Construct: ORF TRCN0000491520

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020446.2_s317c1
DNA Barcode:
TTTCTTCGCCTCATCTCCCTTGTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
FGFR2 (2263)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491520
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2263 FGFR2 fibroblast growth factor re... NM_001320658.2 99.9% 100% 696A>G
2 human 2263 FGFR2 fibroblast growth factor re... NM_000141.4 99.7% 99.7% 696A>G;1282_1287delGTAACA
3 human 2263 FGFR2 fibroblast growth factor re... NM_022970.3 96.5% 96.4% (many diffs)
4 human 2263 FGFR2 fibroblast growth factor re... XM_006717708.3 94.5% 94.5% (many diffs)
5 human 2263 FGFR2 fibroblast growth factor re... XM_017015921.2 93.3% 93.3% (many diffs)
6 human 2263 FGFR2 fibroblast growth factor re... XM_017015920.2 90.3% 90.1% (many diffs)
7 human 2263 FGFR2 fibroblast growth factor re... XM_006717710.4 90% 89.9% (many diffs)
8 human 2263 FGFR2 fibroblast growth factor re... NM_001144913.1 90% 89.9% (many diffs)
9 human 2263 FGFR2 fibroblast growth factor re... NM_023029.2 88.8% 88.9% 107_108ins267;429A>G;1015_1020delGTAACA
10 human 2263 FGFR2 fibroblast growth factor re... XM_024447889.1 87% 87.1% 1_57del;164_165ins267;486A>G
11 human 2263 FGFR2 fibroblast growth factor re... XM_024447887.1 86.8% 86.9% (many diffs)
12 human 2263 FGFR2 fibroblast growth factor re... NM_001144914.1 86% 86.1% 696A>G;747_748ins336;946_951delGTAACA
13 human 2263 FGFR2 fibroblast growth factor re... NM_001144917.2 86% 86% 696A>G;937_938ins342
14 human 2263 FGFR2 fibroblast growth factor re... NM_001144918.2 85.9% 85.8% 109_110ins345;351A>G
15 human 2263 FGFR2 fibroblast growth factor re... NM_001144916.1 85.7% 85.6% 109_110ins345;351A>G;937_942delGTAACA
16 human 2263 FGFR2 fibroblast growth factor re... NM_001144915.1 84% 82.9% (many diffs)
17 human 2263 FGFR2 fibroblast growth factor re... XM_024447888.1 84% 83.9% (many diffs)
18 human 2263 FGFR2 fibroblast growth factor re... XM_024447890.1 83.8% 83.7% (many diffs)
19 human 2263 FGFR2 fibroblast growth factor re... XM_017015924.2 83.7% 83.6% (many diffs)
20 human 2263 FGFR2 fibroblast growth factor re... XM_024447891.1 80.7% 80.5% (many diffs)
21 human 2263 FGFR2 fibroblast growth factor re... XM_017015925.2 79.6% 79.4% (many diffs)
22 human 2263 FGFR2 fibroblast growth factor re... NM_001144919.2 79.3% 79.1% (many diffs)
23 human 2263 FGFR2 fibroblast growth factor re... NM_001320654.1 71% 69.1% (many diffs)
24 human 2263 FGFR2 fibroblast growth factor re... XM_024447892.1 52.2% 52.2% 0_1ins1170;112_117delGTAACA
25 human 2263 FGFR2 fibroblast growth factor re... NR_073009.2 50.9% (many diffs)
26 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241674.2 89.5% 95.3% (many diffs)
27 mouse 14183 Fgfr2 fibroblast growth factor re... NM_010207.2 89.3% 95.1% (many diffs)
28 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507350.3 86.5% 92.1% (many diffs)
29 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507349.2 86.3% 91.9% (many diffs)
30 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241673.2 86.3% 91.9% (many diffs)
31 mouse 14183 Fgfr2 fibroblast growth factor re... XM_017321975.1 86.3% 91.9% (many diffs)
32 mouse 14183 Fgfr2 fibroblast growth factor re... XM_017321977.1 85.8% 91% (many diffs)
33 mouse 14183 Fgfr2 fibroblast growth factor re... XM_017321976.1 82.8% 87.8% (many diffs)
34 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507352.2 82.6% 87.6% (many diffs)
35 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241675.2 79.5% 85.2% (many diffs)
36 mouse 14183 Fgfr2 fibroblast growth factor re... NM_001347638.1 77% 82.9% (many diffs)
37 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507357.3 76.9% 82.7% (many diffs)
