Construct: ORF TRCN0000489541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021971.1_s317c1
- DNA Barcode:
- AAAGCGGGAAGACGTATTGCCAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPARG (5468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5468 | PPARG | peroxisome proliferator act... | NM_015869.5 | 99.8% | 99.8% | 817T>G;1431C>T |
| 2 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354666.2 | 94.3% | 94.2% | 0_1ins84;733T>G;1347C>T |
| 3 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354667.2 | 94.3% | 94.2% | 0_1ins84;733T>G;1347C>T |
| 4 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374263.1 | 94.3% | 94.2% | 0_1ins84;733T>G;1347C>T |
| 5 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374264.1 | 94.3% | 94.2% | 0_1ins84;733T>G;1347C>T |
| 6 | human | 5468 | PPARG | peroxisome proliferator act... | NM_005037.6 | 94.3% | 94.2% | 0_1ins84;733T>G;1347C>T |
| 7 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138711.4 | 94.3% | 94.2% | 0_1ins84;733T>G;1347C>T |
| 8 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138712.4 | 94.3% | 94.2% | 0_1ins84;733T>G;1347C>T |
| 9 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354668.2 | 57.1% | 54.5% | (many diffs) |
| 10 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374265.1 | 54.2% | 53.8% | (many diffs) |
| 11 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354669.2 | 52.6% | 45.1% | 0_1ins517;102_103ins200;714C>T |
| 12 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354670.2 | 51.6% | 49% | (many diffs) |
| 13 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001330615.2 | 48.7% | 48.3% | (many diffs) |
| 14 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374261.1 | 48.7% | 48.3% | (many diffs) |
| 15 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374262.1 | 48.7% | 48.3% | (many diffs) |
| 16 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374266.1 | 43.8% | 43.7% | (many diffs) |
| 17 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_011146.3 | 89.9% | 96% | (many diffs) |
| 18 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505737.3 | 87.7% | 92.7% | (many diffs) |
| 19 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505738.3 | 87.7% | 92.7% | (many diffs) |
| 20 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505739.2 | 87.7% | 92.7% | (many diffs) |
| 21 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001127330.2 | 85.1% | 92.2% | (many diffs) |
| 22 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308352.1 | 85.1% | 92.2% | (many diffs) |
| 23 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308354.1 | 85.1% | 92.2% | (many diffs) |
| 24 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505743.3 | 85.1% | 92.2% | (many diffs) |
| 25 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_011241252.1 | 85.1% | 92.2% | (many diffs) |
| 26 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321455.1 | 85.1% | 92.2% | (many diffs) |
| 27 | mouse | 19016 | Pparg | peroxisome proliferator act... | XR_001785108.1 | 64.9% | (many diffs) | |
| 28 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321456.1 | 52.6% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1584
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggtgaaact ctgggagatt ctcctattga cccagaaagc gattccttca 121 ctgatacact gtctgcaaac atatcacaag aaatgaccat ggttgacaca gagatgccat 181 tctggcccac caactttggg atcagctccg tggatctctc cgtaatggaa gaccactccc 241 actcctttga tatcaagccc ttcactactg ttgacttctc cagcatttct actccacatt 301 acgaagacat tccattcaca agaacagatc cagtggttgc agattacaag tatgacctga 361 aacttcaaga gtaccaaagt gcaatcaaag tggagcctgc atctccacct tattattctg 421 agaagactca gctctacaat aagcctcatg aagagccttc caactccctc atggcaattg 481 aatgtcgtgt ctgtggagat aaagcttctg gatttcacta tggagttcat gcttgtgaag 541 gatgcaaggg tttcttccgg agaacaatca gattgaagct tatctatgac agatgtgatc 601 ttaactgtcg gatccacaaa aaaagtagaa ataaatgtca gtactgtcgg tttcagaaat 661 gccttgcagt ggggatgtct cataatgcca tcaggtttgg gcggatgcca caggccgaga 721 aggagaagct gttggcggag atctccagtg atatcgacca gctgaatcca gagtccgctg 781 acctccgggc cctggcaaaa catttgtatg actcatacat aaagtccttc ccgctgacca 841 aagcaaaggc gagggcgatc ttgacaggaa agacaacaga caaagcacca ttcgttatct 901 atgacatgaa ttccttaatg atgggagaag ataaaatcaa gttcaaacac atcacccccc 961 tgcaggagca gagcaaagag gtggccatcc gcatctttca gggctgccag tttcgctccg 1021 tggaggctgt gcaggagatc acagagtatg ccaaaagcat tcctggtttt gtaaatcttg 1081 acttgaacga ccaagtaact ctcctcaaat atggagtcca cgagatcatt tacacaatgc 1141 tggcctcctt gatgaataaa gatggggttc tcatatccga gggccaaggc ttcatgacaa 1201 gggagtttct aaagagcctg cgaaagcctt ttggtgactt tatggagccC AAGTTTGAGT 1261 TTGCTGTGAA GTTCAATGCA CTGGAATTAG ATGACAGCGA CTTGGCAATA TTTATTGCTG 1321 TCATTATTCT CAGTGGAGAC CGCCCAGGTT TGCTGAATGT GAAGCCCATT GAAGACATTC 1381 AAGACAACCT GCTACAAGCC CTGGAGCTCC AGCTGAAGCT GAACCACCCT GAGTCCTCAC 1441 AGCTGTTTGC CAAGCTGCTC CAGAAAATGA CAGACCTCAG ACAGATTGTC ACGGAACATG 1501 TGCAGCTACT GCAGGTGATC AAGAAGACGG AGACAGACAT GAGTCTTCAC CCGCTCCTGC 1561 AGGAGATCTA CAAGGACTTG TACTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1621 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1681 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAAGCGGG 1741 AAGACGTATT GCCAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1801 aagatt