Construct: ORF TRCN0000491750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021591.2_s317c1
- DNA Barcode:
- CTCTCCTCCCGGCACGGAATACTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PEX6 (5190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5190 | PEX6 | peroxisomal biogenesis fact... | NM_000287.4 | 99.9% | 100% | 2364G>A |
| 2 | human | 5190 | PEX6 | peroxisomal biogenesis fact... | XM_011514661.2 | 97.1% | 97.1% | 1041_1042ins84;2280G>A |
| 3 | human | 5190 | PEX6 | peroxisomal biogenesis fact... | NM_001316313.2 | 90.9% | 91% | 617_618ins264;2100G>A |
| 4 | human | 5190 | PEX6 | peroxisomal biogenesis fact... | NR_133009.2 | 78% | (many diffs) | |
| 5 | human | 5190 | PEX6 | peroxisomal biogenesis fact... | XR_001743466.2 | 65% | (many diffs) | |
| 6 | mouse | 224824 | Pex6 | peroxisomal biogenesis fact... | NM_145488.1 | 84.6% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3009
- ORF length:
- 2940
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgctggct gtcttgcggg tcctggagcc ctttccgacc gagacacccc 121 cgttggcagt gctgctgcca cccgggggcc cgtggccggc ggcggagctg ggcctggtgc 181 tggccctgag gcctgcaggg gagagcccgg cagggccggc gctgctggtg gcagccctgg 241 aggggccgga cgcgggcacc gaagagcagg gtcccgggcc gccgcagcta ctggttagcc 301 gcgcgctgct gcggctcctg gcactgggct ccggggcctg ggtgcgggcg cgggcggtgc 361 ggcggccccc ggcgctaggt tgggcactgc ttggcacctc gctggggcct gggctcggac 421 cgcgagtcgg gccgctgctg gtgaggcgcg gagagaccct cccagtgccc ggaccgcggg 481 tgctggagac gcggccggcg ttgcaagggc tgctgggccc agggactcgg ctggctgtga 541 ctgagctccg cgggcgggcc agactgtgtc cagagtctgg ggacagcagt cggcccccac 601 ccccgcccgt ggtgtcctcc tttgcggttt ctggcacagt gcggcgactc cagggagttc 661 tgggagggac tggagattca ctaggggtga gccggagctg tctccgtggc cttggcctct 721 tccagggcga atgggtgtgg gtggcccagg ccagagagtc atcgaacact tcacagccgc 781 acttggctag ggtgcaggtc ctagaacctc gctgggacct ctctgataga ctgggacccg 841 gctctggacc gctgggagag cccctcgctg acggactggc gcttgtccct gccactttgg 901 cttttaatct tggctgtgac cccctggaaa tgggagagct cagaattcag aggtacttgg 961 aaggctccat cgcccctgaa gacaaaggaa gctgctcatt gctgcctggg cctccatttg 1021 ccagagagtt acacatcgaa attgtgtctt ctccccacta cagcactaat ggaaattatg 1081 acggtgttct ttaccggcac tttcagatac ccagggtagt ccaggaaggg gatgttctat 1141 gtgtgccaac aattgggcaa gtagagatcc tggaaggaag tccagagaaa ctgcccaggt 1201 ggcgggaaat gttttttaaa gtgaagaaaa cagttgggga agctccagat ggaccagcca 1261 gtgcctactt ggccgacacc acccatacct ccttgtacat ggtgggttct accctgagcc 1321 ctgttccatg gctcccttca gaggaatcca ctctctggag cagtttgtct cctccaggcc 1381 tggaggcctt ggtgtctgaa ctctgtgctg tcctgaagcc tcgcctccag ccagggggtg 1441 ccctgctgac aggaactagc agtgtccttc tacggggccc cccaggctgt gggaagacca 1501 cagtagttgc tgctgcctgt agtcaccttg ggctccactt actgaaggtg ccctgctcca 1561 gcctctgtgc agaaagtagt ggggctgtgg agacaaaact gcaggccatc ttctcccggg 1621 cccgccgttg ccggcctgca gtcctgttgc tcacagctgt ggaccttctg ggccgggacc 1681 gtgatgggct gggtgaggat gcccgtgtga tggctgtgct gcgtcacctc ctcctcaatg 1741 aggaccccct caacagctgc cctcccctca tggttgtggc caccacaagc cgggcccagg 1801 acctgcctgc tgatgtgcag acagcatttc ctcatgagct cgaggtgcct gctctgtcag 1861 aggggcagcg gctcagcatc ctgcgggccc tcactgccca ccttcccctg ggccaggagg 1921 tgaacttggc acagctagca cggcggtgtg caggctttgt ggtaggggat ctctatgccc 1981 ttctgaccca cagcagccgg gcagcctgca ccaggatcaa gaactcaggt ttggcaggtg 2041 gcttgactga ggaggatgag ggggagctgt gtgctgccgg ctttcctctc ctggctgagg 2101 actttgggca ggcactggag caactgcaga cagctcactc ccaggccgtt ggagccccca 2161 agatcccctc agtgtcctgg catgatgtgg gtgggctgca ggaggtgaag aaggagatcc 2221 tggagaccat tcagctcccc ctggagcacc ctgagctact gagcctgggc ctgagacgct 2281 caggccttct gctccatggg ccccctggca ccggcaagac ccttctggcc aaggcagtag 2341 ccactgagtg cagccttacc ttcctcagcg tgaaggggcc agagctcatt aacatgtatg 2401 tgggccaaag tgaggagaat gtgcgggaag tatttgccag ggccagggct gcagctccat 2461 gcattatctt ctttgatgaa ctggactctt tggccccaag ccgggggcga agtggagatt 2521 ctggaggagt gatggacagg gtggtgtctc agctccttgc cgagctagat gggctgcaca 2581 gcactcagga tgtgtttgtg attggagcca ccaacagacc agatctcctg gaccctgccc 2641 ttctgcggcc tggcagattt gacaagctgg tgtttgtggg ggcaaatgag gaccgggcct 2701 cccagcTACG CGTTCTAAGT GCCATCACAC GCAAATTCAA GCTAGAGCCA TCTGTGAGCC 2761 TGGTAAACGT GCTAGATTGC TGCCCTCCCC AGCTGACGGG CGCGGACCTC TACTCTCTCT 2821 GCTCTGATGC TATGACAGCT GCCCTCAAAC GCAGGGTTCA TGACCTGGAG GAAGGGCTGG 2881 AGCCAGGTAG CTCAGCACTG ATGCTCACCA TGGAGGACTT GCTGCAGGCT GCCGCCCGGC 2941 TGCAACCCTC AGTCAGTGAG CAGGAGCTGC TCCGGTACAA GCGCATCCAG CGCAAGTTTG 3001 CTGCCTGCTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3061 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3121 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTC TCCTCCCGGC ACGGAATACT 3181 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t