Construct: ORF TRCN0000489912

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019564.1_s317c1
DNA Barcode:
GGACAAGAAATCAACTTGTATCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP2CA (5515)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489912
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5515 PPP2CA protein phosphatase 2 catal... NM_002715.4 100% 100%
2 human 5516 PPP2CB protein phosphatase 2 catal... NM_001009552.2 83.1% 97.4% (many diffs)
3 human 5515 PPP2CA protein phosphatase 2 catal... NM_001355019.1 78.9% 78.9% 0_1ins195
4 human 5515 PPP2CA protein phosphatase 2 catal... NR_149151.1 33.2% (many diffs)
5 mouse 19052 Ppp2ca protein phosphatase 2 (form... NM_019411.4 94.2% 99.6% (many diffs)
6 mouse 19053 Ppp2cb protein phosphatase 2 (form... NM_017374.3 81.8% 97.4% (many diffs)
7 mouse 19053 Ppp2cb protein phosphatase 2 (form... XM_006509033.1 64.2% 78.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
1020
ORF length:
927
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg
61cggccgcccc cttcaccgga tccggtggca tcatggacga gaaggtgttc accaaggagc
121tggaccagtg gatcgagcag ctgaacgagt gcaagcagct gtccgagtcc caggtcaaga
181gcctctgcga gaaggctaaa gaaatcctga caaaagaatc caacgtgcaa gaggttcgat
241gtccagttac tgtctgtgga gatgtgcatg ggcaatttca tgatctcatg gaactgttta
301gaattggtgg caaatcacca gatacaaatt acttgtttat gggagattat gttgacagag
361gatattattc agttgaaaca gttacactgc ttgtagctct taaggttcgt taccgtgaac
421gcatcaccat tcttcgaggg aatcatgaga gcagacagat cacacaagtt tatggtttct
481atgatgaatg tttaagaaaa tatggaaatg caaatgtttg gaaatatttt acagatcttt
541ttgactatct tcctctcact gccttggtgg atgggcagat cttctgtcta catggtggtc
601tctcgccatc tatagataca ctggatcata tcagagcact tgatcgccTA CAAGAAGTTC
661CCCATGAGGG TCCAATGTGT GACTTGCTGT GGTCAGATCC AGATGACCGT GGTGGTTGGG
721GTATATCTCC TCGAGGAGCT GGTTACACCT TTGGGCAAGA TATTTCTGAG ACATTTAATC
781ATGCCAATGG CCTCACGTTG GTGTCTAGAG CTCACCAGCT AGTGATGGAG GGATATAACT
841GGTGCCATGA CCGGAATGTA GTAACGATTT TCAGTGCTCC AAACTATTGT TATCGTTGTG
901GTAACCAAGC TGCAATCATG GAACTTGACG ATACTCTAAA ATACTCTTTC TTGCAGTTTG
961ACCCAGCACC TCGTAGAGGC GAGCCACATG TTACTCGTCG TACCCCAGAC TACTTCCTGA
1021AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1081CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1141TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGACAAGAAA TCAACTTGTA
1201TCGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)