Construct: ORF TRCN0000489480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019496.1_s317c1
- DNA Barcode:
- GAAATCAATTATGGTCTTATGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMK1 (3984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_002314.4 | 99.9% | 100% | 1353C>T |
| 2 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_001204426.2 | 93.8% | 92.8% | (many diffs) |
| 3 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_010717.3 | 87.9% | 95.2% | (many diffs) |
| 4 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_001305875.1 | 82.6% | 84.8% | (many diffs) |
| 5 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | XM_006504396.3 | 72.6% | 68.5% | (many diffs) |
| 6 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NR_131247.1 | 43.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 2034
- ORF length:
- 1941
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccaga tctgtcgagt gcatgaggtt gacgctactt tgttgcacct 121 ggagggaaga acgtatggga gaggaaggaa gcgagttgcc cgtgtgtgca agctgcggcc 181 agaggatcta tgatggccag tacctccagg ccctgaacgc ggactggcac gcagactgct 241 tcaggtgttg tgactgcagt gcctccctgt cgcaccagta ctatgagaag gatgggcagc 301 tcttctgcaa gaaggactac tgggcccgct atggcgagtc ctgccatggg tgctctgagc 361 aaatcaccaa gggactggtt atggtggctg gggagctgaa gtaccacccc gagtgtttca 421 tctgcctcac gtgtgggacc tttatcggtg acggggacac ctacacgctg gtggagcact 481 ccaagctgta ctgcgggcac tgctactacc agactgtggt gacccccgtc atcgagcaga 541 tcctgcctga ctcccctggc tcccacctgc cccacaccgt caccctggtg tccatcccag 601 cctcatctca tggcaagcgt ggactttcag tctccattga ccccccgcac ggcccaccgg 661 gctgtggcac cgagcactca cacaccgtcc gcgtccaggg agtggatccg ggctgcatga 721 gcccagatgt gaagaattcc atccacgtcg gagaccggat cttggaaatc aatggcacgc 781 ccatccgaaa tgtgcccctg gacgagattg acctgctgat tcaggaaacc agccgcctgc 841 tccagctgac cctcgagcat gaccctcacg atacactggg ccacgggctg gggcctgaga 901 ccagccccct gagctctccg gcttatactc ccagcgggga ggcgggcagc tctgcccggc 961 agaaacctgt cttgaggagc tgcagcatcg acaggtctcc gggcgctggc tcactgggct 1021 ccccggcctc ccagcgcaag gacctgggtc gctctgagtc cctccgcgta gtctgccggc 1081 cacaccgcat cttccggccg tcggacctca tccacgggga ggtgctgggc aagggctgct 1141 tcggccaggc tatcaaggtg acacaccgtg agacaggtga ggtgatggtg atgaaggagc 1201 tgatccggtt cgacgaggag acccagagga cgttcctcaa ggaggtgaag gtcatgcgat 1261 gcctggaaca ccccaacgtg ctcaagttca tcggggtgct ctacaaggac aagaggctca 1321 acttcatcac tgagtacatc aagggcggca cgctccgggg catcatcaag agcatggaca 1381 gccagtaccc atggagccag agagtgagct ttgccaagga catcgcatca gggatggcct 1441 accttcactc catgaacatc atccaccgag acctcaactc ccacaactgc ctggtccgcg 1501 agaacaagaa tgtggtggtg gctgacttcg ggctggcgcg tctcatggtg gacgagaaga 1561 ctcagcctga gggcctgcgg agcctcaaga agccagaccg caagaagcgc tacaccgtgg 1621 tgggcaaccc ctactggatg gcacctgaga tgatcaacgg ccgcagctat gatgagaagg 1681 tggatgtgtt ctccTTTGGG ATCGTCCTGT GCGAGATCAT CGGGCGGGTG AACGCAGACC 1741 CTGACTACCT GCCCCGCACC ATGGACTTTG GCCTCAACGT GCGAGGATTC CTGGACCGCT 1801 ACTGCCCCCC AAACTGCCCC CCGAGCTTCT TCCCCATCAC CGTGCGCTGT TGCGATCTGG 1861 ACCCCGAGAA GAGGCCATCC TTTGTGAAGC TGGAACACTG GCTGGAGACC CTCCGCATGC 1921 ACCTGGCCGG CCACCTGCCA CTGGGCCCAC AGCTGGAGCA GCTGGACAGA GGTTTCTGGG 1981 AGACCTACCG GCGCGGCGAG AGCGGACTGC CTGCCCACCC TGAGGTCCCC GACAAGGGTG 2041 GGCGCGCCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2101 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2161 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGAAATC AATTATGGTC TTATGGATAC 2221 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt