Construct: ORF TRCN0000489587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019559.1_s317c1
- DNA Barcode:
- TTCCGACATTAAGCCGCAACCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489587
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 99.9% | 100% | 2361G>A;2709T>C |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2239_2247del | 99.7% | 99.7% | 2235_2236insGAATTAAGA;2352G>A |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 99.5% | 99.5% | 2237_2238insATTAAGAGAAGCAAC;2346G>A |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 96.2% | 96.1% | 424_425ins135;2226G>A;2574T>C |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 95.5% | 95.6% | 0_1ins159;2202G>A;2550T>C |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 92.5% | 90.3% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 88.8% | 86.6% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 77.3% | 75.1% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.5% | 50.7% | (many diffs) |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 51.8% | 51.8% | 1881G>A;1883C>G;1884_1885ins1746 |
| 11 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 33.4% | 33.3% | 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2414 |
| 12 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 84.7% | 90.3% | (many diffs) |
| 13 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 44.9% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 3723
- ORF length:
- 3630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcacctct agaggagcag cgatgcgacc ctccgggacg gccggggcag 121 cgctcctggc gctgctggct gcgctctgcc cggcgagtcg ggctctggag gaaaagaaag 181 tttgccaagg cacgagtaac aagctcacgc agttgggcac ttttgaagat cattttctca 241 gcctccagag gatgttcaat aactgtgagg tggtccttgg gaatttggaa attacctatg 301 tgcagaggaa ttatgatctt tccttcttaa agaccatcca ggaggtggct ggttatgtcc 361 tcattgccct caacacagtg gagcgaattc ctttggaaaa cctgcagatc atcagaggaa 421 atatgtacta cgaaaattcc tatgccttag cagtcttatc taactatgat gcaaataaaa 481 ccggactgaa ggagctgccc atgagaaatt tacaggaaat cctgcatggc gccgtgcggt 541 tcagcaacaa ccctgccctg tgcaacgtgg agagcatcca gtggcgggac atagtcagca 601 gtgactttct cagcaacatg tcgatggact tccagaacca cctgggcagc tgccaaaagt 661 gtgatccaag ctgtcccaat gggagctgct ggggtgcagg agaggagaac tgccagaaac 721 tgaccaaaat catctgtgcc cagcagtgct ccgggcgctg ccgtggcaag tcccccagtg 781 actgctgcca caaccagtgt gctgcaggct gcacaggccc ccgggagagc gactgcctgg 841 tctgccgcaa attccgagac gaagccacgt gcaaggacac ctgcccccca ctcatgctct 901 acaaccccac cacgtaccag atggatgtga accccgaggg caaatacagc tttggtgcca 961 cctgcgtgaa gaagtgtccc cgtaattatg tggtgacaga tcacggctcg tgcgtccgag 1021 cctgtggggc cgacagctat gagatggagg aagacggcgt ccgcaagtgt aagaagtgcg 1081 aagggccttg ccgcaaagtg tgtaacggaa taggtattgg tgaatttaaa gactcactct 1141 ccataaatgc tacgaatatt aaacacttca aaaactgcac ctccatcagt ggcgatctcc 1201 acatcctgcc ggtggcattt aggggtgact ccttcacaca tactcctcct ctggatccac 1261 aggaactgga tattctgaaa accgtaaagg aaatcacagg gtttttgctg attcaggctt 1321 