Construct: ORF TRCN0000489226

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020039.1_s317c1
DNA Barcode:
CCATCTCCACAAAATCGCTAAGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRM5 (2915)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489226
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2915 GRM5 glutamate metabotropic rece... NM_000842.4 99.9% 99.9% 3279A>C;3540_3541insG
2 human 2915 GRM5 glutamate metabotropic rece... XM_017017627.2 99.9% 99.9% 3279A>C;3540_3541insG
3 human 2915 GRM5 glutamate metabotropic rece... NM_001143831.2 97.3% 97.2% 2629_2724del;3375A>C;3636_3637insG
4 human 2915 GRM5 glutamate metabotropic rece... XM_006718828.4 97.3% 97.2% 2629_2724del;3375A>C;3636_3637insG
5 human 2915 GRM5 glutamate metabotropic rece... XM_011542792.1 97.3% 97.2% 2629_2724del;3375A>C;3636_3637insG
6 mouse 108071 Grm5 glutamate receptor, metabot... NM_001081414.2 88.6% 95.2% (many diffs)
7 mouse 108071 Grm5 glutamate receptor, metabot... NM_001143834.1 86.2% 92.7% (many diffs)
8 mouse 108071 Grm5 glutamate receptor, metabot... XM_011241643.2 86.2% 92.7% (many diffs)
9 mouse 108071 Grm5 glutamate receptor, metabot... XM_006507184.3 74.3% 75.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3612
ORF length:
3543
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggtccttctg ttgatcctgt cagtcttact tttgaaagaa gatgtccgtg
121ggagtgcaca gtccagtgag aggagggtgg tggctcacat gccgggtgac atcattattg
181gagctctctt ttctgttcat caccagccta ctgtggacaa agttcatgag aggaagtgtg
241gggcggtccg tgaacagtat ggcattcaga gagtggaggc catgctgcat accctggaaa
301ggatcaattc agaccccaca ctcttgccca acatcacact gggctgtgag ataagggact
361cctgctggca ttcggctgtg gccctagagc agagcattga gttcataaga gattccctca
421tttcttcaga agaggaagaa ggcttggtac gctgtgtgga tggctcctcc tcttccttcc
481gctccaagaa gcccatagta ggggtcattg ggcctggctc cagttctgta gccattcagg
541tccagaattt gctccagctt ttcaacatac ctcagattgc ttactcagca accagcatgg
601atctgagtga caagactctg ttcaaatatt tcatgagggt tgtgccttca gatgctcagc
661aggcaagggc catggtggac atagtgaaga ggtacaactg gacctatgta tcagccgtgc
721acacagaagg caactatgga gaaagtggga tggaagcctt caaagatatg tcagcgaagg
781aagggatttg catcgcccac tcttacaaaa tctacagtaa tgcaggggag cagagctttg
841ataagctgct gaagaagctc acaagtcact tgcccaaggc ccgggtggtg gcctgcttct
901gtgagggcat gacggtgaga ggtctgctga tggccatgag gcgcctgggt ctagcgggag
961aatttctgct tctgggcagt gatggctggg ctgacaggta tgatgtgaca gatggatatc
1021agcgagaagc tgttggtggc atcacaatca agctccaatc tcccgatgtc aagtggtttg
1081atgattatta tctgaagctc cggccagaaa caaaccaccg aaacccttgg tttcaagaat
1141tttggcagca tcgttttcag tgccgactgg aagggtttcc acaggagaac agcaaataca
1201acaagacttg caatagttct ctgactctga aaacacatca tgttcaggat tccaaaatgg
1261gatttgtgat caacgccatc tattcgatgg cctatgggct ccacaacatg cagatgtccc
1321tctgcccagg ctatgcagga ctctgtgatg ccatgaagcc aattgatgga cggaaacttt
1381tggagtccct gatgaaaacc aattttactg gggtttctgg agatacgatc ctattcgatg
