Construct: ORF TRCN0000489880

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021385.1_s317c1
DNA Barcode:
TATCTAGATAGGGCTCACAGCACC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
SULF1 (23213)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489880
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23213 SULF1 sulfatase 1 NM_001128204.2 99.8% 100% 2613_2614insTAAGC
2 human 23213 SULF1 sulfatase 1 NM_001128205.2 99.8% 100% 2613_2614insTAAGC
3 human 23213 SULF1 sulfatase 1 NM_001128206.2 99.8% 100% 2613_2614insTAAGC
4 human 23213 SULF1 sulfatase 1 NM_015170.2 99.8% 100% 2613_2614insTAAGC
5 human 23213 SULF1 sulfatase 1 XM_024447112.1 99.8% 100% 2613_2614insTAAGC
6 human 23213 SULF1 sulfatase 1 XM_011517495.2 90.5% 86.9% 2284_2285ins143;2370_2371ins105
7 human 23213 SULF1 sulfatase 1 XM_017013250.2 90.5% 86.9% 2284_2285ins143;2370_2371ins105
8 human 23213 SULF1 sulfatase 1 XM_006716438.3 75.9% 76.2% 2550_2551ins34;2583T>G;2585_3366del
9 human 23213 SULF1 sulfatase 1 XM_006716439.3 75.9% 76.2% 2550_2551ins34;2583T>G;2585_3366del
10 human 23213 SULF1 sulfatase 1 XM_006716440.3 75.9% 76.2% 2550_2551ins34;2583T>G;2585_3366del
11 human 23213 SULF1 sulfatase 1 XM_011517494.2 75.9% 76.2% 2550_2551ins34;2583T>G;2585_3366del
12 human 23213 SULF1 sulfatase 1 XM_006716442.1 71.9% 71.8% (many diffs)
13 human 23213 SULF1 sulfatase 1 XM_006716441.1 54.5% 54.3% (many diffs)
14 human 23213 SULF1 sulfatase 1 NR_156414.2 44.9% (many diffs)
15 human 23213 SULF1 sulfatase 1 NR_156415.2 43.6% (many diffs)
16 human 23213 SULF1 sulfatase 1 NR_132437.1 37.6% (many diffs)
17 mouse 240725 Sulf1 sulfatase 1 NM_001198565.1 86% 92.8% (many diffs)
18 mouse 240725 Sulf1 sulfatase 1 NM_001198566.1 86% 92.8% (many diffs)
19 mouse 240725 Sulf1 sulfatase 1 NM_172294.1 86% 92.8% (many diffs)
20 mouse 240725 Sulf1 sulfatase 1 XM_006495510.3 65.5% 70.8% (many diffs)
21 mouse 240725 Sulf1 sulfatase 1 XM_006495511.3 65.5% 70.8% (many diffs)
22 mouse 240725 Sulf1 sulfatase 1 XM_006495512.3 65.5% 70.8% (many diffs)
23 mouse 240725 Sulf1 sulfatase 1 XM_006495513.3 65.5% 70.8% (many diffs)
24 mouse 240725 Sulf1 sulfatase 1 XM_006495514.1 65.5% 70.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2679
ORF length:
2613
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat
61tgaccatgaa gtattcttgc tgtgctctgg ttttggctgt cctgggcaca gaattgctgg
121gaagcctctg ttcgactgtc agatccccga ggttcagagg acggatacag caggaacgaa
181aaaacatccg acccaacatt attcttgtgc ttaccgatga tcaagatgtg gagctggggt
241ccctgcaagt catgaacaaa acgagaaaga ttatggaaca tgggggggcc accttcatca
301atgcctttgt gactacaccc atgtgctgcc cgtcacggtc ctccatgctc accgggaagt
361atgtgcacaa tcacaatgtc tacaccaaca acgagaactg ctcttccccc tcgtggcagg
421ccatgcatga gcctcggact tttgctgtat atcttaacaa cactggctac agaacagcct
