Construct: ORF TRCN0000491865

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004455.1_s317c1
Derived from:
ccsbBroadEn_13699
DNA Barcode:
TCAAGTGTCGATCAACATATTAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPDYE2 (441273)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491865
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_017012016.2 75.9% 74.2% (many diffs)
2 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_017012017.2 71.3% 62.1% (many diffs)
3 human 441273 SPDYE2 speedy/RINGO cell cycle reg... NM_001031618.3 64% 63.9% 1_432del;860C>G
4 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... NM_001166339.1 64% 63.9% 1_432del;860C>G
5 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... XM_005250093.4 64% 63.9% 1_432del;860C>G
6 human 441273 SPDYE2 speedy/RINGO cell cycle reg... XM_017012223.1 64% 63.9% 1_432del;860C>G
7 human 729597 SPDYE6 speedy/RINGO cell cycle reg... NM_001146210.4 64% 63.9% 1_432del;708T>C;1010A>G
8 human 442590 SPDYE5 speedy/RINGO cell cycle reg... NM_001306141.1 62.9% 61.9% (many diffs)
9 human 285955 SPDYE1 speedy/RINGO cell cycle reg... NM_175064.2 62.7% 61.3% (many diffs)
10 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... XM_011515702.3 60.5% 58.9% (many diffs)
11 human 441273 SPDYE2 speedy/RINGO cell cycle reg... XM_011516237.3 60.5% 58.9% (many diffs)
12 human 100996746 SPDYE11 speedy/RINGO cell cycle reg... XM_024446630.1 60% 41.6% (many diffs)
13 human 100996746 SPDYE11 speedy/RINGO cell cycle reg... NM_001351349.1 59% 54.2% (many diffs)
14 human 102723849 SPDYE17 speedy/RINGO cell cycle reg... NM_001351351.1 59% 54.2% (many diffs)
15 human 100505767 SPDYE18 speedy/RINGO cell cycle reg... NM_001351348.1 57.7% 52.5% (many diffs)
16 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_005249719.3 56.4% 55.2% (many diffs)
17 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_017012015.1 56.4% 55.2% (many diffs)
18 human 102723555 SPDYE16 speedy/RINGO cell cycle reg... NM_001351597.1 55.5% 52.4% (many diffs)
19 human 102723555 SPDYE16 speedy/RINGO cell cycle reg... XM_017012887.2 55.5% 52.4% (many diffs)
20 human 388333 SPDYE4 speedy/RINGO cell cycle reg... NM_001128076.3 51% 44.2% (many diffs)
21 human 388333 SPDYE4 speedy/RINGO cell cycle reg... XM_005256633.4 51% 44.2% (many diffs)
22 human 441251 SPDYE7P speedy/RINGO cell cycle reg... NR_003666.2 49.9% (many diffs)
23 human 441272 SPDYE3 speedy/RINGO cell cycle reg... XM_011516234.3 32.3% 23.7% (many diffs)
24 human 441272 SPDYE3 speedy/RINGO cell cycle reg... XM_011516235.3 32.3% 23.7% (many diffs)
25 human 441272 SPDYE3 speedy/RINGO cell cycle reg... NM_001004351.5 31.4% 28.6% (many diffs)
26 human 641776 SPDYE14P speedy/RINGO cell cycle reg... NR_146072.1 22.6% (many diffs)
27 human 105180390 SPDYE13P speedy/RINGO cell cycle reg... NR_146073.1 22.6% (many diffs)
28 human 105180391 SPDYE15P speedy/RINGO cell cycle reg... NR_146074.1 22.6% (many diffs)
29 human 728524 SPDYE8P speedy/RINGO cell cycle reg... NR_003664.2 18.5% (many diffs)
30 human 643862 SPDYE10P speedy/RINGO cell cycle reg... NR_146079.1 18.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
843
ORF length:
774
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagtggtgg gacgaatctg aggagtcgtt ggaggaggag ccacggaagg
121tgctcgcccc tgagcctgag gagatctggg tggcggagat gctgtgtggc ctcaagatga
181agctgaagcg acggcgagtg tcgctcgtgc tccctgagca ccacgaggcc ttcaacaggc
241tgcttgagga tcctgtcatt aaaagattct tggcctggga caaagatctg agggtgtcgg
301acaagtatct cctggctatg gtcatagcgt atttcagccg ggccggcttc ccctcctggc
361aataccaacg cattcatttc ttcctggctc tctacctggc caatgacatg gaggaggacg
421acgaggactc caaacaaaac atcttccact tcctgtatag gaagaaccgc tctcgcatac
481ccttgctccG TAAGCGTTGG TTCCAGTTAG GCCATTCCAT GAACCCGAGG GCCAGGAAGA
541ACCGCTCTCG CATACCCTTG CTCCGTAAGC GTCGGTTCCA GTTATACCGT TCCACGAACC
601CGAGGGCCAG GAAGAACCGC TCTCGCATAC CCTTGCTCCG TAAGCGTCGG TTCCAGTTAT
661ACCGTTCCAT GAACTCGAGG GCCAGGAAGA ACCGCTCTCA GATAGTCCTG TTCCAGAAAC
721GACGGTTCCA CTTCTTCTGT TCCATGAGCT GCAGGGCTTG GGTTTCCCCA GAGGAGTTGG
781AGGAGATCCA GGCTTATGAC CCAGAGCACT GGGTGTGGGC GCGAGATCGC GCTCACCTTT
841CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
901TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
961GGCTTTATAT ATCTTGTGGA AAGGACGATC AAGTGTCGAT CAACATATTA AGACGCGTTA
1021AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)

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