Construct: ORF TRCN0000491778

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019783.2_s317c1
DNA Barcode:
AGGCAAGCATAGGGACCATCCCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TLK2 (11011)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491778
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11011 TLK2 tousled like kinase 2 NM_006852.4 99.9% 99.8% 2250_2251insG
2 human 11011 TLK2 tousled like kinase 2 XM_017024044.1 99.9% 99.8% 2250_2251insG
3 human 11011 TLK2 tousled like kinase 2 XM_017024046.1 99.9% 99.8% 2250_2251insG
4 human 11011 TLK2 tousled like kinase 2 XM_017024047.1 99.9% 99.8% 2250_2251insG
5 human 11011 TLK2 tousled like kinase 2 NM_001284333.2 97.1% 97% 1122_1187del;2316_2317insG
6 human 11011 TLK2 tousled like kinase 2 XM_011524216.2 97.1% 97% 1122_1187del;2316_2317insG
7 human 11011 TLK2 tousled like kinase 2 XM_011524217.3 97.1% 97% 1122_1187del;2316_2317insG
8 human 11011 TLK2 tousled like kinase 2 XM_011524222.3 97.1% 97% 1122_1187del;2316_2317insG
9 human 11011 TLK2 tousled like kinase 2 XM_024450553.1 97.1% 97% 1122_1187del;2316_2317insG
10 human 11011 TLK2 tousled like kinase 2 XM_024450552.1 96.2% 96.1% 1_21del;1143_1208del;2337_2338insG
11 human 11011 TLK2 tousled like kinase 2 XM_011524218.2 95.8% 95.7% 627_628ins93;2157_2158insG
12 human 11011 TLK2 tousled like kinase 2 NM_001284363.1 95.6% 95.6% 266_267ins96;2154_2155insG
13 human 11011 TLK2 tousled like kinase 2 XM_017024051.2 95.6% 95.6% 266_267ins96;2154_2155insG
14 human 11011 TLK2 tousled like kinase 2 XM_017024052.2 95.6% 95.6% 266_267ins96;2154_2155insG
15 human 11011 TLK2 tousled like kinase 2 XM_017024053.2 95.6% 95.6% 266_267ins96;2154_2155insG
16 human 11011 TLK2 tousled like kinase 2 XM_011524215.2 93% 93% 627_628ins93;1029_1094del;2223_2224insG
17 human 11011 TLK2 tousled like kinase 2 XM_011524221.2 92.9% 92.8% 266_267ins96;1026_1091del;2220_2221insG
18 human 11011 TLK2 tousled like kinase 2 XM_017024048.2 92.9% 92.8% 266_267ins96;1026_1091del;2220_2221insG
19 human 11011 TLK2 tousled like kinase 2 XM_017024049.2 92.9% 92.8% 266_267ins96;1026_1091del;2220_2221insG
20 human 11011 TLK2 tousled like kinase 2 XM_017024054.2 91.5% 91.4% 530_531ins189;2061_2062insG
21 human 11011 TLK2 tousled like kinase 2 XM_017024045.1 90.1% 90% 1_138del;404_405ins96;2292_2293insG
22 human 11011 TLK2 tousled like kinase 2 XM_011524220.2 88.9% 88.8% 530_531ins189;933_998del;2127_2128insG
23 human 11011 TLK2 tousled like kinase 2 XM_011524223.3 88.9% 88.8% (many diffs)
24 human 11011 TLK2 tousled like kinase 2 XM_017024055.2 87.2% 87.2% 266_267ins96;434_435ins189;1965_1966insG
25 human 11011 TLK2 tousled like kinase 2 XM_017024050.1 86.2% 86.1% (many diffs)
26 human 11011 TLK2 tousled like kinase 2 XM_017024056.2 86% 83.9% 0_1ins172;50_51ins140;1938_1939insG
27 human 11011 TLK2 tousled like kinase 2 XM_017024057.1 86% 83.9% 0_1ins172;50_51ins140;1938_1939insG
28 human 11011 TLK2 tousled like kinase 2 XM_011524224.3 83.6% 81.5% (many diffs)
29 human 11011 TLK2 tousled like kinase 2 XM_011524225.3 83.6% 81.5% (many diffs)
30 human 11011 TLK2 tousled like kinase 2 XM_011524226.2 83.6% 81.5% (many diffs)
31 human 11011 TLK2 tousled like kinase 2 XM_011524227.2 83.6% 81.5% (many diffs)
32 human 11011 TLK2 tousled like kinase 2 XM_011524228.3 83.6% 81.5% (many diffs)
33 human 11011 TLK2 tousled like kinase 2 NM_001330418.2 80% 80% 0_1ins447;1803_1804insG
34 human 11011 TLK2 tousled like kinase 2 XM_017024059.1 80% 80% 0_1ins447;1803_1804insG
35 human 11011 TLK2 tousled like kinase 2 XM_011524229.2 77.8% 77.7% 0_1ins447;675_740del;1869_1870insG
36 human 11011 TLK2 tousled like kinase 2 XM_017024060.1 71.7% 71.6% 0_1ins447;83_84ins189;1614_1615insG
37 human 11011 TLK2 tousled like kinase 2 XM_011524231.1 63.8% 63.1% (many diffs)
38 human 101928451 LOC101928451 uncharacterized LOC101928451 XR_927955.2 42.3% (many diffs)
39 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001112705.2 93.5% 99.2% (many diffs)
40 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533312.3 93.5% 99.2% (many diffs)
41 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533313.3 93.5% 99.2% (many diffs)
42 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314550.1 91.1% 96.6% (many diffs)
