Construct: ORF TRCN0000489654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019896.1_s317c1
- DNA Barcode:
- TTTCACGGAGCTGCGCAATGCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMURF1 (57154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57154 | SMURF1 | SMAD specific E3 ubiquitin ... | NM_181349.3 | 99.9% | 99.8% | 2193_2194insG |
| 2 | human | 57154 | SMURF1 | SMAD specific E3 ubiquitin ... | NM_001199847.2 | 99.5% | 99.4% | 2009_2010insAGGTTCTAC;2184_2185insG |
| 3 | human | 57154 | SMURF1 | SMAD specific E3 ubiquitin ... | NM_020429.3 | 96.5% | 96.4% | 805_882del;2271_2272insG |
| 4 | human | 57154 | SMURF1 | SMAD specific E3 ubiquitin ... | XM_017012457.1 | 96.1% | 96% | 805_882del;2087_2088insAGGTTCTAC;2262_2263insG |
| 5 | mouse | 75788 | Smurf1 | SMAD specific E3 ubiquitin ... | NM_001038627.1 | 88.1% | 98.4% | (many diffs) |
| 6 | mouse | 75788 | Smurf1 | SMAD specific E3 ubiquitin ... | NM_029438.3 | 87.8% | 98% | (many diffs) |
| 7 | mouse | 75788 | Smurf1 | SMAD specific E3 ubiquitin ... | XM_006504884.1 | 85.2% | 95.1% | (many diffs) |
| 8 | mouse | 75788 | Smurf1 | SMAD specific E3 ubiquitin ... | XM_011241016.1 | 84.9% | 94.7% | (many diffs) |
| 9 | mouse | 75788 | Smurf1 | SMAD specific E3 ubiquitin ... | XM_011241017.1 | 82.3% | 91.6% | (many diffs) |
| 10 | mouse | 75788 | Smurf1 | SMAD specific E3 ubiquitin ... | XM_006504888.3 | 64.2% | 72.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2265
- ORF length:
- 2196
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtcgaacccc gggacacgca ggaacggctc cagcatcaag atccgtctga 121 cagtgttatg tgccaagaac cttgcaaaga aagacttctt caggctccct gacccttttg 181 caaagattgt cgtggatggg tctgggcagt gccactcaac cgacactgtg aaaaacacat 241 tggacccaaa gtggaaccag cactatgatc tatatgttgg gaaaacggat tcgataacca 301 ttagcgtgtg gaaccataag aaaattcaca agaaacaggg agctggcttc ctgggctgtg 361 tgcggctgct ctccaatgcc atcagcagat taaaagatac cggataccag cgtttggatc 421 tatgcaaact aaacccctca gatactgatg cagttcgtgg ccagatagtg gtcagtttac 481 agacacgaga cagaatagga accggcggct cggtggtgga ctgcagagga ctgttagaaa 541 atgaaggaac ggtgtatgaa gactccgggc ctgggaggcc gctcagctgc ttcatggagg 601 aaccagcccc ttacacagat agcaccggtg ctgctgctgg aggagggaat tgcaggttcg 661 tggagtcccc aagtcaagat caaagacttc aggcacagcg gcttcgaaac cctgatgtgc 721 gaggttcact acagacgccc cagaaccgac cacacggcca ccagtccccg gaactgcccg 781 aaggctacga acaaagaaca acagtccagg gccaagttta ctttttgcat acacagactg 841 gagttagcac gtggcacgac cccaggatac caagagacct taacagtgtg aactgtgatg 901 aacttggacc actgccgcca ggctgggaag tcagaagtac agtttctggg aggatatatt 961 ttgtagatca taataaccga acaacccagt ttacagaccc aaggttacac cacatcatga 1021 atcaccagtg ccaactcaag gagcccagcc agccgctgcc actgcccagt gagggctctc 1081 tggaggacga ggagcttcct gcccagagat acgaaagaga tctagtccag aagctgaaag 1141 tcctcagaca cgaactgtcg cttcagcagc cccaagctgg tcattgccgc atcgaagtgt 1201 ccagagaaga aatctttgag gagtcttacc gccagataat gaagatgcga ccgaaagact 1261 tgaaaaaacg gctgatggtg aaattccgtg gggaagaagg tttggattac ggtggtgtgg 1321 ccagggagtg gctttacttg ctgtgccatg aaatgctgaa tccttattac gggctcttcc 1381 agtattctac ggacaatatt tacatgttgc aaataaatcc ggattcttca atcaaccccg 1441 accacttgtc ttatttccac tttgtggggc ggatcatggg gctggctgtg ttccatggac 1501 actacatcaa cgggggcttc acagtgccct tctacaagca gctgctgggg aagcccatcc 1561 agctctcaga tctggaatct gtggacccag agctgcataa gagcttggtg tggatcctag 1621 agaacgacat cacgcctgta ctggaccaca ccttctgcgt ggaacacaac gccttcgggc 1681 ggatcctgca gcatgaactg aaacccaatg gcagaaatgt gccagtcaca gaggagaata 1741 agaaagaata cgtccggttg tatgtaaact ggaggtttat gagaggaatc gaagcccagt 1801 tcttagctct gcagaagggg ttcaatgagc tcatccctca acatctgctg aagccttttg 1861 accagaagga actGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG AACGACTGGA 1921 AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG TGGTTCTGGC 1981 AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT GTGACTGGGT 2041 CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC GCGGCAGGGC 2101 CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG AAGGCCCATA 2161 CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC TACGAGAAGC 2221 TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GGACCCAGCT TTCTTGTACA 2281 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2341 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2401 AGGACGATTT CACGGAGCTG CGCAATGCGC CACGCGTTAA GTCgacaatc aacctctgga 2461 ttacaaaatt tgtgaaagat t