Construct: ORF TRCN0000489584
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019957.1_s317c1
- DNA Barcode:
- CTCCCCAAGTGTGCGCTCTTCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR1D2 (9975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489584
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NM_005126.5 | 99.7% | 99.3% | (many diffs) |
| 2 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NM_001145425.1 | 86.8% | 86.5% | (many diffs) |
| 3 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | XM_006713451.3 | 77% | 76% | (many diffs) |
| 4 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NR_110524.2 | 32.4% | (many diffs) | |
| 5 | mouse | 353187 | Nr1d2 | nuclear receptor subfamily ... | NM_011584.4 | 88.3% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1812
- ORF length:
- 1740
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggtg aatgcaggag gtgtgattgc ctatatcagt tcttccagct 121 cagcctcaag ccatgcctct tgtcacagtg agggttctga gaatagtttc cagtcctcct 181 cctcttctgt tccatcttct ccaaatagct ctaattctga taccaatggt aatcccaaga 241 atggtgatct cgccaatatt gaaggcatct tgaagaatga tcgaatagat tgttctatga 301 aaacaagcaa atcgagtgca cctgggatga caaaaagtca tagtggtgtg acaaaattta 361 gtggcatggt tctactgtgt aaagtctgtg gggatgtggc gtcaggattc cactatggag 421 ttcatgcttg cgaaggctgt aagggtttct ttcggagaag tattcaacaa aacatccagt 481 acaagaagtg cctgaagaat gaaaactgtt ctataatgag aatgaatagg aacagatgtc 541 agcaatgtcg cttcaaaaag tgtctgtctg ttggaatgtc aagagatgct gttcggtttg 601 gtcgtattcc taagcgtgaa aaacagagga tgctaattga aatgcaaagt gcaatgaaga 661 ccatgatgaa cagccagttc agtggtcact tgcaaaatga cacattagta gaacatcatg 721 aacagacagc cttgccagcc caggaacagc tgcgacccaa gccccaactg gagcaagaaa 781 acatcaaaag ctcttctcct ccatcttctg attttgcaaa ggaagaagtg attggcatgg 841 tgaccagagc tcacaaggat acctttatgt ataatcaaga gcagcaagaa aactcagctg 901 agagcatgca gcccaagaga ggagaacgga ttcgcaagaa catggagcaa tataatttaa 961 atcatgatca ttgcggcaat gggcttagca gccattttcc ctgtagtgag agccagcagc 1021 atctcaatgg acagttcaaa gggaggaata taatgcatta cccaaatggt catgccattt 1081 gtattgcaaa tggacattgt atgaacttct ccaatgctta tactcaaaga gtatgtgata 1141 gagttccgat agatggattt tctcagaatg agaacaagaa tagttacctg tgcaacactg 1201 gaggaagaat gcatctggtt tgtccaatga gtaagtctcc atatgtggat cctcataaat 1261 caggacatga aatctgggaa gaattttcga tgagcttcac tccagcagtg aaagaagtgg 1321 tggaatttgc aaagcgtatt cctgggttca gagatctctc tcagcatgac caggtcaacc 1381 ttttaaaggc tgggactttt gaggttttaa tggtacggtt cgcatcatta tttgatgcaa 1441 aggaacgtac tgtcaccttt ttaagtggaa agaaatatag tgtggatgat ttacactcaa 1501 tGGGAGCAGG GGATCTGCTA AACTCTATGT TTGAATTTAG TGAGAAGCTA AATGCCCTCC 1561 AACTTAGTGA TGAAGAGATG AGTTTGTTTA CAGCTGTTGT CCTGGTATCT GCAGATCGAT 1621 CTGGAATAGA AAACGTCAAC TCTGTGGAGG CTTTGCAGGA AACTCTCATT CGTGCACTAA 1681 GGACCTTAAT AATGAAAAAC CATCCAAATG AGGCCTCTAT TTTTACAAAA CTGCTTCTAA 1741 AGTTGCCAGA TCTTCGATCT TTAAACAACA TGCACTCTGA GGAGCTCTTG GCCTTTAAAG 1801 TTCACCCTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1861 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1921 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCCCC AAGTGTGCGC TCTTCCGAAC 1981 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt