Construct: ORF TRCN0000489368

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020070.1_s317c1
DNA Barcode:
CCGATGCCTGTCGCCGTCTGGTGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IGF1R (3480)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489368
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3480 IGF1R insulin like growth factor ... NM_000875.5 99.9% 99.9% 4101_4102insG
2 human 3480 IGF1R insulin like growth factor ... NM_001291858.2 99.9% 99.7% 2782_2783insCAG;4098_4099insG
3 human 3480 IGF1R insulin like growth factor ... XM_017022136.1 97.2% 96.4% (many diffs)
4 human 3480 IGF1R insulin like growth factor ... XM_017022137.1 97.2% 96.4% (many diffs)
5 human 3480 IGF1R insulin like growth factor ... XM_017022138.1 97.1% 96.2% (many diffs)
6 human 3480 IGF1R insulin like growth factor ... XM_017022139.1 91.1% 91% 0_1ins363;3738_3739insG
7 human 3480 IGF1R insulin like growth factor ... XM_011521516.2 77.8% 77.7% 0_1ins909;3192_3193insG
8 human 3480 IGF1R insulin like growth factor ... XM_024449913.1 77.8% 77.7% 0_1ins909;3192_3193insG
9 human 3480 IGF1R insulin like growth factor ... XM_011521517.2 67.4% 67.3% 0_1ins1335;2766_2767insG
10 mouse 16001 Igf1r insulin-like growth factor ... NM_010513.2 89.4% 95.9% (many diffs)
11 mouse 16001 Igf1r insulin-like growth factor ... XM_006540643.3 89.3% 95.8% (many diffs)
12 mouse 16001 Igf1r insulin-like growth factor ... XM_006540642.3 87.4% 93.7% (many diffs)
13 mouse 16001 Igf1r insulin-like growth factor ... XM_006540641.3 87.3% 93.7% (many diffs)
14 mouse 16001 Igf1r insulin-like growth factor ... XM_006540645.3 75.2% 80% (many diffs)
15 mouse 16001 Igf1r insulin-like growth factor ... XM_006540644.3 73.5% 78.2% (many diffs)
16 mouse 16001 Igf1r insulin-like growth factor ... XM_017321986.1 67.6% 73.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4173
ORF length:
4104
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gaagtctggc tccggaggag ggtccccgac ctcgctgtgg gggctcctgt
121ttctctccgc cgcgctctcg ctctggccga cgagtggaga aatctgcggg ccaggcatcg
181acatccgcaa cgactatcag cagctgaagc gcctggagaa ctgcacggtg atcgagggct
241acctccacat cctgctcatc tccaaggccg aggactaccg cagctaccgc ttccccaagc
301tcacggtcat taccgagtac ttgctgctgt tccgagtggc tggcctcgag agcctcggag
361acctcttccc caacctcacg gtcatccgcg gctggaaact cttctacaac tacgccctgg
421tcatcttcga gatgaccaat ctcaaggata ttgggcttta caacctgagg aacattactc
481ggggggccat caggattgag aaaaatgctg acctctgtta cctctccact gtggactggt
541ccctgatcct ggatgcggtg tccaataact acattgtggg gaataagccc ccaaaggaat
601gtggggacct gtgtccaggg accatggagg agaagccgat gtgtgagaag accaccatca
661acaatgagta caactaccgc tgctggacca caaaccgctg ccagaaaatg tgcccaagca
721cgtgtgggaa gcgggcgtgc accgagaaca atgagtgctg ccaccccgag tgcctgggca
781gctgcagcgc gcctgacaac gacacggcct gtgtagcttg ccgccactac tactatgccg
841gtgtctgtgt gcctgcctgc ccgcccaaca cctacaggtt tgagggctgg cgctgtgtgg
901accgtgactt ctgcgccaac atcctcagcg ccgagagcag cgactccgag gggtttgtga
961tccacgacgg cgagtgcatg caggagtgcc cctcgggctt catccgcaac ggcagccaga
1021gcatgtactg catcccttgt gaaggtcctt gcccgaaggt ctgtgaggaa gaaaagaaaa
1081caaagaccat tgattctgtt acttctgctc agatgctcca aggatgcacc atcttcaagg
1141gcaatttgct cattaacatc cgacggggga ataacattgc ttcagagctg gagaacttca
1201tggggctcat cgaggtggtg acgggctacg tgaagatccg ccattctcat gccttggtct
1261ccttgtcctt cctaaaaaac cttcgcctca tcctaggaga ggagcagcta gaagggaatt
1321actccttcta cgtcctcgac aaccagaact tgcagcaact gtgggactgg gaccaccgca
1381acctgaccat caaagcaggg aaaatgtact ttgctttcaa tcccaaatta tgtgtttccg
