Construct: ORF TRCN0000489782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020385.1_s317c1
- DNA Barcode:
- CACACTTTCGCTGCCGTTTGCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KC1 (26750)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287220.2 | 78% | 78% | 1_396del |
| 2 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349663.1 | 78% | 78% | 1_396del |
| 3 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349664.1 | 78% | 78% | 1_396del |
| 4 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349665.1 | 78% | 78% | 1_396del |
| 5 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349666.1 | 78% | 78% | 1_396del |
| 6 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349667.1 | 78% | 78% | 1_396del |
| 7 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349668.1 | 78% | 78% | 1_396del |
| 8 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349669.1 | 78% | 78% | 1_396del |
| 9 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349670.1 | 78% | 78% | 1_396del |
| 10 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349671.1 | 78% | 78% | 1_396del |
| 11 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349672.1 | 73% | 73% | 1_396del;1424_1425ins90 |
| 12 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349659.1 | 65.6% | 65.6% | 1_735del |
| 13 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349660.1 | 65.6% | 65.6% | 1_735del |
| 14 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349661.1 | 65.6% | 65.6% | 1_735del |
| 15 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349662.1 | 65.6% | 65.6% | 1_735del |
| 16 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | XM_017001030.1 | 65.6% | 65.6% | 1_735del |
| 17 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349657.1 | 60.9% | 60.9% | 1_900del |
| 18 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349658.1 | 60.9% | 60.9% | 1_900del |
| 19 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287218.2 | 54.9% | 54.9% | 1_1155del |
| 20 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287219.2 | 54.9% | 54.9% | 1_1155del |
| 21 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349654.1 | 54.9% | 54.9% | 1_1155del |
| 22 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287221.2 | 52.9% | 52.9% | 1_1248del |
| 23 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349648.1 | 52.9% | 52.9% | 1_1248del |
| 24 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349649.1 | 52.9% | 52.9% | 1_1248del |
| 25 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349650.1 | 52.9% | 52.9% | 1_1248del |
| 26 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349651.1 | 52.9% | 52.9% | 1_1248del |
| 27 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349652.1 | 52.9% | 52.9% | 1_1248del |
| 28 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349653.1 | 52.9% | 52.9% | 1_1248del |
| 29 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349647.1 | 49.6% | 49.6% | 1_1428del |
| 30 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | XM_017001024.1 | 48.9% | 48.9% | 1_1248del;2548_2549delAGinsCT;2550_2551ins105 |
| 31 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349646.1 | 45.3% | 45.3% | 1_1698del |
| 32 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001136138.3 | 44.4% | 44.4% | 1_1755del |
| 33 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_012424.6 | 43.9% | 43.9% | 1_1791del |
| 34 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146208.1 | 26.5% | 1_1470del;2499_2769del;3149_5291del | |
| 35 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146209.1 | 25.1% | 1_1782del;2811_3081del;3461_5603del | |
| 36 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146207.1 | 24.3% | 1_1963del;2992_3262del;3642_5784del | |
| 37 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146210.1 | 23.8% | 1_2080del;3109_3379del;3759_5901del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1479
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaattc tttaggatag acagtaagga tagcgcaagt gaactcctgg 121 gacttgactt tggagaaaaa ttgtatagtc taaaatcaga acctttgaaa ccattcttta 181 ctcttccaga tggagacagt gcttctagga gttttaatac tagtgaaagc aaggtagagt 241 ttaaagctca ggacaccatt agcaggggct cagatgactc agtgccagtt atttcattta 301 aagatgctgc ttttgatgat gtcagtggta ctgatgaagg aagacctgat cttcttgtaa 361 atttacctgg tgaattggag tcaacaagag aagctgcagc aatgggacct actaagttta 421 cacaaactaa tatagggata atagaaaata aactcttgga agcccctgat gttttatgcc 481 tcaggcttag tactgaacaa tgccaagcac atgaggagaa aggcatagag gaactgagtg 541 atccctctgg gcccaaatcc tatagtataa cagagaaaca ctatgcacag gaggatccca 601 ggatgttatt tgtagcagct gttgatcata gtagttcagg agatatgtct ttgttaccca 661 gctcagatcc taagtttcaa ggacttggag tggttgagtc agcagtaact gcaaacaaca 721 cagaagaaag cttattccgt atttgtagtc cactctcagg tgctaatgaa tatattgcaa 781 gcacagacac tttaaaaaca gaagaagtat tgctgtttac agatcagact gatgatttgg 841 ctaaagagga accaacttct ttattccaga gagactctga gactaagggt gaaagtggtt 901 tagtgctaga aggagacaag gaaatacatc agatttttga ggaccttgat aaaaaattag 961 cactagcctc caggttttac atcccagagg gctgcattca aagatgggca gctgaaatgg 1021 tggtagccct tgatgcttta catagagagg gaattgtgtg ccgcgatttg aacccaaaca 1081 acatcttatt GAATGATAGA GGACACATTC AGCTAACGTA TTTTAGCAGG TGGAGTGAGG 1141 TTGAAGATTC CTGTGACAGC GATGCCATAG AGAGAATGTA CTGTGCCCCA GAGGTTGGAG 1201 CAATCACTGA AGAAACTGAA GCCTGTGATT GGTGGAGTTT GGGTGCTGTC CTCTTTGAAC 1261 TTCTCACTGG CAAGACTCTG GTTGAATGCC ATCCAGCAGG AATAAATACT CACACTACTT 1321 TGAACATGCC AGAATGTGTC TCTGAAGAGG CTCGCTCACT CATTCAACAG CTCTTGCAGT 1381 TCAATCCTCT GGAACGACTT GGTGCTGGAG TTGCTGGTGT TGAAGATATC AAATCTCATC 1441 CATTTTTTAC CCCTGTGGAT TGGGCAGAAC TGATGAGATA GGACCCAGCT TTCTTGTACA 1501 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1561 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1621 AGGACGACAC ACTTTCGCTG CCGTTTGCCT CACGCGTTAA GTCgacaatc aacctctgga 1681 ttacaaaatt tgtgaaagat t