Construct: ORF TRCN0000489477
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019713.1_s317c1
- DNA Barcode:
- CTTACCCCATGCGATACCGACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME1 (4830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489477
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 99.7% | 99.3% | 456_457insG |
| 2 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 85.7% | 85.3% | 1_75del;531_532insG |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 81.2% | 87.5% | (many diffs) |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 81.2% | 87.5% | (many diffs) |
| 5 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 81.2% | 87.5% | (many diffs) |
| 6 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 81.2% | 87.5% | (many diffs) |
| 7 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 53.7% | 50% | (many diffs) |
| 8 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 33.8% | (many diffs) | |
| 9 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | NM_008704.2 | 87.5% | 93.4% | (many diffs) |
| 10 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | XM_030245646.1 | 80.1% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 534
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc aactgtgagc gtaccttcat tgcgatcaaa ccagatgggg 121 tccagcgggg tcttgtggga gagattatca agcgttttga gcagaaagga ttccgccttg 181 ttggtctgaa attcatgcaa gcttccgaag atcttctcaa ggaacactac gttgacctga 241 aggaccgtcc attctttgcc ggcctgGTGA AATACATGCA CTCAGGGCCG GTAGTTGCCA 301 TGGTCTGGGA GGGGCTGAAT GTGGTGAAGA CGGGCCGAGT CATGCTCGGG GAGACCAACC 361 CTGCAGACTC CAAGCCTGGG ACCATCCGTG GAGACTTCTG CATACAAGTT GGCAGGAACA 421 TTATACATGG CAGTGATTCT GTGGAGAGTG CAGAGAAGGA GATCGGCTTG TGGTTTCACC 481 CTGAGGAACT GGTAGATTAC ACGAGCTGTG CTCAGAACTG GATCTATGAA GACCCAGCTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGACTTA CCCCATGCGA TACCGACTGC ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt