Construct: ORF TRCN0000489736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020898.1_s317c1
- DNA Barcode:
- CATATCTTAGGAAGGAATTCTCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CCKBR (887)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 887 | CCKBR | cholecystokinin B receptor | NM_176875.4 | 99.9% | 99.7% | 617T>C |
| 2 | human | 887 | CCKBR | cholecystokinin B receptor | NM_001363552.2 | 86.5% | 86.4% | 617T>C;809_1015del |
| 3 | human | 887 | CCKBR | cholecystokinin B receptor | XM_017018516.1 | 85.1% | 85% | 0_1ins198;419T>C |
| 4 | human | 887 | CCKBR | cholecystokinin B receptor | NM_001318029.2 | 81.1% | 80.9% | 150_151ins252;365T>C |
| 5 | mouse | 12426 | Cckbr | cholecystokinin B receptor | NM_007627.5 | 83.9% | 87.6% | (many diffs) |
| 6 | mouse | 12426 | Cckbr | cholecystokinin B receptor | XM_006507280.3 | 83.8% | 87.4% | (many diffs) |
| 7 | mouse | 12426 | Cckbr | cholecystokinin B receptor | XM_006507281.3 | 48.2% | 50.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1413
- ORF length:
- 1341
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagctg ctaaagctga accggagcgt gcagggaacc ggacccgggc 121 cgggggcttc cctgtgccgc ccgggggcgc ctctcctcaa cagcagcagt gtgggcaacc 181 tcagctgcga gccccctcgc attcgcggag ccgggacacg agaattggag ctggccatta 241 gaatcactct ttacgcagtg atcttcctga tgagcgttgg aggaaatatg ctcatcatcg 301 tggtcctggg actgagccgc cgcctgagga ctgtcaccaa tgccttcctc ctctcactgg 361 cagtcagcga cctcctgctg gctgtggctt gcatgccctt caccctcctg cccaatctca 421 tgggcacatt catctttggc accgtcatct gcaaggcggt ttcctacctc atgggggtgt 481 ctgtgagtgt gtccacgcta agcctcgtgg ccatcgcact ggagcggtac agcgccatct 541 gccgaccact gcaggcacga gtgtggcaga cgcgctccca cgcggctcgc gtgattgtag 601 ccacgtggct gctgtccgga ctactcatgg tgccctaccc cgtgtacact gtcgtgcaac 661 cagtggggcc tcgtgtgctg cagtgcgcgc atcgctggcc cagtgcgcgg gtccgccaga 721 cctggtccgt actgctgctt ctgctcttgt tcttcatccc gggtgtggtt atggccgtgg 781 cctacgggct tatctctcgc gagctctact tagggcttcg ctttgacggc gacagtgaca 841 gcgacagcca aagcagggtc cgaaaccaag gcgggctgcc aggggctgtt caccagaacg 901 ggcgttgccg gcctgagact ggcgcggttg gcgaagacag cgatggctgc tacgtgcaac 961 ttccacgttc ccggcctgcc ctggagctga cggcgctgac ggctcctggg CCGGGATCCG 1021 GCTCCCGGCC CACCCAGGCC AAGCTGCTGG CTAAGAAGCG CGTGGTGCGA ATGTTGCTGG 1081 TGATCGTTGT GCTTTTTTTT CTGTGTTGGT TGCCAGTTTA TAGTGCCAAC ACGTGGCGCG 1141 CCTTTGATGG CCCGGGTGCA CACCGAGCAC TCTCGGGTGC TCCTATCTCC TTCATTCACT 1201 TGCTGAGCTA CGCCTCGGCC TGTGTCAACC CCCTGGTCTA CTGCTTCATG CACCGTCGCT 1261 TTCGCCAGGC CTGCCTGGAA ACTTGCGCTC GCTGCTGCCC CCGGCCTCCA CGAGCTCGCC 1321 CCAGGGCTCT TCCCGATGAG GACCCTCCCA CTCCCTCCAT TGCTTCGCTG TCCAGGCTTA 1381 GCTACACCAC CATCAGCACA CTGGGCCCTG GCTAGGACCC AGCTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 ACATATCTTA GGAAGGAATT CTCAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt