Construct: ORF TRCN0000491583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020719.2_s317c1
- DNA Barcode:
- CCCTCTGATACCGCCGCCACCAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BRAF (673)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491583
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_004333.4:c.1799T>A | 100% | 100% | |
| 2 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_004333.6 | 99.9% | 99.8% | 1799T>A |
| 3 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001354609.2 | 99.5% | 99.2% | (many diffs) |
| 4 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374244.1 | 94.9% | 94.9% | 1175_1294del;1919T>A |
| 5 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | NM_001374258.1 | 94.5% | 94.2% | (many diffs) |
| 6 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XM_017012559.1 | 89.2% | 87.6% | (many diffs) |
| 7 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744857.1 | 48.1% | (many diffs) | |
| 8 | human | 673 | BRAF | B-Raf proto-oncogene, serin... | XR_001744858.1 | 22.1% | (many diffs) | |
| 9 | mouse | 109880 | Braf | Braf transforming gene | XM_006505359.3 | 82.4% | 86.5% | (many diffs) |
| 10 | mouse | 109880 | Braf | Braf transforming gene | XM_006505358.3 | 82.1% | 86.1% | (many diffs) |
| 11 | mouse | 109880 | Braf | Braf transforming gene | XM_011241139.2 | 81.8% | 85.8% | (many diffs) |
| 12 | mouse | 109880 | Braf | Braf transforming gene | NM_139294.5 | 80.9% | 84.8% | (many diffs) |
| 13 | mouse | 109880 | Braf | Braf transforming gene | XM_011241138.2 | 80.7% | 84.6% | (many diffs) |
| 14 | mouse | 109880 | Braf | Braf transforming gene | XM_011241136.2 | 80.6% | 84.5% | (many diffs) |
| 15 | mouse | 109880 | Braf | Braf transforming gene | XM_011241137.1 | 80% | 83.9% | (many diffs) |
| 16 | mouse | 109880 | Braf | Braf transforming gene | XM_006505355.3 | 78.4% | 82.3% | (many diffs) |
| 17 | mouse | 109880 | Braf | Braf transforming gene | XM_011241135.2 | 78.2% | 82% | (many diffs) |
| 18 | mouse | 109880 | Braf | Braf transforming gene | XM_006505353.3 | 77.4% | 81.2% | (many diffs) |
| 19 | mouse | 109880 | Braf | Braf transforming gene | XM_011241134.2 | 77.2% | 80.9% | (many diffs) |
| 20 | mouse | 109880 | Braf | Braf transforming gene | XM_011241140.2 | 56.8% | 59.8% | (many diffs) |
| 21 | mouse | 109880 | Braf | Braf transforming gene | XR_001785085.1 | 43.4% | (many diffs) | |
| 22 | mouse | 109880 | Braf | Braf transforming gene | XR_001785086.1 | 39.9% | (many diffs) | |
| 23 | mouse | 109880 | Braf | Braf transforming gene | XR_001785087.1 | 38.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2370
- ORF length:
- 2298
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggcg ctgagcggtg gcggtggtgg cggcgcggag ccgggccagg 121 ctctgttcaa cggggacatg gagcccgagg ccggcgccgg cgccggcgcc gcggcctctt 181 cggctgcgga ccctgccatt ccggaggagg tgtggaatat caaacaaatg attaagttga 241 cacaggaaca tatagaggcc ctattggaca aatttggtgg ggagcataat ccaccatcaa 301 tatatctgga ggcctatgaa gaatacacca gcaagctaga tgcactccaa caaagagaac 361 aacagttatt ggaatctctg gggaacggaa ctgatttttc tgtttctagc tctgcatcaa 421 tggataccgt tacatcttct tcctcttcta gcctttcagt gctaccttca tctctttcag 481 tttttcaaaa tcccacagat gtggcacgga gcaaccccaa gtcaccacaa aaacctatcg 541 ttagagtctt cctgcccaac aaacagagga cagtggtacc tgcaaggtgt ggagttacag 601 tccgagacag tctaaagaaa gcactgatga tgagaggtct aatcccagag tgctgtgctg 661 tttacagaat tcaggatgga gagaagaaac caattggttg ggacactgat atttcctggc 721 ttactggaga agaattgcat gtggaagtgt tggagaatgt tccacttaca acacacaact 781 ttgtacgaaa aacgtttttc accttagcat tttgtgactt ttgtcgaaag ctgcttttcc 841 agggtttccg ctgtcaaaca tgtggttata aatttcacca gcgttgtagt acagaagttc 901 cactgatgtg tgttaattat gaccaacttg atttgctgtt tgtctccaag ttctttgaac 961 accacccaat accacaggaa gaggcgtcct tagcagagac tgccctaaca tctggatcat 1021 ccccttccgc acccgcctcg gactctattg ggccccaaat tctcaccagt ccgtctcctt 1081 caaaatccat tccaattcca cagcccttcc gaccagcaga tgaagatcat cgaaatcaat 1141 ttgggcaacg agaccgatcc tcatcagctc ccaatgtgca tataaacaca atagaacctg 1201 tcaatattga tgacttgatt agagaccaag gatttcgtgg tgatggagga tcaaccacag 1261 gtttgtctgc taccccccct gcctcattac ctggctcact aactaacgtg aaagccttac 1321 agaaatctcc aggacctcag cgagaaagga agtcatcttc atcctcagaa gacaggaatc 1381 gaatgaaaac acttggtaga cgggactcga gtgatgattg ggagattcct gatgggcaga 1441 ttacagtggg acaaagaatt ggatctggat catttggaac agtctacaag ggaaagtggc 1501 atggtgatgt ggcagtgaaa atgttgaatg tgacagcacc tacacctcag cagttacaag 1561 ccttcaaaaa tgaagtagga gtactcagga aaacacgaca tgtgaatatc ctactcttca 1621 tgggctattc cacaaagcca caactggcta ttgttaccca gtggtgtgag ggctccagct 1681 tgtatcacca tctccatatc attgagacca aatttgagat gatcaaactt atagatattg 1741 cacgacagac tgcacagggc atggattact tacacgccaa gtcaatcatc cacagagacc 1801 tcaagagtaa taatatattt cttcatgaag acctcacagt aaaaataggt gattttggtc 1861 tagctacaga gaaatctcga tggagtgggt cccatcagtt tgaacagttg tctggatcca 1921 ttttgtggat ggcaccagaa gtcatcagaa tgcaagataa aaatccatac agcTTTCAGT 1981 CAGATGTATA TGCATTTGGA ATTGTTCTGT ATGAATTGAT GACTGGACAG TTACCTTATT 2041 CAAACATCAA CAACAGGGAC CAGATAATTT TTATGGTGGG ACGAGGATAC CTGTCTCCAG 2101 ATCTCAGTAA GGTACGGAGT AACTGTCCAA AAGCCATGAA GAGATTAATG GCAGAGTGCC 2161 TCAAAAAGAA AAGAGATGAG AGACCACTCT TTCCCCAAAT TCTCGCCTCT ATTGAGCTGC 2221 TGGCCCGCTC ATTGCCAAAA ATTCACCGCA GTGCATCAGA ACCCTCCTTG AATCGGGCTG 2281 GTTTCCAAAC AGAGGATTTT AGTCTATATG CTTGTGCTTC TCCAAAAACA CCCATCCAGG 2341 CAGGGGGATA TGGTGCGTTT CCTGTCCACT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 2401 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2461 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 2521 CTCTGATACC GCCGCCACCA GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2581 ttgtgaaaga tt