38 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241676.2 76.7% 82.2% (many diffs)
39 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507354.3 76.5% 82% (many diffs)
40 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241677.2 74.1% 79.7% (many diffs)
41 mouse 14183 Fgfr2 fibroblast growth factor re... NM_201601.2 74% 79.5% (many diffs)
42 mouse 14183 Fgfr2 fibroblast growth factor re... XR_001785477.1 33.8% (many diffs)
43 mouse 14183 Fgfr2 fibroblast growth factor re... XR_001785478.1 33.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2529
ORF length:
2457
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtcagc tggggtcgtt tcatctgcct ggtcgtggtc accatggcaa
121ccttgtccct ggcccggccc tccttcagtt tagttgagga taccacatta gagccagaag
181agccaccaac caaataccaa atctctcaac cagaagtgta cgtggctgcg ccaggggagt
241cgctagaggt gcgctgcctg ttgaaagatg ccgccgtgat cagttggact aaggatgggg
301tgcacttggg gcccaacaat aggacagtgc ttattgggga gtacttgcag ataaagggcg
361ccacgcctag agactccggc ctctatgctt gtactgccag taggactgta gacagtgaaa
421cttggtactt catggtgaat gtcacagatg ccatctcatc cggagatgat gaggatgaca
481ccgatggtgc ggaagatttt gtcagtgaga acagtaacaa caagagagca ccatactgga
541ccaacacaga aaagatggaa aagcggctcc atgctgtgcc tgcggccaac actgtcaagt
601ttcgctgccc agccgggggg aacccaatgc caaccatgcg gtggctgaaa aacgggaagg
661agtttaagca ggagcatcgc attggaggct acaaggtacg aaaccagcac tggagcctca
721ttatggaaag tgtggtccca tctgacaagg gaaattatac ctgtgtggtg gagaatgaat
781acgggtccat caatcacacg taccacctgg atgttgtgga gcgatcgcct caccggccca
841tcctccaagc cggactgccg gcaaatgcct ccacagtggt cggaggagac gtagagtttg
901tctgcaaggt ttacagtgat gcccagcccc acatccagtg gatcaagcac gtggaaaaga
961acggcagtaa atacgggccc gacgggctgc cctacctcaa ggttctcaag gccgccggtg
1021ttaacaccac ggacaaagag attgaggttc tctatattcg gaatgtaact tttgaggacg
1081ctggggaata tacgtgcttg gcgggtaatt ctattgggat atcctttcac tctgcatggt
1141tgacagttct gccagcgcct ggaagagaaa aggagattac agcttcccca gactacctgg
1201agatagccat ttactgcata ggggtcttct taatcgcctg tatggtggta acagtcatcc
1261tgtgccgaat gaagaacacg accaagaagc cagacttcag cagccagccg gctgtgcaca
1321agctgaccaa acgtatcccc ctgcggagac aggtttcggc tgagtccagc tcctccatga
1381actccaacac cccgctggtg aggataacaa cacgcctctc ttcaacggca gacaccccca
1441tgctggcagg ggtctccgag tatgaacttc cagaggaccc aaaatgggag tttccaagag
1501ataagctgac actgggcaag cccctgggag aaggttgctt tgggcaagtg gtcatggcgg
1561aagcagtggg aattgacaaa gacaagccca aggaggcggt caccgtggcc gtgaagatgt
1621tgaaagatga tgccacagag aaagaccttt ctgatctggt gtcagagatg gagatgatga
1681agatgattgg gaaacacaag aatatcataa atcttcttgg agcctgcaca caggatgggc
1741ctctctatgt catagttgag tatgcctcta aaggcaacct ccgagaatac ctccgagccc
1801ggaggccacc cgggatggag tactcctatg acattaaccg tgttcctgag gagcagatga
1861ccttcaagga cttggtgtca tgcacctacc agctggccag aggcatggag tacttggctt
1921cccaaaaatg tattcatcga gatttagcag ccagaaatgt tttggtaaca gaaaacaatg
1981tgatgaaaat agcagacttt ggactcgcca gagatatcaa caatatagac tattacaaaa
2041agaccaccaa tgggcggctt ccagtcaagt ggatggctcc agaagccctg tttgatagag
2101tatacactca tcagagtgat gtctggtcct tcggggtgtt aatgtgggag atcttcactt
2161tagggggctc gccctaccca gggattcccg tggaggaact ttttaagctg ctgaaggaag
2221gacacagaat ggataagcca gccaactgca ccaacgaact gtacatgatg atgagggact
2281gttggcatgc agtgccctcc cagagaccaa cgttcaagca gttggtagaa gacttggatc
2341gaatTCTCAC TCTCACAACC AATGAGGAAT ACTTGGACCT CAGCCAACCT CTCGAACAGT
2401ATTCACCTAG TTACCCTGAC ACAAGAAGTT CTTGTTCTTC AGGAGATGAT TCTGTTTTTT
2461CTCCAGACCC CATGCCTTAC GAACCATGCC TTCCTCAGTA TCCACACATA AACGGCAGTG
2521TTAAAACATA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
2581ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
2641CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT CTTCGCCTCA TCTCCCTTGT
2701CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)