgat tttgagctgc accttctatg 2341 cgttcaagac cagaaatgtt ccagctaact tcaacgaggc caagtatatc gccttcacaa 2401 tgtacacgac ctgcattata tggctagctt ttgtgccaat ctactttggc agcaactaca 2461 aaatcatcac catgtgtttc tcggtcagcc tcagtgccac agtggcccta ggctgcatgt 2521 ttgtgccgaa ggtgtacatc atcctggcca aaccagagag aaacgtgcgc agcgccttca 2581 ccacatctac cgtggtgcgc atgcatgtag gggatggcaa gtcatcctcc gcagccagca 2641 gatccagcag cctagtcaac ctgtggaaga gaaggggctc ctctggggaa accttaagtt 2701 ccaatggaaa atccgtcacg tgggcccaga atgagaagag cagccggggg cagcacctgt 2761 ggcagcgcct gtccatccac atcaacaaga aagaaaaccc caaccaaacg gccgtcatca 2821 agcccttccc caagagcacg gagagccgtg gcctgggcgc tggcgctggc gcaggcggga 2881 gcgctggggg cgtgggggcc acgggcggtg cgggctgcgc aggcgccggc ccaggcgggc 2941 ccgagtcccc agacgccggc cccaaggcgc tgtatgatgt ggccgaggct gaggagcact 3001 tcccggcgcc cgcgcggccg cgctcaccgt cgcccatcag cacgctgagc caccgcgcgg 3061 gctcggccag ccgcacggac gacgatgtgc cgtcgctgca ctcggagcct gtggcgcgca 3121 gcagctcctc gcagggctcc ctcatggagc agatcagcag tgtggtcacc cgcttcacgg 3181 ccaacatcag cgagctcaac tccatgatgc tgtccaccgc ggcccccagc cccggcgtcg 3241 gcgccccgct ctgctcgtcc tacctgaTCC CCAAAGAGAT CCAGTTGCCC ACGACCATGA 3301 CGACCTTTGC CGAAATCCAG CCTCTGCCGG CCATCGAAGT CACGGGCGGC GCGCAGCCCG 3361 CGGCAGGGGC GCAGGCGGCT GGGGACGCGG CCCGGGAGAG CCCCGCGGCC GGTCCCGAGG 3421 CTGCGGCCGC CAAGCCAGAC CTGGAGGAGC TGGTGGCTCT CACCCCGCCG TCCCCCTTCA 3481 GAGACTCGGT GGACTCGGGG AGCACAACCC CCAACTCGCC AGTGTCCGAG TCGGCCCTCT 3541 GTATCCCGTC GTCTCCCAAA TATGACACTC TTATCATAAG AGATTACACT CAGAGCTCCT 3601 CGTCGTTGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCATCT CCACAAAATC GCTAAGCAAC 3781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt