Construct: ORF TRCN0000489541

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021971.1_s317c1
DNA Barcode:
AAAGCGGGAAGACGTATTGCCAGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PPARG (5468)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489541
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5468 PPARG peroxisome proliferator act... NM_015869.5 99.8% 99.8% 817T>G;1431C>T
2 human 5468 PPARG peroxisome proliferator act... NM_001354666.2 94.3% 94.2% 0_1ins84;733T>G;1347C>T
3 human 5468 PPARG peroxisome proliferator act... NM_001354667.2 94.3% 94.2% 0_1ins84;733T>G;1347C>T
4 human 5468 PPARG peroxisome proliferator act... NM_001374263.1 94.3% 94.2% 0_1ins84;733T>G;1347C>T
5 human 5468 PPARG peroxisome proliferator act... NM_001374264.1 94.3% 94.2% 0_1ins84;733T>G;1347C>T
6 human 5468 PPARG peroxisome proliferator act... NM_005037.6 94.3% 94.2% 0_1ins84;733T>G;1347C>T
7 human 5468 PPARG peroxisome proliferator act... NM_138711.4 94.3% 94.2% 0_1ins84;733T>G;1347C>T
8 human 5468 PPARG peroxisome proliferator act... NM_138712.4 94.3% 94.2% 0_1ins84;733T>G;1347C>T
9 human 5468 PPARG peroxisome proliferator act... NM_001354668.2 57.1% 54.5% (many diffs)
10 human 5468 PPARG peroxisome proliferator act... NM_001374265.1 54.2% 53.8% (many diffs)
11 human 5468 PPARG peroxisome proliferator act... NM_001354669.2 52.6% 45.1% 0_1ins517;102_103ins200;714C>T
12 human 5468 PPARG peroxisome proliferator act... NM_001354670.2 51.6% 49% (many diffs)
13 human 5468 PPARG peroxisome proliferator act... NM_001330615.2 48.7% 48.3% (many diffs)
14 human 5468 PPARG peroxisome proliferator act... NM_001374261.1 48.7% 48.3% (many diffs)
15 human 5468 PPARG peroxisome proliferator act... NM_001374262.1 48.7% 48.3% (many diffs)
16 human 5468 PPARG peroxisome proliferator act... NM_001374266.1 43.8% 43.7% (many diffs)
17 mouse 19016 Pparg peroxisome proliferator act... NM_011146.3 89.9% 96% (many diffs)
18 mouse 19016 Pparg peroxisome proliferator act... XM_006505737.3 87.7% 92.7% (many diffs)
19 mouse 19016 Pparg peroxisome proliferator act... XM_006505738.3 87.7% 92.7% (many diffs)
20 mouse 19016 Pparg peroxisome proliferator act... XM_006505739.2 87.7% 92.7% (many diffs)
21 mouse 19016 Pparg peroxisome proliferator act... NM_001127330.2 85.1% 92.2% (many diffs)
22 mouse 19016 Pparg peroxisome proliferator act... NM_001308352.1 85.1% 92.2% (many diffs)
23 mouse 19016 Pparg peroxisome proliferator act... NM_001308354.1 85.1% 92.2% (many diffs)
24 mouse 19016 Pparg peroxisome proliferator act... XM_006505743.3 85.1% 92.2% (many diffs)
25 mouse 19016 Pparg peroxisome proliferator act... XM_011241252.1 85.1% 92.2% (many diffs)
26 mouse 19016 Pparg peroxisome proliferator act... XM_017321455.1 85.1% 92.2% (many diffs)
27 mouse 19016 Pparg peroxisome proliferator act... XR_001785108.1 64.9% (many diffs)
28 mouse 19016 Pparg peroxisome proliferator act... XM_017321456.1 52.6% 57% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1584
ORF length:
1515
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gggtgaaact ctgggagatt ctcctattga cccagaaagc gattccttca
121ctgatacact gtctgcaaac atatcacaag aaatgaccat ggttgacaca gagatgccat
181tctggcccac caactttggg atcagctccg tggatctctc cgtaatggaa gaccactccc
241actcctttga tatcaagccc ttcactactg ttgacttctc cagcatttct actccacatt
301acgaagacat tccattcaca agaacagatc cagtggttgc agattacaag tatgacctga
361aacttcaaga gtaccaaagt gcaatcaaag tggagcctgc atctccacct tattattctg
421agaagactca gctctacaat aagcctcatg aagagccttc caactccctc atggcaattg
481aatgtcgtgt ctgtggagat aaagcttctg gatttcacta tggagttcat gcttgtgaag
541gatgcaaggg tttcttccgg agaacaatca gattgaagct tatctatgac agatgtgatc
601ttaactgtcg gatccacaaa aaaagtagaa ataaatgtca gtactgtcgg tttcagaaat
661gccttgcagt ggggatgtct cataatgcca tcaggtttgg gcggatgcca caggccgaga
721aggagaagct gttggcggag atctccagtg atatcgacca gctgaatcca gagtccgctg
781acctccgggc cctggcaaaa catttgtatg actcatacat aaagtccttc ccgctgacca
841aagcaaaggc gagggcgatc ttgacaggaa agacaacaga caaagcacca ttcgttatct
901atgacatgaa ttccttaatg atgggagaag ataaaatcaa gttcaaacac atcacccccc
961tgcaggagca gagcaaagag gtggccatcc gcatctttca gggctgccag tttcgctccg
1021tggaggctgt gcaggagatc acagagtatg ccaaaagcat tcctggtttt gtaaatcttg
1081acttgaacga ccaagtaact ctcctcaaat atggagtcca cgagatcatt tacacaatgc
1141tggcctcctt gatgaataaa gatggggttc tcatatccga gggccaaggc ttcatgacaa
1201gggagtttct aaagagcctg cgaaagcctt ttggtgactt tatggagccC AAGTTTGAGT
1261TTGCTGTGAA GTTCAATGCA CTGGAATTAG ATGACAGCGA CTTGGCAATA TTTATTGCTG
1321TCATTATTCT CAGTGGAGAC CGCCCAGGTT TGCTGAATGT GAAGCCCATT GAAGACATTC
1381AAGACAACCT GCTACAAGCC CTGGAGCTCC AGCTGAAGCT GAACCACCCT GAGTCCTCAC
1441AGCTGTTTGC CAAGCTGCTC CAGAAAATGA CAGACCTCAG ACAGATTGTC ACGGAACATG
1501TGCAGCTACT GCAGGTGATC AAGAAGACGG AGACAGACAT GAGTCTTCAC CCGCTCCTGC
1561AGGAGATCTA CAAGGACTTG TACTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1621GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1681AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAAGCGGG
1741AAGACGTATT GCCAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1801aagatt

Download FASTA (ORF) (Full)