Construct: ORF TRCN0000489511

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021944.1_s317c1
DNA Barcode:
CTCATTCCAAATATTCTTATAAAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EGFR (1956)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489511
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2239_2248delinsC 99.9% 91.6% 2352G>A;3285_3286insA;3300C>A
2 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2240_2254del 99.7% 91.5% (many diffs)
3 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2248G>C 99.6% 91.4% (many diffs)
4 human 1956 EGFR epidermal growth factor rec... NM_005228.5 99.6% 91.3% (many diffs)
5 human 1956 EGFR epidermal growth factor rec... NM_001346899.1 95.8% 87.6% (many diffs)
6 human 1956 EGFR epidermal growth factor rec... NM_001346900.2 95.2% 87% (many diffs)
7 human 1956 EGFR epidermal growth factor rec... NM_001346898.2 92.3% 94.1% (many diffs)
8 human 1956 EGFR epidermal growth factor rec... NM_001346897.2 88.6% 90.2% (many diffs)
9 human 1956 EGFR epidermal growth factor rec... NM_001346941.2 77% 66.8% (many diffs)
10 human 1956 EGFR epidermal growth factor rec... NM_201284.2 56.2% 52.7% (many diffs)
11 human 1956 EGFR epidermal growth factor rec... NM_201282.2 51.9% 53.9% 1881G>A;1883C>G;1884_1885ins1738
12 human 1956 EGFR epidermal growth factor rec... NM_201283.1 33.4% 34.6% 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2406
13 mouse 13649 Egfr epidermal growth factor rec... NM_207655.2 84.4% 82.8% (many diffs)
14 mouse 13649 Egfr epidermal growth factor rec... NM_007912.4 45% 48.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3558
ORF length:
3486
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg ctgctggctg
121cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc acgagtaaca
181agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg atgttcaata
241actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat tatgatcttt
301ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc aacacagtgg
361agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac gaaaattcct
421atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag gagctgccca
481tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt
541gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc agcaacatgt
601cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg
661ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc atctgtgccc
721agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac aaccagtgtg
781ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa ttccgagacg
841aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc acgtaccaga
901tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc
961gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg
1021agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt
1081gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta
1141aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta
1201ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa
1261ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc
1321tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt
1381ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa
1441gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact
1501ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa
1561acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg
1621gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg
1681acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac
1741agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag
1801acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg
1861caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt
1921gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc
1981caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc
2041tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc
2101gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag
2161aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag
2221tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga
2281aagttaaaat tcccgtcgct atcaaggaac caacatctcc gaaagccaac aaggaaatcc
2341tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca cgtgtgccgc ctgctgggca
2401tctgcctcac ctccaccgtg caactcatca cgcagctcat gcccttcggc tgcctcctgg
2461actatgtccg ggaacacaaa gacaatattg gctcccagta cctgctcaac tggtgtgtgc
2521agatcgcaaa gggcatgaac tacttggagg accgtcgctt ggtgcaccgc gacctggcag
2581ccaggaacgt actggtgaaa acaccgcagc atgtcaagat cacagatttt gggctggcca
2641aactgctggg tgcggaagag aaagaatacc atgcagaagg aggcaaagtg cctatcaagt
2701ggatggcatt ggaatcaatt ttacacagaa tctataccca ccagagtgat gtctggagct
2761acggggtgac cgtttgggag ttgatgacct ttggatccaa gccatatgac ggaatccctg
2821ccagcgagat ctcctccatc ctggagaaag gagaacgcct ccctcagcca cccatatgta
2881ccatcgatgt ctacatgatc atggtcaagt gctggatgat agacgcagat agtcgcccaa
2941agttccgtga gttgatcatc gaattctcca aaatggcccg agacccccag cgctaccttg
3001tcattcaggg ggatgaaaga atgcatttgc caagtcctac agactccaac ttctaccgtg
3061ccctgatgga tgaagaagac atggacgacg tggtggatgc cgacgagtac ctcatcccac
3121agcagggctt cttcagcagc ccctccacgt cacggactcc cctcctgagc tctctgagtg
3181caaccagcaa caattccacc gtggcttgca ttgatagaaa tgggctgcaa agctgtccca
3241tcaaggaaga cagcttcttg cagcgataca gctcagaccc cacaggcgcc ttgactgagg
3301acagcataga cgacaccTTC CTCCCAGTGC CTGAATACAT AAACCAGTCC GTTCCCAAAA
3361AGGCCCGCTG GATCTGTGCA GAATCCTGTC TATCACAATC AGCCTCTGAA CCCCGCGCCC
3421AGCAGAGACC CACACTACCA GGACCCCCAC AGCACTGCAG TGGGCAACCC CGAGTATCTC
3481AACACTGTCC AGCCCACCTG TGTCAACAGC ACATTCGACA GCCCTGCCCA CTGGGCCCAG
3541AAAGGCAGCC ACCAAATTAG CCTGGACAAC CCTGACTACC AGCAGGACTT CTTTCCCAAG
3601GAAGCCAAGC CAAATGGCAT CTTTAAGGGC TCCACAGCTG AAAATGCAGA ATACCTAAGG
3661GTCGCGCCAC AAAGCAGTGA ATTTATTGGA GCATGAGACC CAGCTTTCTT GTACAAAGTG
3721GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA
3781ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC
3841GACTCATTCC AAATATTCTT ATAAAGACGC GTTAAGTCga caatcaacct ctggattaca
3901aaatttgtga aagatt

Download FASTA (ORF) (Full)

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