Transcript: Human NM_001018136.3

Homo sapiens NME1-NME2 readthrough (NME1-NME2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
NME1-NME2 (654364)
Length:
1052
CDS:
109..912

Additional Resources:

NCBI RefSeq record:
NM_001018136.3
NBCI Gene record:
NME1-NME2 (654364)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001018136.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010062 CCGCCTTGTTGGTCTGAAATT pLKO.1 207 CDS 100% 13.200 6.600 Y NME1 n/a
2 TRCN0000196307 GAAATCAGCCTATGGTTTAAG pLKO.1 838 CDS 100% 13.200 6.600 Y NME2 n/a
3 TRCN0000234328 GAAATCAGCCTATGGTTTAAG pLKO_005 838 CDS 100% 13.200 6.600 Y NME1-NME2 n/a
4 TRCN0000318404 GAAATCAGCCTATGGTTTAAG pLKO_005 838 CDS 100% 13.200 6.600 Y NME2 n/a
5 TRCN0000218662 TCATGGCAGTGATTCAGTAAA pLKO_005 804 CDS 100% 13.200 6.600 Y NME1-NME2 n/a
6 TRCN0000010061 TCCGCCTTGTTGGTCTGAAAT pLKO.1 206 CDS 100% 13.200 6.600 Y NME1 n/a
7 TRCN0000234326 TCCGCCTTGTTGGTCTGAAAT pLKO_005 206 CDS 100% 13.200 6.600 Y NME1-NME2 n/a
8 TRCN0000381107 ACCAATCCAGCAGATTCAAAG pLKO_005 733 CDS 100% 10.800 5.400 Y NME2 n/a
9 TRCN0000010108 AGCACTACATTGACCTGAAAG pLKO.1 602 CDS 100% 10.800 5.400 Y NME2 n/a
10 TRCN0000234327 AGCACTACATTGACCTGAAAG pLKO_005 602 CDS 100% 10.800 5.400 Y NME1-NME2 n/a
11 TRCN0000349498 AGCACTACATTGACCTGAAAG pLKO_005 602 CDS 100% 10.800 5.400 Y NME2 n/a
12 TRCN0000381045 GGAGACCAATCCAGCAGATTC pLKO_005 729 CDS 100% 10.800 5.400 Y NME1-NME2 n/a
13 TRCN0000381376 TGAAGGACCGTCCATTCTTTG pLKO_005 272 CDS 100% 10.800 5.400 Y NME1-NME2 n/a
14 TRCN0000197030 GCTCATGACTGGGTCTATGAA pLKO.1 889 CDS 100% 5.625 2.813 Y NME2 n/a
15 TRCN0000318379 GCTCATGACTGGGTCTATGAA pLKO_005 889 CDS 100% 5.625 2.813 Y NME2 n/a
16 TRCN0000054518 GTTGGCAGGAACATCATTCAT pLKO.1 787 CDS 100% 5.625 2.813 Y Nme2 n/a
17 TRCN0000287722 GTTGGCAGGAACATCATTCAT pLKO_005 787 CDS 100% 5.625 2.813 Y Nme2 n/a
18 TRCN0000024906 ACATCATTCATGGCAGTGATT pLKO.1 797 CDS 100% 4.950 2.475 Y Nme2 n/a
19 TRCN0000379743 ATTCATGGCAGTGATTCAGTA pLKO_005 802 CDS 100% 4.950 2.475 Y NME2 n/a
20 TRCN0000010109 CCTATGGTTTAAGCCTGAAGA pLKO.1 846 CDS 100% 4.950 2.475 Y NME2 n/a
21 TRCN0000195380 CTGAAGAACTGGTTGACTACA pLKO.1 860 CDS 100% 4.950 2.475 Y NME2 n/a
22 TRCN0000010055 GCGTACCTTCATTGCGATCAA pLKO.1 123 CDS 100% 4.950 2.475 Y NME1 n/a
23 TRCN0000234329 ACACAACAGCAGTCTCCTTCA pLKO_005 920 3UTR 100% 4.050 2.025 Y NME1-NME2 n/a
24 TRCN0000195555 CTCAAGGAACACTACGTTGAC pLKO.1 250 CDS 100% 4.050 2.025 Y NME1 n/a
25 TRCN0000010063 CGGCCTGGTGAAATACATGCA pLKO.1 294 CDS 100% 2.640 1.320 Y NME1 n/a
26 TRCN0000024732 GCCTGGTGAAATACATGCACT pLKO.1 296 CDS 100% 2.640 1.320 Y Nme1 n/a
27 TRCN0000010110 TTTAAGCCTGAAGAACTGGTT pLKO.1 853 CDS 100% 2.640 1.320 Y NME2 n/a
28 TRCN0000199489 GACTTCTGCATTCAGGTTGGC pLKO.1 772 CDS 100% 2.160 1.080 Y NME2 n/a
29 TRCN0000318433 GACTTCTGCATTCAGGTTGGC pLKO_005 772 CDS 100% 2.160 1.080 Y NME2 n/a
30 TRCN0000010102 TTCATCGCCATCAAGCCGGAC pLKO.1 475 CDS 100% 0.400 0.200 Y NME2 n/a
31 TRCN0000199010 CAGAAGGGATTCCGCCTCGTG pLKO.1 541 CDS 100% 0.000 0.000 Y NME2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001018136.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000470841 CGTGCGTGAACAATGGCCCGGCTC pLX_317 40.9% 99.7% 8.2% V5 (not translated due to prior stop codon) 36A>N;62_63insG n/a
2 ccsbBroadEn_15341 pDONR223 100% 99.5% 8.2% None (many diffs) n/a
3 ccsbBroad304_15341 pLX_304 0% 99.5% 8.2% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_10663 pDONR223 100% 91.4% 91.4% None 0_1ins75 n/a
5 ccsbBroad304_10663 pLX_304 0% 91.4% 91.4% V5 0_1ins75 n/a
6 TRCN0000476918 CCTGTCGTTTGGTGACAACTCTGT pLX_317 33.8% 91.4% 91.4% V5 0_1ins75 n/a
7 ccsbBroadEn_14715 pDONR223 100% 56.9% 56.9% None 1_345del n/a
8 ccsbBroad304_14715 pLX_304 0% 56.9% 56.9% V5 1_345del n/a
9 TRCN0000469993 CCAAGGCATCGCGTCTCAAAGTAA pLX_317 72.2% 56.9% 56.9% V5 1_345del n/a
10 TRCN0000492213 TGTACTCCCGGGACTCAGACCACG pLX_317 92.3% 56.9% 56.9% V5 (not translated due to prior stop codon) 1_345del n/a
11 ccsbBroadEn_01101 pDONR223 100% 53.8% 50.1% None (many diffs) n/a
12 ccsbBroad304_01101 pLX_304 0% 53.8% 50.1% V5 (many diffs) n/a
13 TRCN0000468949 CACCGCTGGGTGCTAGCGAGCCCG pLX_317 73.3% 53.8% 50.1% V5 (many diffs) n/a
14 ccsbBroadEn_14714 pDONR223 0% 53.8% 50.1% None (many diffs) n/a
15 ccsbBroad304_14714 pLX_304 0% 53.8% 50.1% V5 (many diffs) n/a
16 TRCN0000479668 TTTTAGCACTCTCCAGTATATATA pLX_317 69.3% 53.8% 50.1% V5 (many diffs) n/a
17 TRCN0000488282 CTTCCTTTCGAGACTCGATGACCG pLX_317 63.2% 53.8% 50.1% V5 (not translated due to prior stop codon) (many diffs) n/a
18 TRCN0000489477 CTTACCCCATGCGATACCGACTGC pLX_317 56.7% 53.7% 50% V5 (many diffs) n/a
19 TRCN0000491954 CGATCTATCACAATTATAACCTTA pLX_317 76.9% 53.7% 50.1% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.