Construct: ORF TRCN0000489921

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021609.1_s317c1
DNA Barcode:
GGAAGCGGACCGCAAGTAAAATCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
NAT10 (55226)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489921
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55226 NAT10 N-acetyltransferase 10 NM_024662.3 99.9% 99.9% 1381T>C;1686C>T
2 human 55226 NAT10 N-acetyltransferase 10 NM_001144030.2 92.9% 92.8% 0_1ins216;1165T>C;1470C>T
3 mouse 98956 Nat10 N-acetyltransferase 10 NM_153126.2 88.8% 95.5% (many diffs)
4 mouse 98956 Nat10 N-acetyltransferase 10 XM_006500455.3 76.6% 80.7% (many diffs)
5 mouse 98956 Nat10 N-acetyltransferase 10 XR_374547.3 47.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3144
ORF length:
3075
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gcatcggaaa aaggtggata accgaatccg gattctcatt gagaatggag
121tagctgagcg gcaaagatct ctctttgttg tagttgggga tcgaggaaaa gatcaggtgg
181taatacttca tcacatgtta tccaaagcaa ctgtgaaggc tcggccttca gtgctgtggt
241gttataagaa agagctgggg tttagcagtc accggaagaa aagaatgcga cagctgcaga
301agaaaataaa gaatggaaca ctgaacataa agcaggacga cccctttgaa ctcttcatag
361cagccacaaa cattcgctac tgctactaca acgagaccca caagatcctg ggcaatacct
421tcggcatgtg tgtgctgcag gattttgaag ccttaactcc aaacttgctg gccaggactg
481tagaaacagt ggaaggtggt gggctagtgg tcatcctcct acggaccatg aactcactca
541agcaattgta cacagtgact atggatgtgc attccaggta cagaactgag gcccatcagg
601atgtggtggg aagatttaat gaaaggttta ttctgtctct ggcctcttgt aagaagtgtc
661tcgtcattga tgaccagctc aacatcctgc ccatctcctc ccacgttgcc accatggagg
721ccctgcctcc ccagactccg gatgagagtc ttggtccttc tgatctggag ctgagggagt
781tgaaggagag cttgcaggac acccagcctg tgggtgtgtt ggtggactgc tgtaagactc
841tagaccaggc caaagctgtc ttgaaattta tcgagggcat ctctgaaaag accctgagga
901gtactgttgc actcacagct gctcgaggac ggggaaaatc tgcagccctg ggattggcga
961ttgctggggc ggtggcattt gggtactcca atatctttgt tacctcccca agccctgata
1021acctccatac tctgtttgaa tttgtattta aaggatttga tgctctgcaa tatcaggaac
1081atctggatta tgagattatc cagtctctaa atcctgaatt taacaaagca gtgatcagag
1141tgaatgtatt tcgagaacac aggcagacta ttcagtatat acatcctgca gatgctgtga
1201agctgggcca ggctgaacta gttgtgattg atgaagctgc cgccatcccc ctccccttgg
1261tgaagagcct acttggcccc taccttgttt tcatggcatc caccatcaat ggctatgagg
1321gcactggccg gtcactgtcc ctcaagctaa ttcagcagct ccgtcaacag agcgcccaga
1381gccaggtcag caccactgct gagaataaga ccacgacgac agccagattg gcatcagcgc
1441ggacactgca tgaggtttcc ctccaggagt caatccgata cgcccctggg gatgcagtgg
1501agaagtggct gaatgacttg ctgtgcctgg attgcctcaa catcactcgg atagtctcag
1561gctgcccctt gcctgaagct tgtgaactgt actatgttaa tagagatacc ctcttttgct
1621accacaaggc ctctgaagtt ttcctccaac ggcttatggc cctctacgtg gcttctcact
1681acaagaactc tcccaatgat ctccagatgc tctccgatgc acctgctcac catctcttct
1741gccttctgcc tcctgtgccc cccacccaga atgcccttcc agaagtgctt gctgttatcc
1801aggtgtgcct tgaaggggag atttctcgcc agtccatctt gaacagtctg tctcgaggca
1861agaaggcttc aggggacctg attccatgga cagtgtcaga acagttccaa gatccagact
1921ttggtggtct gtctggtgga agggtcgttc gcattgctgt tcacccagat tatcaaggga
1981tgggctatgg cagccgtgct ctgcagctgc tgcagatgta ctatgaaggc aggtttcctt
2041gtctggagga aaaggtcctt gagacaccac aggaaattca caccgtaagc agcgaggctg
2101tcagcttgtt ggaagaggtc atcactcccc ggaaggacct gcctccttta ctcctcaaat
2161tgaatgagag gcctgccgaa cgcctggatt acctgggtgt ttcctatggc ttgaccccca
2221ggctcctcaa gttctggaaa cgagctggat ttgttcctgt ttatctgaga cagaccccga
2281atgacctgac cggagagcac tcgtgcatca tgctgaagac gctcactgat gaggatgagg
2341ctgaccaggg aggctggctt gcagccttct ggaaagattt ccgacggcgg ttcctagcct
2401tgctctccta ccagttcagt accttctctc cttccctggc tctgaacatc attcagaaca
2461ggaacatggg gaagccagcc cagcctgccc tgagccggga ggagctggaa gcactcttcc
2521tcccctatga cctgaagcgg ctggagatgt attcacggaa tatggtggac tatcacctca
2581tcatggacat gatcccggcc atctctcgca tctatttcct gaaccagctg ggggacctgg
2641ccctgtctgc ggctcagtcg gctcttctct tggggattgg cctGCAGCAT AAGTCTGTGG
2701ACCAGCTGGA AAAGGAGATT GAGCTGCCCT CGGGCCAGTT GATGGGACTT TTCAACCGGA
2761TCATCCGCAA AGTTGTGAAG CTATTTAATG AAGTTCAGGA AAAGGCCATT GAGGAGCAGA
2821TGGTGGCAGC GAAGGATGTG GTCATGGAGC CCACGATGAA GACCCTCAGT GACGACCTAG
2881ATGAAGCAGC AAAGGAATTT CAGGAGAAAC ACAAGAAGGA AGTAGGGAAG CTGAAGAGCA
2941TGGACCTCTC TGAATACATA ATCCGTGGGG ACGATGAAGA GTGGAATGAA GTTTTGAACA
3001AAGCTGGGCC GAACGCCTCG ATCATCAGCC TGAAAAGTGA CAAGAAAAGG AAGTTAGAGG
3061CCAAACAAGA ACCCAAACAG AGCAAGAAGT TGAAGAACAG AGAGACAAAG AACAAAAAAG
3121ATATGAAACT GAAGCGGAAG AAATAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
3181GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
3241AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGAAGCGG
3301ACCGCAAGTA AAATCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
3361aagatt

Download FASTA (ORF) (Full)