Construct: ORF TRCN0000489537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019445.1_s317c1
- DNA Barcode:
- CCCGTCCTGAATGCGCACCTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SULF1 (23213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23213 | SULF1 | sulfatase 1 | NM_001128204.2 | 99.9% | 99.8% | 2613_2614insG |
| 2 | human | 23213 | SULF1 | sulfatase 1 | NM_001128205.2 | 99.9% | 99.8% | 2613_2614insG |
| 3 | human | 23213 | SULF1 | sulfatase 1 | NM_001128206.2 | 99.9% | 99.8% | 2613_2614insG |
| 4 | human | 23213 | SULF1 | sulfatase 1 | NM_015170.2 | 99.9% | 99.8% | 2613_2614insG |
| 5 | human | 23213 | SULF1 | sulfatase 1 | XM_024447112.1 | 99.9% | 99.8% | 2613_2614insG |
| 6 | human | 23213 | SULF1 | sulfatase 1 | XM_011517495.2 | 90.6% | 86.8% | 2284_2285ins143;2370_2371ins101 |
| 7 | human | 23213 | SULF1 | sulfatase 1 | XM_017013250.2 | 90.6% | 86.8% | 2284_2285ins143;2370_2371ins101 |
| 8 | human | 23213 | SULF1 | sulfatase 1 | XM_006716438.3 | 75.8% | 76.2% | 2550_2551ins34;2580_3366delinsG |
| 9 | human | 23213 | SULF1 | sulfatase 1 | XM_006716439.3 | 75.8% | 76.2% | 2550_2551ins34;2580_3366delinsG |
| 10 | human | 23213 | SULF1 | sulfatase 1 | XM_006716440.3 | 75.8% | 76.2% | 2550_2551ins34;2580_3366delinsG |
| 11 | human | 23213 | SULF1 | sulfatase 1 | XM_011517494.2 | 75.8% | 76.2% | 2550_2551ins34;2580_3366delinsG |
| 12 | human | 23213 | SULF1 | sulfatase 1 | XM_006716442.1 | 72% | 71.7% | (many diffs) |
| 13 | human | 23213 | SULF1 | sulfatase 1 | XM_006716441.1 | 54.4% | 54.3% | (many diffs) |
| 14 | human | 23213 | SULF1 | sulfatase 1 | NR_156414.2 | 44.9% | 1_507del;2791_2792ins143;2978_5357delinsG | |
| 15 | human | 23213 | SULF1 | sulfatase 1 | NR_156415.2 | 43.6% | 1_669del;2953_2954ins143;3140_5519delinsG | |
| 16 | human | 23213 | SULF1 | sulfatase 1 | NR_132437.1 | 37.5% | (many diffs) | |
| 17 | mouse | 240725 | Sulf1 | sulfatase 1 | NM_001198565.1 | 86.1% | 92.7% | (many diffs) |
| 18 | mouse | 240725 | Sulf1 | sulfatase 1 | NM_001198566.1 | 86.1% | 92.7% | (many diffs) |
| 19 | mouse | 240725 | Sulf1 | sulfatase 1 | NM_172294.1 | 86.1% | 92.7% | (many diffs) |
| 20 | mouse | 240725 | Sulf1 | sulfatase 1 | XM_006495510.3 | 65.4% | 70.8% | (many diffs) |
| 21 | mouse | 240725 | Sulf1 | sulfatase 1 | XM_006495511.3 | 65.4% | 70.8% | (many diffs) |
| 22 | mouse | 240725 | Sulf1 | sulfatase 1 | XM_006495512.3 | 65.4% | 70.8% | (many diffs) |
| 23 | mouse | 240725 | Sulf1 | sulfatase 1 | XM_006495513.3 | 65.4% | 70.8% | (many diffs) |
| 24 | mouse | 240725 | Sulf1 | sulfatase 1 | XM_006495514.1 | 65.4% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2682
- ORF length:
- 2616
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatgaa gtattcttgc tgtgctctgg ttttggctgt cctgggcaca gaattgctgg 121 gaagcctctg ttcgactgtc agatccccga ggttcagagg acggatacag caggaacgaa 181 aaaacatccg acccaacatt attcttgtgc ttaccgatga tcaagatgtg gagctggggt 241 ccctgcaagt catgaacaaa acgagaaaga ttatggaaca tgggggggcc accttcatca 301 atgcctttgt gactacaccc atgtgctgcc cgtcacggtc ctccatgctc accgggaagt 361 atgtgcacaa tcacaatgtc tacaccaaca acgagaactg ctcttccccc tcgtggcagg 421 ccatgcatga gcctcggact tttgctgtat atcttaacaa cactggctac agaacagcct 481 tttttggaaa atacctcaat gaatataatg gcagctacat cccccctggg tggcgagaat 541 ggcttggatt aatcaagaat tctcgcttct ataattacac tgtttgtcgc aatggcatca 601 aagaaaagca tggatttgat tatgcaaagg actacttcac agacttaatc actaacgaga 661 gcattaatta cttcaaaatg tctaagagaa tgtatcccca taggcccgtt atgatggtga 721 tcagccacgc tgcgccccac ggccccgagg actcagcccc acagttttct aaactgtacc 781 ccaatgcttc ccaacacata actcctagtt ataactatgc accaaatatg gataaacact 841 ggattatgca gtacacagga ccaatgctgc ccatccacat ggaatttaca aacattctac 901 agcgcaaaag gctccagact ttgatgtcag tggatgattc tgtggagagg ctgtataaca 961 tgctcgtgga gacgggggag ctggagaata cttacatcat ttacaccgcc gaccatggtt 1021 accatattgg gcagtttgga ctggtcaagg ggaaatccat gccatatgac tttgatattc 1081 gtgtgccttt ttttattcgt ggtccaagtg tagaaccagg atcaatagtc ccacagatcg 1141 ttctcaacat tgacttggcc cccacgatcc tggatattgc tgggctcgac acacctcctg 1201 atgtggacgg caagtctgtc ctcaaacttc tggacccaga aaagccaggt aacaggtttc 1261 gaacaaacaa gaaggccaaa atttggcgtg atacattcct agtggaaaga ggcaaatttc 1321 tacgtaagaa ggaagaatcc agcaagaata tccaacagtc aaatcacttg cccaaatatg 1381 aacgggtcaa agaactatgc cagcaggcca ggtaccagac agcctgtgaa caaccggggc 1441 agaagtggca atgcattgag gatacatctg gcaagcttcg aattcacaag tgtaaaggac 1501 ccagtgacct gctcacagtc cggcagagca cgcggaacct ctacgctcgc ggcttccatg 1561 acaaagacaa agagtgcagt tgtagggagt ctggttaccg tgccagcaga agccaaagaa 1621 agagtcaacg gcaattcttg agaaaccagg ggactccaaa gtacaagccc agatttgtcc 1681 atactcggca gacacgttcc ttgtccgtcg aatttgaagg tgaaatatat gacataaatc 1741 tggaagaaga agaagaattg caagtgttgc aaccaagaaa cattgctaag cgtcatgatg 1801 aaggccacaa ggggccaaga gatctccagg cttccagtgg tggcaacagg ggcaggatgc 1861 tggcagatag cagcaacgcc gtgggcccac ctaccactgt ccgagtgaca cacaagtgtt 1921 ttattcttcc caatgactct atccattgtg agagagaact gtaccaatcg gccagagcgt 1981 ggaaggacca taaggcatac attgacaaag agattgaagc tctgcaagat aaaattaaga 2041 atttaagaga agtgagagga catctgaaga gaaggaagcc tgaggaatgt agctgcagta 2101 aacaaagcta ttacaataaa gagaaaggtg taaaaaagca agagaaatta aagagccatc 2161 ttcacccatt caaggaggct gctcaggaag tagatagcaa actgcaactt ttcaaggaga 2221 acaaccgtag gaggaagaag gagaggaagg agaagagacg gcagaggaag ggggaagagt 2281 gcagcctgcc tggCCTCACT TGCTTCACGC ATGACAACAA CCACTGGCAG ACAGCCCCGT 2341 TCTGGAACCT GGGATCTTTC TGTGCTTGCA CGAGTTCTAA CAATAACACC TACTGGTGTT 2401 TGCGTACAGT TAATGAGACG CATAATTTTC TTTTCTGTGA GTTTGCTACT GGCTTTTTGG 2461 AGTATTTTGA TATGAATACA GATCCTTATC AGCTCACAAA TACAGTGCAC ACGGTAGAAC 2521 GAGGCATTTT GAATCAGCTA CACGTACAAC TAATGGAGCT CAGAAGCTGT CAAGGATATA 2581 AGCAGTGCAA CCCAAGACCT AAGAATCTTG ATGTTGGAAA TAAAGATGGA GGAAGCTATG 2641 ACCTACACAG AGGACAGTTA TGGGATGGAT GGGAAGGTGA CCCAGCTTTC TTGTACAAAG 2701 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2761 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2821 ACGACCCGTC CTGAATGCGC ACCTCTTCAC GCGTTAAGTC gacaatcaac ctctggatta 2881 caaaatttgt gaaagatt