Construct: ORF TRCN0000491431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019089.2_s317c1
- DNA Barcode:
- CTCACCCCTTCATGGGAGAACTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FGFR2 (2263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_000141.4 | 96.2% | 95.3% | (many diffs) |
| 2 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001320658.2 | 95.9% | 95.1% | (many diffs) |
| 3 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_022970.3 | 93.3% | 92.2% | (many diffs) |
| 4 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_006717708.3 | 91.1% | 89.9% | (many diffs) |
| 5 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015921.2 | 89.9% | 97.2% | (many diffs) |
| 6 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_006717710.4 | 87.4% | 94.3% | (many diffs) |
| 7 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015920.2 | 87.2% | 94% | (many diffs) |
| 8 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144913.1 | 87.1% | 94% | (many diffs) |
| 9 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_023029.2 | 85.7% | 84.5% | (many diffs) |
| 10 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447887.1 | 83.8% | 82.6% | (many diffs) |
| 11 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447889.1 | 83.6% | 82.3% | (many diffs) |
| 12 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144914.1 | 83.2% | 81.9% | (many diffs) |
| 13 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144917.2 | 82.8% | 81.4% | (many diffs) |
| 14 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144916.1 | 82.7% | 81.2% | (many diffs) |
| 15 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144918.2 | 82.4% | 80.9% | (many diffs) |
| 16 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447890.1 | 81.2% | 79.5% | (many diffs) |
| 17 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144915.1 | 81.1% | 85.6% | (many diffs) |
| 18 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447888.1 | 80.9% | 79.3% | (many diffs) |
| 19 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015924.2 | 80.9% | 79.4% | (many diffs) |
| 20 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447891.1 | 78.2% | 76.3% | (many diffs) |
| 21 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144919.2 | 76.8% | 82.7% | (many diffs) |
| 22 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015925.2 | 76.7% | 82.8% | (many diffs) |
| 23 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001320654.1 | 68.5% | 64.8% | (many diffs) |
| 24 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447892.1 | 50.6% | 48% | 0_1ins1167;1129_1130ins92;1293_1294insG |
| 25 | human | 2263 | FGFR2 | fibroblast growth factor re... | NR_073009.2 | 49.9% | (many diffs) | |
| 26 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_010207.2 | 86.3% | 90.8% | (many diffs) |
| 27 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241674.2 | 86% | 90.5% | (many diffs) |
| 28 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507349.2 | 83.6% | 87.7% | (many diffs) |
| 29 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241673.2 | 83.6% | 87.7% | (many diffs) |
| 30 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321975.1 | 83.6% | 87.7% | (many diffs) |
| 31 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507350.3 | 83.3% | 87.5% | (many diffs) |
| 32 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321977.1 | 82.6% | 94.9% | (many diffs) |
| 33 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507352.2 | 80.2% | 91.9% | (many diffs) |
| 34 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321976.1 | 80% | 91.7% | (many diffs) |
| 35 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241675.2 | 76.8% | 80.9% | (many diffs) |
| 36 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507357.3 | 74.2% | 78.4% | (many diffs) |
| 37 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507354.3 | 74.2% | 77.9% | (many diffs) |
| 38 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_001347638.1 | 74% | 78.2% | (many diffs) |
| 39 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241676.2 | 73.9% | 77.6% | (many diffs) |
| 40 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_201601.2 | 71.7% | 75.4% | (many diffs) |
| 41 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241677.2 | 71.4% | 75.2% | (many diffs) |
| 42 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XR_001785478.1 | 34.5% | (many diffs) | |
| 43 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XR_001785477.1 | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2430
- ORF length:
- 2358
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcagc tggggtcgtt tcatctgcct ggtcgtggtc accatggcaa 121 ccttgtccct ggcccggccc tccttcagtt tagttgagga taccacatta gagccagaag 181 agccaccaac caaataccaa atctctcaac cagaagtgta cgtggctgcg ccaggggagt 241 cgctagaggt gcgctgcctg ttgaaagatg ccgccgtgat cagttggact aaggatgggg 301 tgcacttggg gcccaacaat aggacagtgc ttattgggga gtacttgcag ataaagggcg 361 ccacgcctag agactccggc ctctatgctt gtactgccag taggactgta gacagtgaaa 421 cttggtactt catggtgaat gtcacagatg ccatctcatc cggagatgat gaggatgaca 481 ccgatggtgc ggaagatttt gtcagtgaga acagtaacaa caagagagca ccatactgga 541 ccaacacaga aaagatggaa aagcggctcc atgctgtgcc tgcggccaac actgtcaagt 601 ttcgctgccc agccgggggg aacccaatgc caaccatgcg gtggctgaaa aacgggaagg 661 agtttaagca ggagcatcgc attggaggct acaaggtacg aaaccagcac tggagcctca 721 ttatggaaag tgtggtccca tctgacaagg gaaattatac ctgtgtggtg gagaatgaat 781 acgggtccat caatcacacg taccacctgg atgttgtgga gcgatcgcct caccggccca 841 tcctccaagc cggactgccg gcaaatgcct ccacagtggt cggaggagac gtagagtttg 901 tctgcaaggt ttacagtgat gcccagcccc acatccagtg gatcaagcac gtggaaaaga 961 acggcagtaa atacgggccc gacgggctgc cctacctcaa ggttctcaag gccggtgtta 1021 acaccacgga caaagagatt gaggttctct atattcggaa tgtaactttt gaggacgctg 1081 gggaatatac gtgcttggcg ggtaattcta ttgggatatc ctttcactct gcatggttga 1141 cagttctgcc agcgcctgga agagaaaagg agattacagc ttccccagac tacctggaga 1201 tagccattta ctgcataggg gtcttcttaa tcgcctgtat ggtggtaaca gtcatcctgt 1261 gccgaatgaa gaacacgacc aagaagccag acttcagcag ccagccggct gtgcacaagc 1321 tgaccaaacg tatccccctg cggagacagg taacagtttc ggctgagtcc agctcctcca 1381 tgaactccaa caccccgctg gtgaggataa caacacgcct ctcttcaacg gcagacaccc 1441 ccatgctggc aggggtctcc gagtatgaac ttccagagga cccaaaatgg gagtttccaa 1501 gagataagct gacactgggc aagcccctgg gagaaggttg ctttgggcaa gtggtcatgg 1561 cggaagcagt gggaattgac aaagacaagc ccaaggaggc ggtcaccgtg gccgtgaaga 1621 tgttgaaaga tgatgccaca gagaaagacc tttctgatct ggtgtcagag atggagatga 1681 tgaagatgat tgggaaacac aagaatatca taaatcttct tggagcctgc acacaggatg 1741 ggcctctcta tgtcatagtt gagtatgcct ctaaaggcaa cctccgagaa tacctccgag 1801 cccggaggcc acccgggatg gagtactcct atgacattaa ccgtgttcct gaggagcaga 1861 tgaccttcaa ggacttggtg tcatgcacct accagctggc cagaggcatg gagtacttgg 1921 cttcccaaaa atgtattcat cgagatttag cagccagaaa tgttttggta acagaaaaca 1981 atgtgatgaa aatagcagac tttggactcg ccagagatat caacaatata gactattaca 2041 aaaagaccac caatgggcgg cttccagtca agtggatggc tccagaagcc ctgtttgata 2101 gagtatacac tcatcagagt gatgtctggt ccttcggggt gttaatgtgg gagatcttca 2161 ctttaggggg ctcgccctac ccagggattc ccgtggagga actttttaag ctgctgaagg 2221 aaggacacag aatggataag ccagccaact gcaccaacga actgtacatg atgatgaggg 2281 actgttggca tgcagtgccc TCCCAGAGAC CAACGTTCAA GCAGTTGGTA GAAGACTTGG 2341 ATCGAATTCT CACTCTCACA ACCAATGAGG AATACTTGGA CCTCAGTCAG CCTCTCGAAC 2401 CGTATTCACC TTGTTATCCT GACCCAAGAT GAAATAAAAC GTCTCTCTTC CCTTCTTTCA 2461 GGAATACTTG GACCTCAGCC AACCTCTCGA ACAGTATTCA CCTAGTTACC CTGACACAAG 2521 AAGTTCTTGT TCTTCAGGAG ATGATTCTGT TTTTTCTCCA GACCCCATGC CTTACGAACC 2581 ATGCCTTCCT CAGTATCCAC ACATAAACGG CAGTGTTAAA ACAGACCCAG CTTTCTTGTA 2641 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 2701 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 2761 AAAGGACGAC TCACCCCTTC ATGGGAGAAC TCGACGCGTT AAGTCgacaa tcaacctctg 2821 gattacaaaa tttgtgaaag att