Transcript: Human XM_017018516.1

PREDICTED: Homo sapiens cholecystokinin B receptor (CCKBR), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CCKBR (887)
Length:
2669
CDS:
940..2085

Additional Resources:

NCBI RefSeq record:
XM_017018516.1
NBCI Gene record:
CCKBR (887)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018516.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005902 CGTCCCTAGCAGTGAACTATT pLKO.1 2340 3UTR 100% 13.200 18.480 N CCKBR n/a
2 TRCN0000356409 GTCCCTAGCAGTGAACTATTT pLKO_005 2341 3UTR 100% 13.200 18.480 N CCKBR n/a
3 TRCN0000368612 GTGTTGGTTGCCAGTTTATAG pLKO_005 1773 CDS 100% 13.200 18.480 N CCKBR n/a
4 TRCN0000005905 CACTCTTTACGCAGTGATCTT pLKO.1 915 5UTR 100% 4.950 6.930 N CCKBR n/a
5 TRCN0000356349 CTCTCACACACATAGATTAAT pLKO_005 2397 3UTR 100% 15.000 10.500 N CCKBR n/a
6 TRCN0000356410 GGCCATTAGAATCACTCTTTA pLKO_005 903 5UTR 100% 13.200 9.240 N CCKBR n/a
7 TRCN0000005904 GCTTCTGCTCTTGTTCTTCAT pLKO.1 1407 CDS 100% 4.950 3.465 N CCKBR n/a
8 TRCN0000005903 CGAATGTTGCTGGTGATCGTT pLKO.1 1738 CDS 100% 3.000 2.100 N CCKBR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018516.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05945 pDONR223 100% 85.1% 85.2% None 0_1ins198;225C>T n/a
2 ccsbBroad304_05945 pLX_304 0% 85.1% 85.2% V5 0_1ins198;225C>T n/a
3 TRCN0000467911 ACTGGCAAGCTCCACCCTGCATCA pLX_317 21.8% 85.1% 85.2% V5 0_1ins198;225C>T n/a
4 TRCN0000489736 CATATCTTAGGAAGGAATTCTCAA pLX_317 30.3% 85.1% 85% V5 (not translated due to prior stop codon) 0_1ins198;419T>C n/a
5 TRCN0000489791 GGGGCTCAAACATGTTCATGTGAC pLX_317 28.8% 85% 84.8% V5 0_1ins198;419T>C;1143_1144insG n/a
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