Transcript: Mouse NM_008704.2

Mus musculus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), mRNA.

Source:
NCBI, updated 2019-07-16
Taxon:
Mus musculus (mouse)
Gene:
Nme1 (18102)
Length:
1235
CDS:
306..764

Additional Resources:

NCBI RefSeq record:
NM_008704.2
NBCI Gene record:
Nme1 (18102)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008704.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360451 ACGTGCCACTGTTAGATTAAA pLKO_005 894 3UTR 100% 15.000 21.000 N Nme1 n/a
2 TRCN0000360450 CTTTACTGGCCTGGTGAAATA pLKO_005 485 CDS 100% 13.200 18.480 N Nme1 n/a
3 TRCN0000360520 GTGCGCAGAACTGGATCTATG pLKO_005 739 CDS 100% 10.800 15.120 N Nme1 n/a
4 TRCN0000024731 CGATTCTGTAAAGAGCGCAGA pLKO.1 665 CDS 100% 2.160 3.024 N Nme1 n/a
5 TRCN0000360449 GGGACCAGCAACCCTAGATAT pLKO_005 813 3UTR 100% 13.200 9.240 N Nme1 n/a
6 TRCN0000360522 TCAGGACCAGTGGTTGCTATG pLKO_005 513 CDS 100% 6.000 4.200 N Nme1 n/a
7 TRCN0000024729 CAACCCTAGATATTTCTGGAA pLKO.1 821 3UTR 100% 2.640 1.848 N Nme1 n/a
8 TRCN0000024732 GCCTGGTGAAATACATGCACT pLKO.1 493 CDS 100% 2.640 1.848 N Nme1 n/a
9 TRCN0000024733 GTCTGAAGTTTCTGCAGGCTT pLKO.1 415 CDS 100% 2.640 1.848 N Nme1 n/a
10 TRCN0000054518 GTTGGCAGGAACATCATTCAT pLKO.1 639 CDS 100% 5.625 2.813 Y Nme2 n/a
11 TRCN0000287722 GTTGGCAGGAACATCATTCAT pLKO_005 639 CDS 100% 5.625 2.813 Y Nme2 n/a
12 TRCN0000010061 TCCGCCTTGTTGGTCTGAAAT pLKO.1 403 CDS 100% 13.200 9.240 N NME1 n/a
13 TRCN0000234326 TCCGCCTTGTTGGTCTGAAAT pLKO_005 403 CDS 100% 13.200 9.240 N NME1-NME2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008704.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01101 pDONR223 100% 87.7% 94% None (many diffs) n/a
2 ccsbBroad304_01101 pLX_304 0% 87.7% 94% V5 (many diffs) n/a
3 TRCN0000468949 CACCGCTGGGTGCTAGCGAGCCCG pLX_317 73.3% 87.7% 94% V5 (many diffs) n/a
4 ccsbBroadEn_14714 pDONR223 0% 87.7% 94% None (many diffs) n/a
5 ccsbBroad304_14714 pLX_304 0% 87.7% 94% V5 (many diffs) n/a
6 TRCN0000479668 TTTTAGCACTCTCCAGTATATATA pLX_317 69.3% 87.7% 94% V5 (many diffs) n/a
7 TRCN0000488282 CTTCCTTTCGAGACTCGATGACCG pLX_317 63.2% 87.7% 94% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000489477 CTTACCCCATGCGATACCGACTGC pLX_317 56.7% 87.5% 93.4% V5 (many diffs) n/a
9 TRCN0000491954 CGATCTATCACAATTATAACCTTA pLX_317 76.9% 87.5% 94% V5 (not translated due to prior stop codon) (many diffs) n/a
10 ccsbBroadEn_14715 pDONR223 100% 82.7% 90.1% None (many diffs) n/a
11 ccsbBroad304_14715 pLX_304 0% 82.7% 90.1% V5 (many diffs) n/a
12 TRCN0000469993 CCAAGGCATCGCGTCTCAAAGTAA pLX_317 72.2% 82.7% 90.1% V5 (many diffs) n/a
13 TRCN0000492213 TGTACTCCCGGGACTCAGACCACG pLX_317 92.3% 82.7% 90.1% V5 (not translated due to prior stop codon) (many diffs) n/a
14 ccsbBroadEn_10663 pDONR223 100% 43.1% 46.9% None (many diffs) n/a
15 ccsbBroad304_10663 pLX_304 0% 43.1% 46.9% V5 (many diffs) n/a
16 TRCN0000476918 CCTGTCGTTTGGTGACAACTCTGT pLX_317 33.8% 43.1% 46.9% V5 (many diffs) n/a
Download CSV