Construct: ORF TRCN0000491728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013801.2_s317c1
- Derived from:
- ccsbBroadEn_04249
- DNA Barcode:
- CTGAGATAATATTGGGAATCTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIAPH3 (81624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491728
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81624 | DIAPH3 | diaphanous related formin 3 | NM_030932.3 | 100% | 100% | |
| 2 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XM_006719876.1 | 91% | 90.7% | (many diffs) |
| 3 | human | 81624 | DIAPH3 | diaphanous related formin 3 | NM_001258370.2 | 79.7% | 76.9% | (many diffs) |
| 4 | human | 81624 | DIAPH3 | diaphanous related formin 3 | NM_001258369.1 | 76.3% | 76.3% | 1_789del |
| 5 | human | 81624 | DIAPH3 | diaphanous related formin 3 | NM_001258368.2 | 75.3% | 75.1% | (many diffs) |
| 6 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XM_011535258.2 | 74.3% | 74.3% | (many diffs) |
| 7 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XM_024449422.1 | 74% | 74% | (many diffs) |
| 8 | human | 81624 | DIAPH3 | diaphanous related formin 3 | NM_001258367.2 | 73.8% | 73.5% | (many diffs) |
| 9 | human | 81624 | DIAPH3 | diaphanous related formin 3 | NM_001258366.2 | 71.6% | 71.4% | (many diffs) |
| 10 | human | 81624 | DIAPH3 | diaphanous related formin 3 | NM_001042517.2 | 70.9% | 70.7% | (many diffs) |
| 11 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XR_001749694.1 | 66.4% | 1_951del;2426_2447del;3313_3314ins207 | |
| 12 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XM_011535263.2 | 41% | 39% | (many diffs) |
| 13 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XM_011535265.2 | 40% | 39% | (many diffs) |
| 14 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XM_017020789.1 | 39.3% | 38.6% | (many diffs) |
| 15 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XR_941672.1 | 38.8% | (many diffs) | |
| 16 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XR_002957480.1 | 36.2% | (many diffs) | |
| 17 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XR_002957477.1 | 31.6% | (many diffs) | |
| 18 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XR_002957479.1 | 31.5% | (many diffs) | |
| 19 | human | 81624 | DIAPH3 | diaphanous related formin 3 | XR_002957478.1 | 31.5% | (many diffs) | |
| 20 | mouse | 56419 | Diaph3 | diaphanous related formin 3 | XM_006519312.3 | 64.3% | 62.1% | (many diffs) |
| 21 | mouse | 56419 | Diaph3 | diaphanous related formin 3 | XM_011245123.2 | 63.4% | 62.3% | (many diffs) |
| 22 | mouse | 56419 | Diaph3 | diaphanous related formin 3 | NM_019670.1 | 62.8% | 61.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2613
- ORF length:
- 2547
