Construct: ORF TRCN0000489791
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019579.1_s317c1
- DNA Barcode:
- GGGGCTCAAACATGTTCATGTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCKBR (887)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489791
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 887 | CCKBR | cholecystokinin B receptor | NM_176875.4 | 99.8% | 99.5% | 617T>C;1341_1342insG |
| 2 | human | 887 | CCKBR | cholecystokinin B receptor | NM_001363552.2 | 86.5% | 86.2% | 617T>C;809_1015del;1548_1549insG |
| 3 | human | 887 | CCKBR | cholecystokinin B receptor | XM_017018516.1 | 85% | 84.8% | 0_1ins198;419T>C;1143_1144insG |
| 4 | human | 887 | CCKBR | cholecystokinin B receptor | NM_001318029.2 | 81% | 80.8% | 150_151ins252;365T>C;1089_1090insG |
| 5 | mouse | 12426 | Cckbr | cholecystokinin B receptor | NM_007627.5 | 83.9% | 87.5% | (many diffs) |
| 6 | mouse | 12426 | Cckbr | cholecystokinin B receptor | XM_006507280.3 | 83.7% | 87.2% | (many diffs) |
| 7 | mouse | 12426 | Cckbr | cholecystokinin B receptor | XM_006507281.3 | 48.2% | 50.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1419
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag ctgctaaagc tgaaccggag cgtgcaggga accggacccg 121 ggccgggggc ttccctgtgc cgcccggggg cgcctctcct caacagcagc agtgtgggca 181 acctcagctg cgagccccct cgcattcgcg gagccgggac acgagaattg gagctggcca 241 ttagaatcac tctttacgca gtgatcttcc tgatgagcgt tggaggaaat atgctcatca 301 tcgtggtcct gggactgagc cgccgcctga ggactgtcac caatgccttc ctcctctcac 361 tggcagtcag cgacctcctg ctggctgtgg cttgcatgcc cttcaccctc ctgcccaatc 421 tcatgggcac attcatcttt ggcaccgtca tctgcaaggc ggtttcctac ctcatggggg 481 tgtctgtgag tgtgtccacg ctaagcctcg tggccatcgc actggagcgg tacagcgcca 541 tctgccgacc actgcaggca cgagtgtggc agacgcgctc ccacgcggct cgcgtgattg 601 tagccacgtg gctgctgtcc ggactactca tggtgcccta ccccgtgtac actgtcgtgc 661 aaccagtggg gcctcgtgtg ctgcagtgcg cgcatcgctg gcccagtgcg cgggtccgcc 721 agacctggtc cgtactgctg cttctgctct tgttcttcat cccgggtgtg gttatggccg 781 tggcctacgg gcttatctct cgcgagctct acttagggct tcgctttgac ggcgacagtg 841 acagcgacag ccaaagcagg gtccgaaacc aaggcgggct gccaggggct gttcaccaga 901 acgggcgttg ccggcctgag actggcgcgg ttggcgaaga cagcgatggc tgctacgtgc 961 aacttccacg ttcccggcct gccctggagc tgacggcgct gacggctcct gggccgggat 1021 ccggctcccg gCCCACCCAG GCCAAGCTGC TGGCTAAGAA GCGCGTGGTG CGAATGTTGC 1081 TGGTGATCGT TGTGCTTTTT TTTCTGTGTT GGTTGCCAGT TTATAGTGCC AACACGTGGC 1141 GCGCCTTTGA TGGCCCGGGT GCACACCGAG CACTCTCGGG TGCTCCTATC TCCTTCATTC 1201 ACTTGCTGAG CTACGCCTCG GCCTGTGTCA ACCCCCTGGT CTACTGCTTC ATGCACCGTC 1261 GCTTTCGCCA GGCCTGCCTG GAAACTTGCG CTCGCTGCTG CCCCCGGCCT CCACGAGCTC 1321 GCCCCAGGGC TCTTCCCGAT GAGGACCCTC CCACTCCCTC CATTGCTTCG CTGTCCAGGC 1381 TTAGCTACAC CACCATCAGC ACACTGGGCC CTGGCGACCC AGCTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 AGGGGCTCAA ACATGTTCAT GTGACACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt