Construct: ORF TRCN0000491918
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021785.2_s317c1
- DNA Barcode:
- GCGGCCTCTAACTACATTGATACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SHC1 (6464)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491918
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6464 | SHC1 | SHC adaptor protein 1 | NM_003029.5 | 93.6% | 0_1ins96 | |
| 2 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_024449083.1 | 93.6% | 0_1ins96 | |
| 3 | human | 6464 | SHC1 | SHC adaptor protein 1 | NM_001130041.2 | 93.4% | 0_1ins96;919_920insCAG | |
| 4 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_024449084.1 | 93.4% | 0_1ins96;919_920insCAG | |
| 5 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_017002083.1 | 89.9% | 0_1ins96;419_420ins54;865_866insCAG | |
| 6 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_011509897.1 | 88.5% | 0_1ins96;419_420ins54;597_623del | |
| 7 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_005245451.4 | 86% | (many diffs) | |
| 8 | human | 6464 | SHC1 | SHC adaptor protein 1 | NM_001202859.2 | 84.5% | 0_1ins231;784_785insCAG | |
| 9 | human | 6464 | SHC1 | SHC adaptor protein 1 | NM_001130040.2 | 81.1% | (many diffs) | |
| 10 | human | 6464 | SHC1 | SHC adaptor protein 1 | NM_183001.5 | 80.9% | (many diffs) | |
| 11 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_011509892.3 | 79.9% | (many diffs) | |
| 12 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_011509893.3 | 79.7% | (many diffs) | |
| 13 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_017002081.2 | 78.1% | (many diffs) | |
| 14 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_017002082.2 | 77.9% | (many diffs) | |
| 15 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_011509894.3 | 76.9% | (many diffs) | |
| 16 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_011509898.2 | 75.4% | (many diffs) | |
| 17 | human | 6464 | SHC1 | SHC adaptor protein 1 | XM_005245449.5 | 75.1% | (many diffs) | |
| 18 | mouse | 20416 | Shc1 | src homology 2 domain-conta... | NM_011368.5 | 84% | (many diffs) | |
| 19 | mouse | 20416 | Shc1 | src homology 2 domain-conta... | NM_001113331.2 | 75.7% | (many diffs) | |
| 20 | mouse | 20416 | Shc1 | src homology 2 domain-conta... | XM_006501212.3 | 73.4% | (many diffs) | |
| 21 | mouse | 20416 | Shc1 | src homology 2 domain-conta... | XM_006501213.2 | 73.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 77
- ORF length:
- 12
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccca 61 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg 121 cttttttata atgccaactt tgtacaaaaa agcaggcacc atgaacaagc tgagtggagg 181 cggcgggcgc aggactcggg tggaaggggg ccagcttggg ggcgaggagt ggacccgcca 241 cgggagcttt gtcaataagc ccacgcgggg ctggctgcat cccaacgaca aagtcatggg 301 acccggggtt tcctacttgg ttcggtacat gggttgtgtg gaggtcctcc agtcaatgcg 361 tgccctggac ttcaacaccc ggactcaggt caccagggag gccatcagtc tggtgtgtga 421 ggctgtgccg ggtgctaagg gggcgacaag gaggagaaag ccctgtagcc gcccgctcag 481 ctctatcctg gggaggagta acctgaaatt tgctggaatg ccaatcactc tcaccgtctc 541 caccagcagc ctcaacctca tggccgcaga ctgcaaacag atcatcgcca accaccacat 601 gcaatctatc tcatttgcat ccggcgggga tccggacaca gccgagtatg tcgcctatgt 661 tgccaaagac cctgtgaatc agagagcctg ccacattctg gagtgtcccg aagggcttgc 721 ccaggatgtc atcagcacca ttggccaggc cttcgagttg cgcttcaaac aatacctcag 781 gaacccaccc aaactggtca cccctcatga caggatggct ggctttgatg gctcagcatg 841 ggatgaggag gaggaagagc cacctgacca tcagtactat aatgacttcc cggggaagga 901 accccccttg gggggggtgg tagacatgag gcttcgggaa ggagccgctc caggggctgc 961 tcgacccact gcacccaatg cccagacccc cagccacttg ggagctacat tgcctgtagg 1021 acagcctgtt gggggagatc cagaagtccg caaacagatg ccacctccac caccctgtcc 1081 agcaggcaga gagctttttg atgatccctc ctatgtcaac gtccagaacc tagacaaggc 1141 ccggcaagca gtgggtggtg ctgggccccc caatcctgct atcaatggca gtgcaccccg 1201 ggacctgttt gacatgaagc ccttcgaaga tgctcttcgc gtgcctccac ctccccagtc 1261 ggtgtccatg gcTGAGCAGC TCCGAGGGGA GCCCTGGTTC CATGGGAAGC TGAGCCGGCG 1321 GGAGGCTGAG GCACTGCTGC AGCTCAATGG GGACTTCCTG GTACGGGAGA GCACGACCAC 1381 ACCTGGCCAG TATGTGCTCA CTGGCTTGCA GAGTGGGCAG CCTAAGCATT TGCTACTGGT 1441 GGACCCTGAG GGTGTGGTTC GGACTAAGGA TCACCGCTTT GAAAGTGTCA GTCACCTTAT 1501 CAGCTACCAC ATGGACAATC ACTTGCCCAT CATCTCTGCG GGCAGCGAAC TGTGTCTACA 1561 GCAACCTGTG GAGCGGAAAC TGTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG 1621 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 1681 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCGGCCTCT 1741 AACTACATTG ATACCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 1801 agatt