Transcript: Human NM_001136000.3

Homo sapiens ABL proto-oncogene 2, non-receptor tyrosine kinase (ABL2), transcript variant d, mRNA.

Source:
NCBI, updated 2019-08-02
Taxon:
Homo sapiens (human)
Gene:
ABL2 (27)
Length:
11615
CDS:
33..3227

Additional Resources:

NCBI RefSeq record:
NM_001136000.3
NBCI Gene record:
ABL2 (27)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146662 TGTACACCATCACTCCACAG pXPR_003 TGG 703 22% 5 1.067 ABL2 ABL2 77180
2 BRDN0001146737 TATCGAATGGAACAGCCTGA pXPR_003 GGG 1475 46% 9 0.893 ABL2 ABL2 77179
3 BRDN0001146627 GGTTCAACATCACAACCATA pXPR_003 GGG 195 6% 3 0.1285 ABL2 ABL2 77178
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001136000.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230772 TACAGTGGGTGCACGATATAT pLKO_005 5792 3UTR 100% 15.000 21.000 N ABL2 n/a
2 TRCN0000002029 CCAGGCACTAAATGAGGCTAT pLKO.1 245 CDS 100% 4.050 5.670 N ABL2 n/a
3 TRCN0000218815 GACAAACCCTGTCCTTAATAA pLKO_005 3161 CDS 100% 15.000 12.000 N ABL2 n/a
4 TRCN0000002031 GCGAACAGATATTACCATGAA pLKO.1 839 CDS 100% 4.950 3.960 N ABL2 n/a
5 TRCN0000002030 CCTCGTCATCTGTTGTTCCAT pLKO.1 1669 CDS 100% 3.000 2.400 N ABL2 n/a
6 TRCN0000195370 CCTATGGAATGTCACCATATC pLKO.1 1426 CDS 100% 1.080 0.864 N ABL2 n/a
7 TRCN0000230770 ATACATGCCATACGGGAATTT pLKO_005 1073 CDS 100% 13.200 9.240 N ABL2 n/a
8 TRCN0000195032 CCCTAAGGTTTATGAACTTAT pLKO.1 1520 CDS 100% 13.200 9.240 N ABL2 n/a
9 TRCN0000002033 CCCTCAAACTCGCAACAAATT pLKO.1 3059 CDS 100% 13.200 9.240 N ABL2 n/a
10 TRCN0000199920 GATGGGCTGGTGACAACATTA pLKO.1 744 CDS 100% 13.200 9.240 N ABL2 n/a
11 TRCN0000230771 TCCCTCAAACTCGCAACAAAT pLKO_005 3058 CDS 100% 13.200 9.240 N ABL2 n/a
12 TRCN0000199548 CCACTGAGAGTGACCCTAATC pLKO.1 298 CDS 100% 10.800 7.560 N ABL2 n/a
13 TRCN0000002032 CGGTCAGTATGGAGAGGTTTA pLKO.1 875 CDS 100% 10.800 7.560 N ABL2 n/a
14 TRCN0000199757 GTTCCATGACTCCAGCATTTC pLKO.1 1613 CDS 100% 10.800 7.560 N ABL2 n/a
15 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 8654 3UTR 100% 4.950 2.475 Y ERAP2 n/a
16 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 9333 3UTR 100% 4.050 2.025 Y P3H4 n/a
17 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 9333 3UTR 100% 4.050 2.025 Y ORAI2 n/a
18 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 9333 3UTR 100% 4.050 2.025 Y P3H4 n/a
19 TRCN0000138140 GATTGAGACCATCCTGGCTAA pLKO.1 8709 3UTR 100% 4.050 2.025 Y LOC441087 n/a
20 TRCN0000023348 GATGCCAAAGAGACATGCTTT pLKO.1 1899 CDS 100% 4.950 3.465 N Abl2 n/a
21 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 8655 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001136000.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489907 GGCAATTTCACTGAGGTCCCTCAT pLX_317 11.6% 91.1% 91.1% V5 (not translated due to prior stop codon) 606G>A;1593C>T;2013_2014ins309 n/a
2 ccsbBroadEn_14526 pDONR223 0% 89.3% 89.3% None 110_172del;2013_2014ins309 n/a
3 ccsbBroad304_14526 pLX_304 0% 89.3% 89.3% V5 110_172del;2013_2014ins309 n/a
4 TRCN0000471114 CACTAGGCCAACTGCGTGAGATAA pLX_317 10.1% 89.3% 89.3% V5 110_172del;2013_2014ins309;3192delG n/a
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