1441aaatttaccg catggaggaa gtgacgggga ctaaagggcg ccaaagcaaa ggggacataa
1501acaccaggaa caacggggag agagcctcct gtgaaagtga cgtcctgcat ttcacctcca
1561ccaccacgtc gaagaatcgc atcatcataa cctggcaccg gtaccggccc cctgactaca
1621gggatctcat cagcttcacc gtttactaca aggaagcacc ctttaagaat gtcacagagt
1681atgatgggca ggatgcctgc ggctccaaca gctggaacat ggtggacgtg gacctcccgc
1741ccaacaagga cgtggagccc ggcatcttac tacatgggct gaagccctgg actcagtacg
1801ccgtttacgt caaggctgtg accctcacca tggtggagaa cgaccatatc cgtggggcca
1861agagtgagat cttgtacatt cgcaccaatg cttcagttcc ttccattccc ttggacgttc
1921tttcagcatc gaactcctct tctcagttaa tcgtgaagtg gaaccctccc tctctgccca
1981acggcaacct gagttactac attgtgcgct ggcagcggca gcctcaggac ggctaccttt
2041accggcacaa ttactgctcc aaagacaaaa tccccatcag gaagtatgcc gacggcacca
2101tcgacattga ggaggtcaca gagaacccca agactgaggt gtgtggtggg gagaaagggc
2161cttgctgcgc ctgccccaaa actgaagccg agaagcaggc cgagaaggag gaggctgaat
2221accgcaaagt ctttgagaat ttcctgcaca actccatctt cgtgcccaga cctgaaagga
2281agcggagaga tgtcatgcaa gtggccaaca ccaccatgtc cagccgaagc aggaacacca
2341cggccgcaga cacctacaac atcaccgacc cggaagagct ggagacagag taccctttct
2401ttgagagcag agtggataac aaggagagaa ctgtcatttc taaccttcgg cctttcacat
2461tgtaccgcat cgatatccac agctgcaacc acgaggctga gaagctgggc tgcagcgcct
2521ccaacttcgt ctttgcaagg actatgcccg cagaaggagc agatgacatt cctgggccag
2581tgacctggga gccaaggcct gaaaactcca tctttttaaa gtggccggaa cctgagaatc
2641ccaatggatt gattctaatg tatgaaataa aatacggatc acaagttgag gatcagcgag
2701aatgtgtgtc cagacaggaa tacaggaagt atggaggggc caagctaaac cggctaaacc
2761cggggaacta cacagcccgg attcaggcca catctctctc tgggaatggg tcgtggacag
2821atcctgtgtt cttctatgtc caggccaaaa caggatatga aaacttcatc catctgatca
2881tcgctctgcc cgtcgctgtc ctgttgatcg tgggagggtt ggtgattatg ctgtacgtct
2941tccatagaaa gagaaataac agcaggctgg ggaatggagt gctgtatgcc tctgtgaacc
3001cggagtactt cagcgctgct gatgtgtacg ttcctgatga gtgggaggtg gctcgggaga
3061agatcaccat gagccgggaa cttgggcagg ggtcgtttgg gatggtctat gaaggagttg
3121ccaagggtgt ggtgaaagat gaacctgaaa ccagagtggc cattaaaaca gtgaacgagg
3181ccgcaagcat gcgtgagagg attgagtttc tcaacgaagc ttctgtgatg aaggagttca
3241attgtcacca tgtggtgcga ttgctgggtg tggtgtccca aggccagcca acactggtca
3301tcatggaact gatgacacgg ggcgatctca aaagttatct ccggtctctg aggccagaaa
3361tggagaataa tccagtccta gcacctccaa gcctgagcaa gatgattcag atggccggag
3421agattgcaga cggcatggca tacctcaacg ccaataagtt cgtccacaga gaccttgctg
3481cccggaattg catggtagcc gaagatttca cagtcaaaat cggagatttt ggtatgacgc
3541gagatatcta tgagacagac tattaccgga aaggagggaa agggctgctg cccgtgcgct
3601ggatgtctcc tgagtccctc aaggatggag tcttcaccac ttactcggac gtctggtcct
3661tcggggtcgt cctctgggag atcgccacac tggccgagca gccctaccag ggcttgtcca
3721acgagcaagt ccttcgcttc gtcatggagg gcggccttct ggacaagcca gacaactgtc
3781ctgacatgct gtttgaactg atgcgcatgt gctggcagta taaccccaag atgaggccTT
3841CCTTCCTGGA GATCATCAGC AGCATCAAAG AGGAGATGGA GCCTGGCTTC CGGGAGGTCT
3901CCTTCTACTA CAGCGAGGAG AACAAGCTGC CCGAGCCGGA GGAGCTGGAC CTGGAGCCAG
3961AGAACATGGA GAGCGTCCCC CTGGACCCCT CGGCCTCCTC GTCCTCCCTG CCACTGCCCG
4021ACAGACACTC AGGACACAAG GCCGAGAACG GCCCCGGCCC TGGGGTGCTG GTCCTCCGCG
4081CCAGCTTCGA CGAGAGACAG CCTTACGCCC ACATGAACGG GGGCCGCAAG AACGAGCGGG
4141CCTTGCCGCT GCCCCAGTCT TCGACCTGCG ACCCAGCTTT CTTGTACAAA GTGGTTGATA
4201TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
4261CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGAT
4321GCCTGTCGCC GTCTGGTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
4381tgaaagatt

Download FASTA (ORF) (Full)