1441agaatggaga ctctccagga aggtatgaaa taatgaattt caaggaaatg ggaaaagatt
1501actttgatta tatcaacgtt ggaagttggg acaatggaga attaaaaatg gatgatgatg
1561aagtatggtc caagaaaagc aacatcatca gatctgtgtg cagtgaacca tgtgagaaag
1621gccagatcaa ggtgatccga aagggagaag tcagctgttg ttggacctgt acaccttgta
1681aggagaatga gtatgtcttt gatgagtaca catgcaaggc atgccaactg gggtcttggc
1741ccactgatga tctcacaggt tgtgacttga tcccagtaca gtatcttcga tggggtgacc
1801ctgaacccat tgcagctgtg gtgtttgcct gccttggcct cctggccacc ctgtttgtta
1861ctgtagtctt catcatttac cgtgatacac cagtagtcaa gtcctcaagc agggaactct
1921gctacattat ccttgctggc atctgcctgg gctacttatg taccttctgc ctcattgcga
1981agcccaaaca gatttactgc taccttcaga gaattggcat tggtctctcc ccagccatga
2041gctactcagc ccttgtaaca aagaccaacc gtattgcaag gatcctggct ggcagcaaga
2101agaagatctg taccaaaaag cccagattca tgagtgcctg tgcccagcta gtgattgctt
2161tcattctcat atgcatccag ttgggcatca tcgttgccct ctttataatg gagcctcctg
2221acataatgca tgactaccca agcattcgag aagtctacct gatctgtaac accaccaacc
2281taggagttgt cactccactt ggatacaatg gattgttgat tttgagctgc accttctatg
2341cgttcaagac cagaaatgtt ccagctaact tcaacgaggc caagtatatc gccttcacaa
2401tgtacacgac ctgcattata tggctagctt ttgtgccaat ctactttggc agcaactaca
2461aaatcatcac catgtgtttc tcggtcagcc tcagtgccac agtggcccta ggctgcatgt
2521ttgtgccgaa ggtgtacatc atcctggcca aaccagagag aaacgtgcgc agcgccttca
2581ccacatctac cgtggtgcgc atgcatgtag gggatggcaa gtcatcctcc gcagccagca
2641gatccagcag cctagtcaac ctgtggaaga gaaggggctc ctctggggaa accttaagtt
2701ccaatggaaa atccgtcacg tgggcccaga atgagaagag cagccggggg cagcacctgt
2761ggcagcgcct gtccatccac atcaacaaga aagaaaaccc caaccaaacg gccgtcatca
2821agcccttccc caagagcacg gagagccgtg gcctgggcgc tggcgctggc gcaggcggga
2881gcgctggggg cgtgggggcc acgggcggtg cgggctgcgc aggcgccggc ccaggcgggc
2941ccgagtcccc agacgccggc cccaaggcgc tgtatgatgt ggccgaggct gaggagcact
3001tcccggcgcc cgcgcggccg cgctcaccgt cgcccatcag cacgctgagc caccgcgcgg
3061gctcggccag ccgcacggac gacgatgtgc cgtcgctgca ctcggagcct gtggcgcgca
3121gcagctcctc gcagggctcc ctcatggagc agatcagcag tgtggtcacc cgcttcacgg
3181ccaacatcag cgagctcaac tccatgatgc tgtccaccgc ggcccccagc cccggcgtcg
3241gcgccccgct ctgctcgtcc tacctgaTCC CCAAAGAGAT CCAGTTGCCC ACGACCATGA
3301CGACCTTTGC CGAAATCCAG CCTCTGCCGG CCATCGAAGT CACGGGCGGC GCGCAGCCCG
3361CGGCAGGGGC GCAGGCGGCT GGGGACGCGG CCCGGGAGAG CCCCGCGGCC GGTCCCGAGG
3421CTGCGGCCGC CAAGCCAGAC CTGGAGGAGC TGGTGGCTCT CACCCCGCCG TCCCCCTTCA
3481GAGACTCGGT GGACTCGGGG AGCACAACCC CCAACTCGCC AGTGTCCGAG TCGGCCCTCT
3541GTATCCCGTC GTCTCCCAAA TATGACACTC TTATCATAAG AGATTACACT CAGAGCTCCT
3601CGTCGTTGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
3661CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
3721TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCATCT CCACAAAATC GCTAAGCAAC
3781GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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