Construct: ORF TRCN0000489252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020103.1_s317c1
- DNA Barcode:
- GAAATGTCCACACCCCCCCTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRK (54861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489252
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54861 | SNRK | SNF related kinase | NM_001100594.1 | 99.9% | 100% | 16C>A |
| 2 | human | 54861 | SNRK | SNF related kinase | NM_017719.5 | 99.9% | 100% | 16C>A |
| 3 | human | 54861 | SNRK | SNF related kinase | XM_005265245.3 | 99.9% | 100% | 16C>A |
| 4 | human | 54861 | SNRK | SNF related kinase | NM_001330750.1 | 73% | 73% | 0_1ins618 |
| 5 | mouse | 20623 | Snrk | SNF related kinase | NM_001164572.1 | 82.4% | 88.9% | (many diffs) |
| 6 | mouse | 20623 | Snrk | SNF related kinase | NM_133741.2 | 82.4% | 88.9% | (many diffs) |
| 7 | mouse | 20623 | Snrk | SNF related kinase | XM_006511998.3 | 82.4% | 88.9% | (many diffs) |
| 8 | mouse | 20623 | Snrk | SNF related kinase | XM_006511999.3 | 82.4% | 88.9% | (many diffs) |
| 9 | mouse | 20623 | Snrk | SNF related kinase | XM_006512000.3 | 82.4% | 88.9% | (many diffs) |
| 10 | mouse | 20623 | Snrk | SNF related kinase | XM_006512002.3 | 82.4% | 88.9% | (many diffs) |
| 11 | mouse | 20623 | Snrk | SNF related kinase | XM_006512003.3 | 82.4% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 105
- ORF end:
- 2400
- ORF length:
- 2295
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcacagaat tctgttggac cagcatggca ggatttaaga 121 gagggtatga tggaaagatt gctggattat atgatctgga taaaaccttg ggtcgaggcc 181 attttgccgt ggttaaactt gccaggcatg tctttacggg tgaaaaggtg gcagtaaaag 241 ttattgacaa gacaaaactg gacactctag ctactggtca tcttttccag gaagtgagat 301 gcatgaaact agtgcagcat cctaacatcg tccgccttta tgaagttatt gacacccaga 361 ccaaactata tcttattcta gaacttgggg atggaggaga tatgtttgat tatataatga 421 aacatgagga gggtcttaat gaagacttgg ccaagaagta ttttgctcag atagttcatg 481 ctatatctta ttgccataaa ctccatgtgg ttcacagaga cttaaaacca gagaatgtag 541 tcttctttga aaaacaaggt cttgtaaagt tgacagactt tgggttcagc aacaaatttc 601 aaccagggaa gaagctcact acaagctgtg gatctcttgc atattccgct ccagaaattc 661 tgcttggtga tgagtatgat gcacctgcag tagatatttg gagtctggga gtgatccttt 721 tcatgttggt gtgtgggcag ccgccctttc aagaagccaa tgacagtgaa acactgacaa 781 tgatcatgga ttgcaaatat acagtaccat cccatgtgtc taaagagtgt aaagacctaa 841 tcacacggat gctacagaga gatcccaaga gaagggcttc tttagaagag attgaaaatc 901 atccttggct tcagggagtg gacccttcac cagctacaaa gtataacatt ccccttgtgt 961 catacaaaaa tctctcggaa gaggagcaca acagcatcat tcagcgcatg gtgcttgggg 1021 acatagcgga tcgagacgcc attgtagaag ccctggaaac caacaggtat aaccatatca 1081 cagccacata cttcctgctg gctgaaagga tcctgagaga aaagcaagag aaagaaatac 1141 agaccagatc tgcaagcccg agcaatatca aggcccagtt taggcagtca tggccaacca 1201 aaattgatgt accccaggac cttgaggatg acctcacggc cactcctttg tcccacgcga 1261 ctgtccctca gtctcctgct cgggctgctg acagtgtcct caatggccac aggagcaaag 1321 gcctgtgtga ctcagctaag aaagatgacc tccctgagtt ggctggacca gcactctcta 1381 cggtgccacc cgcaagctta aaacccacag ccagtgggcg gaagtgtctg ttcagggtgg 1441 aagaagatga agaggaagat gaggaggaca agaaacccat gtccctctca acacaagtgg 1501 ttttgcgccg gaagccatct gtaaccaacc gcctgacatc caggaagagt gcgcccgtcc 1561 tcaaccagat ctttgaggaa ggggaatctg acgatgagtt tgacatggat gagaatctgc 1621 ctcccaagtt gagcaggtta aagatgaata tagcttctcc aggtacagtt cacaaacgct 1681 accaccggag gaaaagtcag ggccggggct ccagctgcag tagttcggag accagtgatg 1741 atgattctga aagccggcgg cggctcgata aagatagcgg gttcacctac tcctggcacc 1801 gacgggatag cagcgagggg ccccctggca gtgaggggga tggcgggggc cagagcaagc 1861 caagcaatgc cagtggaggg gtggacaagg ccagccccag tgagaacaat gctggtgggg 1921 gcagtccctc cagcggctcg ggtggcaacc ccaccaatac atcgggtacc acacgccgct 1981 gtgccggccc cagcaactcc atgcagctgg cctctcgcag tgctggggag ctcgttgaga 2041 gcctcaaact catgagcctc tgcctcggct cccagcttca tggGAGCACC AAGTACATTA 2101 TTGATCCACA GAATGGCTTG TCATTTTCCA GTGTGAAAGT CCAAGAGAAA TCTACGTGGA 2161 AAATGTGCAT TAGCTCCACA GGGAATGCAG GGCAGGTCCC TGCAGTGGGC GGCATAAAGT 2221 TTTTCTCTGA CCACATGGCA GATACCACCA CTGAATTGGA ACGGATAAAG AGCAAGAACC 2281 TGAAAAATAA CGTGCTGCAG CTACCTCTGT GCGAAAAGAC CATCTCTGTG AACATCCAGC 2341 GGAACCCTAA GGAGGGGCTG CTGTGCGCAT CCAGCCCAGC CAGCTGTTGC CATGTCATCA 2401 AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2461 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2521 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GAAATGTCCA CACCCCCCCT 2581 TTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt