Construct: ORF TRCN0000492045

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014850.1_s317c1
Derived from:
ccsbBroadEn_07005
DNA Barcode:
ACAGCCTGGCCCTACACCTATTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DENND2B (6764)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492045
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6764 DENND2B DENN domain containing 2B NM_005418.3 99.8% 99.6% (many diffs)
2 human 6764 DENND2B DENN domain containing 2B NM_213618.2 99.8% 99.6% (many diffs)
3 human 6764 DENND2B DENN domain containing 2B XM_011520318.1 99.8% 99.6% (many diffs)
4 human 6764 DENND2B DENN domain containing 2B XM_005253077.1 97.3% 97% (many diffs)
5 human 6764 DENND2B DENN domain containing 2B XM_011520309.1 95.6% 95.4% (many diffs)
6 human 6764 DENND2B DENN domain containing 2B XM_017018186.1 84% 83.9% (many diffs)
7 human 6764 DENND2B DENN domain containing 2B XR_001747943.1 79.1% (many diffs)
8 human 6764 DENND2B DENN domain containing 2B XR_001747942.1 78.1% (many diffs)
9 human 6764 DENND2B DENN domain containing 2B XR_001747941.1 78% (many diffs)
10 human 6764 DENND2B DENN domain containing 2B XM_011520320.1 77.5% 76.4% (many diffs)
11 human 6764 DENND2B DENN domain containing 2B XM_011520322.1 75.6% 73.9% (many diffs)
12 human 6764 DENND2B DENN domain containing 2B XR_930887.2 75.4% (many diffs)
13 human 6764 DENND2B DENN domain containing 2B XM_011520321.2 74.3% 72.7% (many diffs)
14 human 6764 DENND2B DENN domain containing 2B XR_002957161.1 66.3% (many diffs)
15 human 6764 DENND2B DENN domain containing 2B XR_002957159.1 65.5% (many diffs)
16 human 6764 DENND2B DENN domain containing 2B XR_002957178.1 65.2% (many diffs)
17 human 6764 DENND2B DENN domain containing 2B XR_002957179.1 65.1% (many diffs)
18 human 6764 DENND2B DENN domain containing 2B XR_002957175.1 64.7% (many diffs)
19 human 6764 DENND2B DENN domain containing 2B XR_002957165.1 64.5% (many diffs)
20 human 6764 DENND2B DENN domain containing 2B XR_002957177.1 64.4% (many diffs)
21 human 6764 DENND2B DENN domain containing 2B XR_002957172.1 64.4% (many diffs)
22 human 6764 DENND2B DENN domain containing 2B XR_002957169.1 64.3% (many diffs)
23 human 6764 DENND2B DENN domain containing 2B XR_002957174.1 64.2% (many diffs)
24 human 6764 DENND2B DENN domain containing 2B XM_011520324.2 64.2% 62% (many diffs)
25 human 6764 DENND2B DENN domain containing 2B XR_002957168.1 64.1% (many diffs)
26 human 6764 DENND2B DENN domain containing 2B XR_002957163.1 64% (many diffs)
27 human 6764 DENND2B DENN domain containing 2B XR_002957166.1 63.8% (many diffs)
28 human 6764 DENND2B DENN domain containing 2B XR_002957170.1 63.5% (many diffs)
29 human 6764 DENND2B DENN domain containing 2B XR_002957167.1 62.7% (many diffs)
30 human 6764 DENND2B DENN domain containing 2B XR_002957164.1 62.6% (many diffs)
31 human 6764 DENND2B DENN domain containing 2B NM_139157.2 62.3% 60.4% (many diffs)
32 human 6764 DENND2B DENN domain containing 2B XM_005253083.2 62.3% 60.4% (many diffs)
33 human 6764 DENND2B DENN domain containing 2B XM_011520329.1 62.3% 60.4% (many diffs)
34 human 6764 DENND2B DENN domain containing 2B XR_002957176.1 61.6% (many diffs)
35 human 6764 DENND2B DENN domain containing 2B XR_002957162.1 61.3% (many diffs)
36 human 6764 DENND2B DENN domain containing 2B XR_002957160.1 60.7% (many diffs)
37 human 6764 DENND2B DENN domain containing 2B XR_002957171.1 60.1% (many diffs)
38 human 6764 DENND2B DENN domain containing 2B XR_002957173.1 57% (many diffs)
39 human 6764 DENND2B DENN domain containing 2B XR_002957182.1 47.9% (many diffs)
40 human 6764 DENND2B DENN domain containing 2B XR_002957187.1 45.7% (many diffs)
41 human 6764 DENND2B DENN domain containing 2B XR_002957188.1 45.7% (many diffs)
42 human 6764 DENND2B DENN domain containing 2B XR_002957183.1 45.2% (many diffs)
43 human 6764 DENND2B DENN domain containing 2B XR_002957185.1 44.4% (many diffs)
44 human 6764 DENND2B DENN domain containing 2B XR_002957181.1 44.3% (many diffs)
45 human 6764 DENND2B DENN domain containing 2B XR_002957186.1 43.8% (many diffs)
46 human 6764 DENND2B DENN domain containing 2B XR_002957180.1 43.8% (many diffs)
47 human 6764 DENND2B DENN domain containing 2B XR_002957184.1 43.2% (many diffs)
48 mouse 76954 St5 suppression of tumorigenici... NM_001001326.1 89.6% 92.7% (many diffs)
49 mouse 76954 St5 suppression of tumorigenici... XM_006508320.3 89.6% 92.7% (many diffs)
50 mouse 76954 St5 suppression of tumorigenici... XM_011241940.2 87.7% 90.7% (many diffs)
51 mouse 76954 St5 suppression of tumorigenici... XM_011241941.2 87.7% 90.7% (many diffs)
52 mouse 76954 St5 suppression of tumorigenici... XM_011241942.2 87.7% 90.7% (many diffs)
53 mouse 76954 St5 suppression of tumorigenici... XM_011241943.2 87.7% 90.7% (many diffs)
54 mouse 76954 St5 suppression of tumorigenici... XM_011241944.2 87.7% 90.7% (many diffs)
55 mouse 76954 St5 suppression of tumorigenici... XM_011241945.2 87.7% 90.7% (many diffs)
56 mouse 76954 St5 suppression of tumorigenici... NR_037617.1 68.7% (many diffs)
57 mouse 76954 St5 suppression of tumorigenici... XR_001778009.1 68.7% (many diffs)
58 mouse 76954 St5 suppression of tumorigenici... XM_011241946.2 62.4% 62.9% (many diffs)
59 mouse 76954 St5 suppression of tumorigenici... XM_011241947.2 62.3% 62.4% (many diffs)
60 mouse 76954 St5 suppression of tumorigenici... NM_029811.2 57.5% 60.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3477
ORF length:
3411
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc catgactgcc aacaagaatt ccagcatcac ccacggagct ggtggcacta
121aagcccctcg ggggactctg agcaggtctc agtcagtctc tccacctcca gttctctccc
181caccaaggag tcccatctac ccgctcagtg atagtgaaac ctcagcctgc aggtacccca
241gccactccag ctcccgggtg ctcctcaagg accggcaccc cccagctcct tcaccccaga
301atcctcaaga tccctcccca gatacttccc cacccacctg tcccttcaag accgccagct
361tcggttattt ggacagaagc ccttcggcgt gcaagagaga cgcccaaaag gaaagtgtcc
421aaggcgcagc ccaggatgta gcaggggtcg ctgcctgcct cccccttgcc cagagcacgc
481cattcccggg gccagcagct ggcccccggg gcgtcttgct gacccgtacc ggtacccgcg
541cccacagcct gggcatccgg gagaagatat cagcatggga aggtcgccga gaggcgtcgc
601ccaggatgag catgtgtgga gagaagcggg agggctctgg gagcgagtgg gcggccagtg
661agggctgccc cagcctgggc tgtcccagcg tggtgccgtc cccctgcagc tctgaaaaga
721cctttgattt caagggcctc cggaggatga gcaggacctt ctccgagtgt tcctacccag
781agactgagga ggagggagag gcgctccctg tccgggactc tttctaccgg ctggagaaac
841ggctgggccg gagtgagccc agcgccttcc tcagggggca tggcagcagg aaggagagct
901cagcagtgct gagccggatc cagaaaattg aacaggtcct gaaggagcag ccgggccggg
961ggctccccca gctccccagc agctgctaca gcgtggaccg ggggaaaagg aacactggaa
1021ccttgggctc cttggaggag ccggcagggg gcgcgagtgt gagcgctggc agccgggcag
1081tcggagtggc tggtgttgcg ggggaggcgg gcccaccccc agagagggaa ggcagtggtt
1141ccactaagcc cgggacccct ggaaatagcc ctagctccca gcggctgcca tcgaagagtt
1201ccctcgatcc cgctgtgaac cctgtcccca aacccaagcg cacctttgaa tacgaggctg
1261acaagaaccc caagagtaag cccagtaatg gtctacctcc ttcacccaca cctgctgctc
1321cacctccctt gccctccacc ccagccccgc cagtcacccg gagacccaag aaggacatgc
1381gtggtcaccg caagtcccag agcagaaaat cctttgagtt tgaggatgca tccagtctcc
1441agtccctgta cccctcttct cccactgaga atggtactga gaaccaaccc aagtttggat
1501ccaaaagcac tttagaagaa aatgcctatg aagatattgt gggagatctg cccaaggaga
1561atccatatga ggatgtggac ttaaagagcc gaagagcagg acgaaaatcc cagcaactgt
1621ctgagaactc cttggactct ttgcacagga tgtggagtcc tcaggacagg aagtacaaca
1681gcccgcccac acagctttcc ctgaaaccca acagccagtc cctgcgcagt gggaactggt
1741cagaaaggaa gagccaccgg ctgccacgat tacccaagag gcacagccat gacgacatgc
1801tgctgctggc tcagctgagt ctgccgtcct caccctccag cctcaatgaa gacagcctca
1861gcaccaccag cgagctgctg tccagccgcc gggcccgccg cattcccaag cttgtccaaa
1921gaactaactc catctacaat gccaagagag gaaagaagag attaaaaaag ttgtctatgt
1981ccagcattga aacagcatca ctgagagatg aaaacagtga gagcgagagc gactctgatg
2041acaggttcaa agcccacaca cagcgcctgg tccacatcca gtcgatgctg aagcgcgccc
2101ccagctatcg cacgctggag ctggagctgc tggagtggca ggagcgggag ctttttgagt
2161actttgtggt ggtgtccctc aagaagaagc catcgcgaaa cacctacctc cccgaagtct
2221cctaccagtt tcccaagctg gaccgaccca ccaagcagat gcgagaggca gaggaaaggc
2281tcaaagccat tccccagttt tgcttccctg atgccaagga ctggcttcct gtgtcagagt
2341atagcagtga gaccttttct ttcatgctga ctggggaaga tggcggcaga cgctttggct
2401actgcaggcg cttactgcca agtgggaaag ggccccggtt gccagaggtg tactgtgtca
2461tcagccgcct tggctgcttc ggcttgtttt ccaaggtcct agatgaggtg gagcgccggc
2521gtgggatctc cgctgcattg gtctatcctt tcatgagaag tctcatggag tcgcccttcc
2581cagccccagg gaagaccatc aaagtgaaga cattcctgcc aggtgctggc aatgaggtgt
2641tagagctgcg gcggcccatg gactcaaggc tggagcacgt ggactttgag tgccttttta
2701cctgcctcag tgtgcgccag ctcatccgaa tctttgcctc actgctgctg gagcgccggg
2761tcatttttgt ggcagataag ctcagtaccc tctccagctg ctcccacgcg gtggtggcct
2821tgctctaccc cttctcctgg cagcacacct tcattcctgt cctcccggcc tccatgattg
2881acatcgtctg ctgtcccacc cccttcctgg ttggcctgct ctccagctcc ctccccaaac
2941tgaaggagct gcctgtggag gaggcgctga tggtgaatct gggatctgac cgattcatcc
3001gacagatgga cgacgaagac acgttgttac ctaggaagtt acaggcagct ctggagcagg
3061ctctggagag gaagaatgag ctgatctccc aggactctga cagcgactcc gacgatgaat
3121gtaataccct caatgggctg gtgtcggagg tgtttatccg gttctttgtg gagaccgttg
3181ggcactactc ccTCTTTCTG ACACAGAGTG AGAAGGGAGA GAGGGCCTTT CAGCGAGAGG
3241CCTTCCGCAA ATCTGTGGCC TCCAAAAGCA TCCGCCGCTT TCTTGAGGTT TTTATGGAGT
3301CTCAGATGTT TGCTGGCTTC ATCCAAGACA GGGAGCTAAG AAAGTGTCGG GCAAAGGGCC
3361TTTTTGAGCA GCGAGTGGAG CAGTACTTAG AAGAACTCCC AGACACTGAG CAGAGTGGAA
3421TGAATAAGTT TCTCCGAGGT TTGGGCAACA AAATGAAGTT TCTCCACAAG AAGAATTACC
3481CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
3541TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
3601ATATATCTTG TGGAAAGGAC GAACAGCCTG GCCCTACACC TATTGTACGC GTTAAGTCga
3661caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)