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgaggagagg agcctttcct tattggccaa agccgtggat cccagacacc 121 ccaatatgat gacagatgtg gttaaacttc tctctgcggt atgcattgta ggggaagaaa 181 gcatccttga agaagtttta gaagctttaa cttcagctgg tgaagaaaaa aaaattgaca 241 gatttttttg tattgtggaa ggcctccggc acaattcagt tcaactgcaa gtagcttgta 301 tgcagctcat caatgccctg gttacatctc ctgatgattt ggatttcagg cttcacatca 361 gaaatgaatt tatgcgttgt ggattgaaag agatattgcc aaatttaaaa tgcattaaga 421 atgatggcct ggatatccaa cttaaagtct ttgatgagca taaagaagaa gatttgtttg 481 agttatccca tcgccttgaa gatattagag ctgaacttga tgaagcatat gatgtttaca 541 acatggtgtg gagcacagtt aaagaaacta gagcagaggg atattttatt tctattcttc 601 agcatctttt gctgattcga aatgattatt ttataaggca acaatacttc aaattaattg 661 atgagtgtgt atcccagatt gtattgcata gagatggaat ggatccagac ttcacatatc 721 gaaaaagact agatttagat ttaacccagt ttgtagacat ttgcatagat caagcaaaac 781 tagaagagtt tgaagagaaa gcatcagaac tttacaagaa atttgaaaaa gagtttaccg 841 accaccaaga aactcaggct gaattgcaga aaaaagaggc aaagattaat gagcttcaag 901 cagagctaca agcttttaag tctcagtttg gtgccttgcc agctgattgt aatattcctt 961 tgcctccctc taaagaaggt ggaactggcc actcagcact tcctcctccg cctccactgc 1021 cttctggtgg aggggtgccg cctccacctc ctcccccacc acctcctcca cttccaggaa 1081 tgcggatgcc attcagtggt cctgtgcctc caccacctcc cctgggattc cttggaggac 1141 aaaattctcc tcctctacca atcctgccat ttgggttgaa accaaagaaa gaatttaaac 1201 ctgaaatcag catgagaaga ttgaattggt taaagatcag acctcatgaa atgactgaaa 1261 actgtttctg gataaaagta aatgaaaata agtatgaaaa cgtggatttg ctttgtaaac 1321 ttgagaatac attttgttgc caacaaaaag agagaagaga agaggaagat attgaagaga 1381 agaaatcgat taagaaaaaa attaaagaac ttaagttttt agattctaaa attgcccaga 1441 acctttcaat cttcctgagc tcttttcggg tgccatatga ggaaatcaga atgatgatat 1501 tggaagtaga tgaaacacgg ttggcagagt ctatgattca gaacttaata aagcatcttc 1561 ctgatcaaga gcaattaaat tcattgtctc agttcaagag tgaatatagc aacttatgtg 1621 aacctgagca gtttgtggtt gtgatgagca atgtgaagag actacggcca cggctcagtg 1681 ctattctctt taagcttcag tttgaagagc aggtgaacaa catcaaacct gacatcatgg 1741 ctgtcagtac tgcctgcgaa gagataaaga agagcaaaag ctttagcaag ttgctggaac 1801 ttgtattgct aatgggaaac tacatgaatg ctggctcccg gaatgctcaa accttcggat 1861 ttaaccttag ctctctctgt aaactaaagg acacaaaatc agcagatcag aaaacaacgc 1921 tacttcattt cctggtagaa atatgtgaag agaagtaccc tgatatactg aattttgtgg 1981 atgatttgga acctttagac aaagctagta aagtctctgt agaaacgctg gaaaagaatt 2041 tgaggcagat gggaaggcag cttcaacagc ttgagaagga attggaaacc tttccccctc 2101 ctgaggactt gcatgacaag tttgtgacaa agatgtccag atttgttatc agtgcaaaag 2161 aacaatatga gacactttcg aagttacacg aaaacatgga aaagttatac cagagtataa 2221 taggatacta tgccattgat gtgaagaagg tgtctgtgga agactttctt actgacctga 2281 ataacttcag aaCCACATTC ATGCAAGCAA TAAAGGAGAA TATCAAAAAA AGAGAAGCAG 2341 AGGAAAAAGA AAAACGTGTC AGAATAGCTA AAGAATTAGC AGAGCGAGAA AGACTCGAAC 2401 GCCAACAAAA GAAAAAGCGT TTATTAGAAA TGAAGACTGA GGGTGATGAG ACAGGAGTGA 2461 TGGATAATCT GCTGGAGGCC TTGCAGTCCG GGGCTGCCTT CCGCGACAGA AGAAAAAGGA 2521 CACCGATGCC AAAAGATGTT CGGCAGAGTC TCAGTCCAAT GTCTCAGAGG CCTGTTCTGA 2581 AAGTTTGTAA CCATGGTAAT AAACCGTATT TATACCCAAC TTTCTTGTAC AAAGTGGTTG 2641 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2701 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 2761 GAGATAATAT TGGGAATCTT CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2821 ttgtgaaaga tt