43 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294331.1 89.3% 95% (many diffs)
44 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_011249014.2 89.3% 95% (many diffs)
45 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533314.3 87.3% 92.4% (many diffs)
46 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533315.3 87% 92.6% (many diffs)
47 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533316.3 86.5% 91.8% (many diffs)
48 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314552.1 85.4% 90.8% (many diffs)
49 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294334.1 85% 86.1% (many diffs)
50 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314551.1 83.3% 88.4% (many diffs)
51 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294329.1 80.4% 83.6% (many diffs)
52 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533320.3 80.4% 83.6% (many diffs)
53 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294330.1 77% 77.8% (many diffs)
54 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XR_001780005.1 27.6% (many diffs)
55 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XR_001780006.1 27.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2325
ORF length:
2253
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgatggaa gaattgcata gcctggaccc acgacggcag gaattattgg
121aggccaggtt tactggagta ggtgttagta agggaccact taatagtgag tcttccaacc
181agagcttgtg cagcgtcgga tccttgagtg ataaagaagt agagactccc gagaaaaagc
241agaatgacca gcgaaatcgg aaaagaaaag ctgaaccata tgaaactagc caagggaaag
301gcactcctag gggacataaa attagtgatt actttgagtt tgctggggga agcgcgccag
361gaaccagccc tggcagaagt gttccaccag ttgcacgatc ctcaccgcaa cattccttat
421ccaatccctt accgcgacga gtagaacagc ccctctatgg tttagatggc agtgctgcaa
481aggaggcaac ggaggagcag tctgctctgc caaccctcat gtcagtgatg ctagcaaaac
541ctcggcttga cacagagcag ctggcgcaaa ggggagctgg cctctgcttc acttttgttt
601cagctcagca aaacagtccc tcatctacgg gatctggcaa cacagagcat tcctgcagct
661cccaaaaaca gatctccatc cagcacagac agacccagtc cgacctcaca atagaaaaaa
721tatctgcact agaaaacagt aagaattctg acttagagaa gaaggaggga agaatagatg
781atttattaag agccaactgt gatttgagac ggcagattga tgaacagcaa aagatgctag
841agaaatacaa ggaacgatta aatagatgtg tgacaatgag caagaaactc cttatagaaa
901agtcaaaaca agagaagatg gcgtgtagag ataagagcat gcaagaccgc ttgagactgg
961gccactttac tactgtccga cacggagcct catttactga acagtggaca gatggttatg
1021cttttcagaa tcttatcaag caacaggaaa ggataaattc acagagggaa gagatagaaa
1081gacaacggaa aatgttagca aagcggaaac ctcctgccat gggtcaggcc cctcctgcaa
1141ccaatgagca gaaacagcgg aaaagcaaga ccaatggagc tgaaaatgaa acgttaacgt
1201tagcagaata ccatgaacaa gaagaaatct tcaaactcag attaggtcat cttaaaaagg
1261aggaagcaga gatccaggca gagctggaga gactagaaag ggttagaaat ctacatatca
1321gggaactaaa aaggatacat aatgaagata attcacaatt taaagatcat ccaacgctaa
1381atgacagata tttgttgtta catcttttgg gtagaggagg tttcagtgaa gtttacaagg
1441catttgatct aacagagcaa agatacgtag ctgtgaaaat tcaccagtta aataaaaact
1501ggagagatga gaaaaaggag aattaccaca agcatgcatg tagggaatac cggattcata
1561aagagctgga tcatcccaga atagttaagc tgtatgatta cttttcactg gatactgact
1621cgttttgtac agtattagaa tactgtgagg gaaatgatct ggacttctac ctgaaacagc
1681acaaattaat gtcggagaaa gaggcccggt ccattatcat gcagattgtg aatgctttaa
1741agtacttaaa tgaaataaaa cctcccatca tacactatga cctcaaacca ggtaatattc
1801ttttagtaaa tggtacagcg tgtggagaga taaaaattac agattttggt ctttcgaaga
1861tcatggatga tgatagctac aattcagtgg atggcatgga gctaacatca caaggtgctg
1921gtacttattg gtatttacca ccagagtgtt ttgtggttGG GAAAGAACCA CCAAAGATCT
1981CAAATAAAGT TGATGTGTGG TCGGTGGGTG TGATCTTCTA TCAGTGTCTT TATGGAAGGA
2041AGCCTTTTGG CCATAACCAG TCTCAGCAAG ACATCCTACA AGAGAATACG ATTCTTAAAG
2101CTACTGAAGT GCAGTTCCCG CCAAAGCCAG TAGTAACACC TGAAGCAAAG GCGTTTATTC
2161GACGATGCTT GGCCTACCGA AAGGAGGACC GCATTGATGT CCAGCAGCTG GCCTGTGATC
2221CCTACTTGTT GCCTCACATC CGAAAGTCAG TCTCTACAAG TAGCCCTGCT GGAGCTGCTA
2281TTGCATCAAC CTCTGGGGCG TCCAATAACA GTTCTTCTAA TGACCCAGCT TTCTTGTACA
2341AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
2401AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
2461AGGACGAAGG CAAGCATAGG GACCATCCCT TACGCGTTAA GTCgacaatc aacctctgga
2521ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)