Construct: ORF TRCN0000489266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021705.1_s317c1
- DNA Barcode:
- GTTACTGTCGCAATGCCTAACCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FRMD5 (84978)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_032892.5 | 100% | 100% | |
| 2 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001322950.2 | 89.1% | 84.5% | 1476_1477ins179;1524_1525insTGACACC |
| 3 | human | 84978 | FRMD5 | FERM domain containing 5 | XM_005254730.3 | 88.6% | 84.6% | (many diffs) |
| 4 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001322949.2 | 86.7% | 84.1% | (many diffs) |
| 5 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001322951.2 | 80.7% | 78.1% | (many diffs) |
| 6 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001286490.2 | 72.6% | 68.4% | (many diffs) |
| 7 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001286491.2 | 58.9% | 56.9% | 0_1ins613;25_26ins89 |
| 8 | human | 84978 | FRMD5 | FERM domain containing 5 | XR_931927.3 | 36.4% | 1_201del;1912_4685del | |
| 9 | human | 84978 | FRMD5 | FERM domain containing 5 | NR_104455.2 | 31.8% | (many diffs) | |
| 10 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_006499269.3 | 92.6% | 98.4% | (many diffs) |
| 11 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_011239466.2 | 88.1% | 93.6% | (many diffs) |
| 12 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317742.1 | 82.1% | 83.1% | (many diffs) |
| 13 | mouse | 228564 | Frmd5 | FERM domain containing 5 | NM_172673.3 | 81% | 81.9% | (many diffs) |
| 14 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317740.1 | 80.2% | 82.2% | (many diffs) |
| 15 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317739.1 | 78.1% | 79.2% | (many diffs) |
| 16 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317738.1 | 76.4% | 78.7% | (many diffs) |
| 17 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317734.1 | 76.3% | 78.4% | (many diffs) |
| 18 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317736.1 | 75.9% | 77.9% | (many diffs) |
| 19 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_011239469.2 | 74.1% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1779
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctgagcagg ttgatgagcg gcagcagcag gagcctggag cgcgagtaca 121 gctgcaccgt gcggctgctg gacgacagcg agtacacctg caccatccag agagatgcca 181 aaggccagta cctgtttgac cttctttgcc accatctgaa cctacttgag aaagactatt 241 ttggtatccg ctttgtagac ccagataagc agcggcattg gctggaattt acaaagtctg 301 tggtgaaaca attgagatcc cagcctccat tcaccatgtg cttccgtgtg aagttttatc 361 ctgcagaccc tgctgctctg aaagaagaaa taaccaggta tttagtcttc ctgcagatca 421 aaagggatct ctaccatggc cgactcctct gtaaaacatc ggatgctgcc ttgttagcag 481 cttacatcct tcaagcggag attggggatt atgactcagg gaaacaccct gaaggctaca 541 gctccaagtt ccagtttttc cctaaacatt cagagaagct ggaaaggaaa attgctgaga 601 ttcacaagac ggaactgagt ggtcaaacac cagcaacatc agagctgaac ttcttaagaa 661 aagcacagac attggaaaca tatggagtgg atcctcaccc atgtaaggac gtgtcaggaa 721 atgctgcatt tctggccttc actccttttg ggtttgttgt tcttcaagga aacaagaggg 781 tccacttcat taaatggaat gaggtgacca agctgaaatt tgaaggaaag actttctatt 841 tatacgtaag tcagaaagag gaaaagaaaa ttattcttac atattttgct ccaactcctg 901 aagcgtgtaa gcacctctgg aaatgtggaa tcgagaacca agccttctac aagctggaga 961 agtcaagcca agtccgcaca gtgtccagca gcaatttatt ctttaaaggg agccggttcc 1021 gatacagtgg ccgagttgca aaggaagtca tggaatcaag tgctaagatc aaacgggagc 1081 caccggaaat acacagagca gggatggttc ccagccggag ctgtccctcc ataacccatg 1141 gcccaaggct gagcagcgtc cccaggaccc gcagaagagc tgttcacatc tccatcatgg 1201 aaggcctaga gtccttacgg gacagtgccc attccacacc agtgcgttcc acttcccatg 1261 gggacacctt cctgcctcac gtgagaagca gccggacaga tagcaatgag cgagtagctg 1321 tgattgcaga cgaggcctac agccctgcag acagcgtgct gcccacccct gtggctgagc 1381 acagcctgga gctgatgttg ctttcccggc agatcaatgg agccaccTGC AGCATTGAGG 1441 AGGAGAAGGA ATCTGAAGCC AGCACCCCAA CTGCTACAGA GGTGGAGGCC CTTGGGGGAG 1501 AGCTGAGGGC CCTGTGTCAG GGGCACAGCG GGCCCGAGGA GGAACAGGTG AATAAGTTTG 1561 TTCTAAGTGT CCTCCGTTTG CTCCTTGTGA CCATGGGACT CCTCTTTGTT TTGCTCCTCC 1621 TCCTGATCAT CCTTACCGAG TCTGACCTTG ACATTGCCTT TTTCCGTGAT ATCCGCCAGA 1681 CCCCCGAGTT TGAACAATTC CACTATCAAT ACTTTTGTCC CCTCAGGCGA TGGTTTGCCT 1741 GCAAAATCCG CTCAGTGGTG AGCCTGCTCA TTGACACCTA GAACCCAGCT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGAGTT ACTGTCGCAA TGCCTAACCA TACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t