ggcctgaaaa caggacggac ctccatgcct ttgagaacct agaaatcata cgcggcagga 1381 ccaagcaaca tggtcagttt tctcttgcag tcgtcagcct gaacataaca tccttgggat 1441 tacgctccct caaggagata agtgatggag atgtgataat ttcaggaaac aaaaatttgt 1501 gctatgcaaa tacaataaac tggaaaaaac tgtttgggac ctccggtcag aaaaccaaaa 1561 ttataagcaa cagaggtgaa aacagctgca aggccacagg ccaggtctgc catgccttgt 1621 gctcccccga gggctgctgg ggcccggagc ccagggactg cgtctcttgc cggaatgtca 1681 gccgaggcag ggaatgcgtg gacaagtgca accttctgga gggtgagcca agggagtttg 1741 tggagaactc tgagtgcata cagtgccacc cagagtgcct gcctcaggcc atgaacatca 1801 cctgcacagg acggggacca gacaactgta tccagtgtgc ccactacatt gacggccccc 1861 actgcgtcaa gacctgcccg gcaggagtca tgggagaaaa caacaccctg gtctggaagt 1921 acgcagacgc cggccatgtg tgccacctgt gccatccaaa ctgcacctac ggatgcactg 1981 ggccaggtct tgaaggctgt ccaacgaatg ggcctaagat cccgtccatc gccactggga 2041 tggtgggggc cctcctcttg ctgctggtgg tggccctggg gatcggcctc ttcatgcgaa 2101 ggcgccacat cgttcggaag cgcacgctgc ggaggctgct gcaggagagg gagcttgtgg 2161 agcctcttac acccagtgga gaagctccca accaagctct cttgaggatc ttgaaggaaa 2221 ctgaattcaa aaagatcaaa gtgctgggct ccggtgcgtt cggcacggtg tataagggac 2281 tctggatccc agaaggtgag aaagttaaaa ttcccgtcgc tatcaaggaa ttaagagaag 2341 caacatctcc gaaagccaac aaggaaatcc tcgatgaagc ctacgtgatg gccagcgtgg 2401 acaaccccca cgtgtgccgc ctgctgggca tctgcctcac ctccaccgtg caactcatca 2461 cgcagctcat gcccttcggc tgcctcctgg actatgtccg ggaacacaaa gacaatattg 2521 gctcccagta cctgctcaac tggtgtgtgc agatcgcaaa gggcatgaac tacttggagg 2581 accgtcgctt ggtgcaccgc gacctggcag ccaggaacgt actggtgaaa acaccgcagc 2641 atgtcaagat cacagatttt gggctggcca aactgctggg tgcggaagag aaagaatacc 2701 atgcagaagg aggcaaagtg cctatcaagt ggatggcatt ggaatcaatt ttacacagaa 2761 tctataccca ccagagtgat gtctggagct acggggtgac cgtttgggag ttgatgacct 2821 ttggatccaa gccatatgac ggaatccctg ccagcgagat ctcctccatc ctggagaaag 2881 gagaacgcct ccctcagcca cccatatgta ccatcgatgt ctacatgatc atggtcaagt 2941 gctggatgat agacgcagat agtcgcccaa agttccgtga gttgatcatc gaattctcca 3001 aaatggcccg agacccccag cgctaccttg tcattcaggg ggatgaaaga atgcatttgc 3061 caagtcctac agactccaac ttctaccgtg ccctgatgga tgaagaagac atggacgacg 3121 tggtggatgc cgacgagtac ctcatcccac agcagggctt cttcagcagc ccctccacgt 3181 cacggactcc cctcctgagc tctctgagtg caaccagcaa caattccacc gtggcttgca 3241 ttgatagaaa tgggctgcaa agctgtccca tcaaggaaga cagcttcttg cagcgataca 3301 gctcagaccc cacaggcgCC TTGACTGAGG ACAGCATAGA CGACACCTTC CTCCCAGTGC 3361 CTGAATACAT AAACCAGTCC GTTCCCAAAA GGCCCGCTGG CTCTGTGCAG AATCCTGTCT 3421 ATCACAATCA GCCTCTGAAC CCCGCGCCCA GCAGAGACCC ACACTACCAG GACCCCCACA 3481 GCACTGCAGT GGGCAACCCC GAGTATCTCA ACACTGTCCA GCCCACCTGT GTCAACAGCA 3541 CATTCGACAG CCCTGCCCAC TGGGCCCAGA AAGGCAGCCA CCAAATTAGC CTGGACAACC 3601 CTGACTACCA GCAGGACTTC TTTCCCAAGG AAGCCAAGCC AAATGGCATC TTTAAGGGCT 3661 CCACAGCTGA AAATGCAGAA TACCTAAGGG TCGCGCCACA AAGCAGTGAA TTTATTGGAG 3721 CAAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCCGAC ATTAAGCCGC 3901 AACCGAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt