Construct: ORF TRCN0000489511
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021944.1_s317c1
- DNA Barcode:
- CTCATTCCAAATATTCTTATAAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489511
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2239_2248delinsC | 99.9% | 91.6% | 2352G>A;3285_3286insA;3300C>A |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 99.7% | 91.5% | (many diffs) |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2248G>C | 99.6% | 91.4% | (many diffs) |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 99.6% | 91.3% | (many diffs) |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 95.8% | 87.6% | (many diffs) |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 95.2% | 87% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 92.3% | 94.1% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 88.6% | 90.2% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 77% | 66.8% | (many diffs) |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.2% | 52.7% | (many diffs) |
| 11 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 51.9% | 53.9% | 1881G>A;1883C>G;1884_1885ins1738 |
| 12 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 33.4% | 34.6% | 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2406 |
| 13 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 84.4% | 82.8% | (many diffs) |
| 14 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 45% | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3558
- ORF length:
- 3486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg ctgctggctg 121 cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc acgagtaaca 181 agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg atgttcaata 241 actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat tatgatcttt 301 ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc aacacagtgg 361 agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac gaaaattcct 421 atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag gagctgccca 481 tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt 541 gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc agcaacatgt 601 cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg 661 ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc atctgtgccc 721 agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac aaccagtgtg 781 ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa ttccgagacg 841 aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc acgtaccaga 901 tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc 961 gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg 1021 agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt 1081 gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta 1141 aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta 1201 ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa 1261 ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc 1321 tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt 1381 ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa 1441 gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact 1501 ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa 1561 acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg 1621 gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg 1681 acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac 1741 agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag 1801 acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg 1861 caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt 1921 gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc 1981 caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc 2041 tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc 2101 gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag 2161 aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag 2221 tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga 2281 aagttaaaat tcccgtcgct atcaaggaac caacatctcc gaaagccaac aaggaaatcc 2341 tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca cgtgtgccgc ctgctgggca 2401 tctgcctcac ctccaccgtg caactcatca cgcagctcat gcccttcggc tgcctcctgg 2461 actatgtccg ggaacacaaa gacaatattg gctcccagta cctgctcaac tggtgtgtgc 2521 agatcgcaaa gggcatgaac tacttggagg accgtcgctt ggtgcaccgc gacctggcag 2581 ccaggaacgt actggtgaaa acaccgcagc atgtcaagat cacagatttt gggctggcca 2641 aactgctggg tgcggaagag aaagaatacc atgcagaagg aggcaaagtg cctatcaagt 2701 ggatggcatt ggaatcaatt ttacacagaa tctataccca ccagagtgat gtctggagct 2761 acggggtgac cgtttgggag ttgatgacct ttggatccaa gccatatgac ggaatccctg 2821 ccagcgagat ctcctccatc ctggagaaag gagaacgcct ccctcagcca cccatatgta 2881 ccatcgatgt ctacatgatc atggtcaagt gctggatgat agacgcagat agtcgcccaa 2941 agttccgtga gttgatcatc gaattctcca aaatggcccg agacccccag cgctaccttg 3001 tcattcaggg ggatgaaaga atgcatttgc caagtcctac agactccaac ttctaccgtg 3061 ccctgatgga tgaagaagac atggacgacg tggtggatgc cgacgagtac ctcatcccac 3121 agcagggctt cttcagcagc ccctccacgt cacggactcc cctcctgagc tctctgagtg 3181 caaccagcaa caattccacc gtggcttgca ttgatagaaa tgggctgcaa agctgtccca 3241 tcaaggaaga cagcttcttg cagcgataca gctcagaccc cacaggcgcc ttgactgagg 3301 acagcataga cgacaccTTC CTCCCAGTGC CTGAATACAT AAACCAGTCC GTTCCCAAAA 3361 AGGCCCGCTG GATCTGTGCA GAATCCTGTC TATCACAATC AGCCTCTGAA CCCCGCGCCC 3421 AGCAGAGACC CACACTACCA GGACCCCCAC AGCACTGCAG TGGGCAACCC CGAGTATCTC 3481 AACACTGTCC AGCCCACCTG TGTCAACAGC ACATTCGACA GCCCTGCCCA CTGGGCCCAG 3541 AAAGGCAGCC ACCAAATTAG CCTGGACAAC CCTGACTACC AGCAGGACTT CTTTCCCAAG 3601 GAAGCCAAGC CAAATGGCAT CTTTAAGGGC TCCACAGCTG AAAATGCAGA ATACCTAAGG 3661 GTCGCGCCAC AAAGCAGTGA ATTTATTGGA GCATGAGACC CAGCTTTCTT GTACAAAGTG 3721 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 3781 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 3841 GACTCATTCC AAATATTCTT ATAAAGACGC GTTAAGTCga caatcaacct ctggattaca 3901 aaatttgtga aagatt