Construct: ORF TRCN0000489907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020598.1_s317c1
- DNA Barcode:
- GGCAATTTCACTGAGGTCCCTCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ABL2 (27)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489907
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_005158.5 | 99.9% | 100% | 606G>A;1593C>T |
| 2 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | XM_005245088.2 | 98.1% | 98.2% | 109_110ins63;543G>A;1530C>T |
| 3 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_007314.4 | 97.1% | 95.6% | (many diffs) |
| 4 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_001168236.2 | 95.9% | 94.1% | (many diffs) |
| 5 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | XM_017001035.1 | 95.6% | 92.1% | (many diffs) |
| 6 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_001136000.3 | 91.1% | 91.1% | 606G>A;1593C>T;2013_2014ins309 |
| 7 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_001168239.2 | 89.3% | 89.3% | (many diffs) |
| 8 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_001168237.2 | 88.4% | 86.9% | (many diffs) |
| 9 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_001168238.2 | 87.2% | 85.4% | (many diffs) |
| 10 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | XR_921764.3 | 47.3% | (many diffs) | |
| 11 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | XR_921765.1 | 46.5% | (many diffs) | |
| 12 | human | 27 | ABL2 | ABL proto-oncogene 2, non-r... | NM_001136001.1 | 43.3% | 41% | (many diffs) |
| 13 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496614.3 | 89.6% | 93.5% | (many diffs) |
| 14 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496613.3 | 89.5% | 93.4% | (many diffs) |
| 15 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496617.3 | 87.8% | 91.7% | (many diffs) |
| 16 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_011238737.2 | 87.6% | 90.5% | (many diffs) |
| 17 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | NM_009595.3 | 87.5% | 90.5% | (many diffs) |
| 18 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496616.3 | 86.8% | 89% | (many diffs) |
| 19 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496615.3 | 86.7% | 88.9% | (many diffs) |
| 20 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496620.3 | 81.4% | 85.5% | (many diffs) |
| 21 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496622.3 | 79.6% | 83.7% | (many diffs) |
| 22 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_011238738.2 | 79.5% | 82.6% | (many diffs) |
| 23 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | NM_001136104.1 | 79.4% | 82.6% | (many diffs) |
| 24 | mouse | 11352 | Abl2 | v-abl Abelson murine leukem... | XM_006496621.3 | 78.7% | 81.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3573
- ORF length:
- 3501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcctt gggacagttc tccttccacc taatagttat ggcagagatc 121 aggacacttc actttgctgc ctgtgcactg aggcctcaga atctgctcta cccgacttaa 181 cagatcactt tgccagctgt gtggaggatg gatttgaggg agacaagact ggaggcagta 241 gtccagaagc tttgcatcgt ccctatggtt gtgatgttga accccaggca ctaaatgagg 301 ctatcaggtg gagctccaag gagaacttgc tcggagccac tgagagtgac cctaatctct 361 tcgttgcact ttatgatttt gtagcaagtg gtgataacac actcagcatc actaaaggtg 421 aaaagctacg agtccttggt tacaaccaga atggtgagtg gagtgaagtt cgctctaaga 481 atgggcaggg ctgggtgcca agcaactaca tcaccccagt gaacagcctg gaaaaacact 541 cctggtacca tggacctgtg tcacgcagtg cagctgagta tctgctcagc agtctaatca 601 atggcagctt cctggtgcga gaaagtgaga gtagccctgg gcagctgtcc atctcgctca 661 ggtacgaggg acgtgtatat cactacagga