Construct: ORF TRCN0000491746

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019775.2_s317c1
DNA Barcode:
CCAAATTATCATACTCATACACAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCMT1 (5110)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491746
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_001360452.1 99.5% 99.1% 358G>A;498C>A;681_682insG
2 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_001360456.1 98.9% 97.8% (many diffs)
3 human 5110 PCMT1 protein-L-isoaspartate (D-a... XM_024446451.1 93.7% 91.3% (many diffs)
4 human 5110 PCMT1 protein-L-isoaspartate (D-a... XM_011535868.2 93.2% 90% (many diffs)
5 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_001252053.1 79.3% 79% (many diffs)
6 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_005389.2 79.3% 79% (many diffs)
7 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_001252049.1 78.9% 78.1% (many diffs)
8 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_001252050.1 67% 66.7% (many diffs)
9 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_001252051.1 67% 66.4% (many diffs)
10 human 5110 PCMT1 protein-L-isoaspartate (D-a... NM_001252052.1 66.6% 65.9% (many diffs)
11 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_017313833.1 82.9% 87.7% (many diffs)
12 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_017313832.1 82.3% 86.5% (many diffs)
13 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_017313834.1 77.1% 82.4% (many diffs)
14 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_017313835.1 77.1% 82.4% (many diffs)
15 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_006512594.2 76.6% 81.3% (many diffs)
16 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_011243140.1 76.6% 81.3% (many diffs)
17 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_011243141.2 76.6% 81.3% (many diffs)
18 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... NM_008786.2 72% 76.5% (many diffs)
19 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... NM_001347228.1 71.6% 75.6% (many diffs)
20 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_017313837.1 64% 68.8% (many diffs)
21 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_006512595.3 63.5% 67.8% (many diffs)
22 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_017313836.1 63.5% 67.8% (many diffs)
23 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_006512589.3 59.1% 57.1% (many diffs)
24 mouse 18537 Pcmt1 protein-L-isoaspartate (D-a... XM_006512590.2 53.1% 56.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
756
ORF length:
684
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcctgg aaatccggcg gcgccagcca ctcggagcta atccacaatc
121tccgcaaaaa tggaatcatc aagacagata aagtatttga agtgatgctg gctacagacc
181gctcccacta tgcaaaatgt aacccataca tggattctcc acaatcaata ggtttccaag
241caacaatcag tgctccacac atgcatgcat atgcgctaga acttctattt gatcagttgc
301atgaaggagc taaagctctt gatgtaggat ctggaagtgg aatccttact gcatgttttg
361cacgtatggt tGGATGTACT GGAAAAGTCA TAGGAATTGA TCACATTAAA GAGCTAGTAG
421ATGACTCAAT AAATAATGTC AGGAAGGACG ATCCAACACT TCTGTCTTCA GGGAGAGTAC
481AGCTTGTTGT GGGGGATGGA AGAATGGGAT ATGCTGAAGA AGCCCCTTAT GATGCCATTC
541ATGTGGGAGC TGCAGCCCCT GTTGTACCAC AGGCGCTAAT AGATCAGTTA AAGCCCGGAG
601GAAGATTGAT ATTGCCTGTT GGTCCTGCAG GCGGAAACCA AATGTTGGAG CAGTATGACA
661AGCTACAAGA TGGCAGCATC AAAATGAAGC CTCTGATGGG GGTGATATAC GTGCCTTTAA
721CAGATAAAGA AAAGCAGTGG TCCAGGTGGA AGGACCCAGC TTTCTTGTAC AAAGTGGTTG
781ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
841GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC
901AAATTATCAT ACTCATACAC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat
961ttgtgaaaga tt

Download FASTA (ORF) (Full)