481tttttggaaa atacctcaat gaatataatg gcagctacat cccccctggg tggcgagaat
541ggcttggatt aatcaagaat tctcgcttct ataattacac tgtttgtcgc aatggcatca
601aagaaaagca tggatttgat tatgcaaagg actacttcac agacttaatc actaacgaga
661gcattaatta cttcaaaatg tctaagagaa tgtatcccca taggcccgtt atgatggtga
721tcagccacgc tgcgccccac ggccccgagg actcagcccc acagttttct aaactgtacc
781ccaatgcttc ccaacacata actcctagtt ataactatgc accaaatatg gataaacact
841ggattatgca gtacacagga ccaatgctgc ccatccacat ggaatttaca aacattctac
901agcgcaaaag gctccagact ttgatgtcag tggatgattc tgtggagagg ctgtataaca
961tgctcgtgga gacgggggag ctggagaata cttacatcat ttacaccgcc gaccatggtt
1021accatattgg gcagtttgga ctggtcaagg ggaaatccat gccatatgac tttgatattc
1081gtgtgccttt ttttattcgt ggtccaagtg tagaaccagg atcaatagtc ccacagatcg
1141ttctcaacat tgacttggcc cccacgatcc tggatattgc tgggctcgac acacctcctg
1201atgtggacgg caagtctgtc ctcaaacttc tggacccaga aaagccaggt aacaggtttc
1261gaacaaacaa gaaggccaaa atttggcgtg atacattcct agtggaaaga ggcaaatttc
1321tacgtaagaa ggaagaatcc agcaagaata tccaacagtc aaatcacttg cccaaatatg
1381aacgggtcaa agaactatgc cagcaggcca ggtaccagac agcctgtgaa caaccggggc
1441agaagtggca atgcattgag gatacatctg gcaagcttcg aattcacaag tgtaaaggac
1501ccagtgacct gctcacagtc cggcagagca cgcggaacct ctacgctcgc ggcttccatg
1561acaaagacaa agagtgcagt tgtagggagt ctggttaccg tgccagcaga agccaaagaa
1621agagtcaacg gcaattcttg agaaaccagg ggactccaaa gtacaagccc agatttgtcc
1681atactcggca gacacgttcc ttgtccgtcg aatttgaagg tgaaatatat gacataaatc
1741tggaagaaga agaagaattg caagtgttgc aaccaagaaa cattgctaag cgtcatgatg
1801aaggccacaa ggggccaaga gatctccagg cttccagtgg tggcaacagg ggcaggatgc
1861tggcagatag cagcaacgcc gtgggcccac ctaccactgt ccgagtgaca cacaagtgtt
1921ttattcttcc caatgactct atccattgtg agagagaact gtaccaatcg gccagagcgt
1981ggaaggacca taaggcatac attgacaaag agattgaagc tctgcaagat aaaattaaga
2041atttaagaga agtgagagga catctgaaga gaaggaagcc tgaggaatgt agctgcagta
2101aacaaagcta ttacaataaa gagaaaggtg taaaaaagca agagaaatta aagagccatc
2161ttcacccatt caaggaggct gctcaggaag tagatagcaa actgcaactt ttcaaggaga
2221acaaccgtag gaggaagaag gagaggaaGG AGAAGAGACG GCAGAGGAAG GGGGAAGAGT
2281GCAGCCTGCC TGGCCTCACT TGCTTCACGC ATGACAACAA CCACTGGCAG ACAGCCCCGT
2341TCTGGAACCT GGGATCTTTC TGTGCTTGCA CGAGTTCTAA CAATAACACC TACTGGTGTT
2401TGCGTACAGT TAATGAGACG CATAATTTTC TTTTCTGTGA GTTTGCTACT GGCTTTTTGG
2461AGTATTTTGA TATGAATACA GATCCTTATC AGCTCACAAA TACAGTGCAC ACGGTAGAAC
2521GAGGCATTTT GAATCAGCTA CACGTACAAC TAATGGAGCT CAGAAGCTGT CAAGGATATA
2581AGCAGTGCAA CCCAAGACCT AAGAATCTTG ATGTTGGAAA TAAAGATGGA GGAAGCTATG
2641ACCTACACAG AGGACAGTTA TGGGATGGAT GGGAAGGTTA AGCTAGCGAC CCAGCTTTCT
2701TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2761CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2821GTGGAAAGGA CGATATCTAG ATAGGGCTCA CAGCACCACG CGTTAAGTCg acaatcaacc
2881tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)