tcaataccac tgcagatggc aaggtgtatg 721 tgactgctga gagccgcttc agcaccttgg cagagcttgt acaccatcac tccacagtgg 781 ctgatgggct ggtgacaaca ttacactacc cagcacccaa gtgtaataag cctacagtct 841 atggtgtgtc ccccatccac gacaaatggg aaatggagcg aacagatatt accatgaagc 901 acaaacttgg gggcggtcag tatggagagg tttacgttgg cgtctggaag aaatacagcc 961 ttacagttgc tgtgaaaaca ttgaaggaag ataccatgga ggtagaagaa ttcctgaaag 1021 aagctgcagt aatgaaggaa atcaagcatc ctaatctggt acaactttta ggtgtgtgta 1081 ctttggagcc accattttac attgtgactg aatacatgcc atacgggaat ttgctggatt 1141 acctccgaga atgcaaccga gaagaggtga ctgcagttgt gctgctctac atggccactc 1201 agatttcttc tgcaatggag tacttagaga agaagaattt catccataga gatcttgcag 1261 ctcgtaactg cctagtggga gaaaaccatg tggtaaaagt ggctgacttt ggcttaagta 1321 gattgatgac tggagacact tatactgctc atgctggagc caaatttcct attaagtgga 1381 cagcaccaga gagtcttgcc tacaatacct tctcaattaa atctgacgtc tgggcttttg 1441 gggtattgtt gtgggaaatt gctacctatg gaatgtcacc atatccaggt attgacctgt 1501 ctcaggtcta tgacctacta gaaaaaggat atcgaatgga acagcctgag ggatgccccc 1561 ctaaggttta tgaacttatg agagcatgct ggaagtggag ccctgccgat aggccctctt 1621 ttgctgaaac acaccaagct tttgaaacca tgttccatga ctctagcatt tctgaagagg 1681 tagctgagga gcttgggaga gccgcctcct cgtcatctgt tgttccatac ctgccccggc 1741 tacctatact tccttccaag actcggacac tgaagaaaca ggtggagaac aaggagaaca 1801 ttgaaggggc acaagatgcc acagaaaatt ctgcttccag tttagcacca gggttcatca 1861 gaggtgcaca ggcctctagt ggatccccag cactgcctcg aaagcaaaga gacaagtcac 1921 ccagcagcct cttggaagat gccaaagaga catgcttcac cagggatagg aaggggggct 1981 tcttcagctc cttcatgaag aagagaaatg ctcctacacc ccccaaacgc agcagctcct 2041 tccgagaaat ggagaatcag ccccataaga aatacgaact cacgggtaac ttctcatctg 2101 ttgcttctct acagcatgct gatgggttct ctttcactcc tgcccagcaa gaggcgaatc 2161 tggtgccacc caagtgctat ggggggagct ttgcacagag gaacctctgt aatgacgacg 2221 gtggtggggg tgggggcagt ggcactgctg ggggtgggtg gtctggcatc acaggcttct 2281 ttacaccacg cttaatcaaa aagacactgg gcttacgagc aggtaaaccc acagccagtg 2341 atgacacttc caagcctttt ccaaggtcaa actctacatc ttccatgtcc tcagggcttc 2401 cagagcagga taggatggca atgacccttc ccaggaactg ccagaggtcc aaactccagc 2461 tggaaaggac agtgtccacc tcttctcagc cagaagagaa tgtggacagg gccaatgaca 2521 tgcttccaaa aaaatcagag gaaagtgctg ctccaagcag ggagagacca aaagccaagt 2581 tattgcccag aggagccaca gctcttcctc tcagaacacc ctctggggat ctagccatta 2641 cagagaagga ccctccaggg gtgggagtgg ctggagtggc agctgccccc aagggtaaag 2701 agaagaatgg tggggcacga cttgggatgg ctggagttcc agaggatgga gagcagccgg 2761 gctggccttc tccagccaag gctgcccccg tcctcccaac cactcacaac cacaaagtgc 2821 cagtccttat ctcacccact ctgaaacaca ctccagctga cgtgcagctc attggcacag 2881 actctcaggg gaataaattc aagctcttat ctgagcatca ggtcacatcc tctggagaca 2941 aggaccgacc ccgacgggta aaaccaaagt gtgccccacc cccaccacca gtgatgagac 3001 tactgcagca tccgtccatc tgctcagacc ctacagaaga gccaactgcc ctaactgcag 3061 gacagtccac atcagaaaca caggaaggag gaaagaaggc agctctgggc gcagtgccca 3121 tcagtgggaa agctgggagg ccagtgatgc ctccacctca agtgcctctg cccacaTCTT 3181 CCATCTCGCC AGCCAAAATG GCCAATGGCA CAGCAGGTAC TAAAGTGGCT CTGAGAAAAA 3241 CCAAACAGGC CGCTGAGAAA ATCTCAGCAG ACAAAATCAG CAAAGAGGCC CTGCTGGAAT 3301 GTGCTGACCT ACTGTCCAGT GCACTCACGG AACCTGTGCC CAACAGCCAG CTGGTAGACA 3361 CTGGACACCA GCTGCTTGAC TACTGCTCAG GCTATGTGGA CTGCATCCCT CAAACTCGCA 3421 ACAAATTTGC CTTCCGAGAG GCTGTGAGCA AACTGGAACT CAGCCTGCAG GAGCTACAGG 3481 TTTCTTCAGC AGCTGCTGGT GTGCCCGGGA CAAACCCTGT CCTTAATAAC TTATTGTCAT 3541 GTGTACAGGA AATCAGTGAT GTGGTGCAGA GGTAGGACCC AGCTTTCTTG TACAAAGTGG 3601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3721 AGGCAATTTC ACTGAGGTCC CTCATACGCG TTAAGTCgac aatcaacctc tggattacaa 3781 aatttgtgaa agatt