Construct: ORF TRCN0000491954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020294.2_s317c1
- DNA Barcode:
- CGATCTATCACAATTATAACCTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NME1 (4830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 99.7% | 100% | 456_457insT |
| 2 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 85.7% | 85.8% | 1_75del;531_532insT |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 81.2% | 88.1% | (many diffs) |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 81.2% | 88.1% | (many diffs) |
| 5 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 81.2% | 88.1% | (many diffs) |
| 6 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 81.2% | 88.1% | (many diffs) |
| 7 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 53.7% | 50.1% | (many diffs) |
| 8 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 33.9% | (many diffs) | |
| 9 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | NM_008704.2 | 87.5% | 94% | (many diffs) |
| 10 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | XM_030245646.1 | 80.1% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 528
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaac tgtgagcgta ccttcattgc gatcaaacca gatggggtcc 121 agcggggtct tgtgggagag attatcaagc gttttgagca gaaaggatTC CGCCTTGTTG 181 GTCTGAAATT CATGCAAGCT TCCGAAGATC TTCTCAAGGA ACACTACGTT GACCTGAAGG 241 ACCGTCCATT CTTTGCCGGC CTGGTGAAAT ACATGCACTC AGGGCCGGTA GTTGCCATGG 301 TCTGGGAGGG GCTGAATGTG GTGAAGACGG GCCGAGTCAT GCTCGGGGAG ACCAACCCTG 361 CAGACTCCAA GCCTGGGACC ATCCGTGGAG ACTTCTGCAT ACAAGTTGGC AGGAACATTA 421 TACATGGCAG TGATTCTGTG GAGAGTGCAG AGAAGGAGAT CGGCTTGTGG TTTCACCCTG 481 AGGAACTGGT AGATTACACG AGCTGTGCTC AGAACTGGAT CTATGAATAG GACCCAGCTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGACGAT CTATCACAAT TATAACCTTA ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt