Construct: ORF TRCN0000492103

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021517.2_s317c1
DNA Barcode:
TGTGGATTTAGGAAACATGCTTAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
EYA4 (2070)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492103
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2070 EYA4 EYA transcriptional coactiv... NM_172103.3 99.9% 100% 714G>A
2 human 2070 EYA4 EYA transcriptional coactiv... NM_001370458.1 98.2% 98.2% (many diffs)
3 human 2070 EYA4 EYA transcriptional coactiv... NM_004100.5 96.3% 96.2% 209_277del;783G>A
4 human 2070 EYA4 EYA transcriptional coactiv... XM_017010370.2 95.6% 92.9% (many diffs)
5 human 2070 EYA4 EYA transcriptional coactiv... NM_001301013.1 95.4% 95.3% 209_277del;783G>A;967_984del
6 human 2070 EYA4 EYA transcriptional coactiv... XM_017010371.2 94.9% 92.1% (many diffs)
7 human 2070 EYA4 EYA transcriptional coactiv... NM_172105.3 94.7% 94.5% (many diffs)
8 human 2070 EYA4 EYA transcriptional coactiv... NM_001370459.1 93.9% 94% 206_207ins93;621G>A;805_822del
9 human 2070 EYA4 EYA transcriptional coactiv... XM_005266851.5 93.8% 93.6% (many diffs)
10 human 2070 EYA4 EYA transcriptional coactiv... NM_001301012.1 93.2% 93.1% (many diffs)
11 human 2070 EYA4 EYA transcriptional coactiv... XM_017010369.2 93.1% 90.4% (many diffs)
12 human 2070 EYA4 EYA transcriptional coactiv... XM_017010368.2 92.3% 89.5% (many diffs)
13 human 2070 EYA4 EYA transcriptional coactiv... XM_017010374.2 92.3% 92.2% (many diffs)
14 human 2070 EYA4 EYA transcriptional coactiv... XM_017010373.2 91.6% 88.9% (many diffs)
15 human 2070 EYA4 EYA transcriptional coactiv... XM_017010372.2 90.8% 88.1% (many diffs)
16 human 2070 EYA4 EYA transcriptional coactiv... XR_001743219.2 68.9% (many diffs)
17 human 2070 EYA4 EYA transcriptional coactiv... XR_001743220.2 19.4% (many diffs)
18 mouse 14051 Eya4 EYA transcriptional coactiv... NM_010167.5 90.6% 96.5% (many diffs)
19 mouse 14051 Eya4 EYA transcriptional coactiv... NM_001347372.1 89% 94.8% (many diffs)
20 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512523.3 89% 94.8% (many diffs)
21 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512524.3 89% 94.8% (many diffs)
22 mouse 14051 Eya4 EYA transcriptional coactiv... XM_017313799.1 87.4% 92.9% (many diffs)
23 mouse 14051 Eya4 EYA transcriptional coactiv... XM_011243112.2 87% 91.1% (many diffs)
24 mouse 14051 Eya4 EYA transcriptional coactiv... XM_017313798.1 87% 91.1% (many diffs)
25 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512526.3 86.8% 90.4% (many diffs)
26 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512527.3 86.5% 91.4% (many diffs)
27 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512528.3 84.9% 90.9% (many diffs)
28 mouse 14051 Eya4 EYA transcriptional coactiv... XM_011243115.1 84.9% 90.9% (many diffs)
29 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512529.3 84.3% 90.2% (many diffs)
30 mouse 14051 Eya4 EYA transcriptional coactiv... XM_006512530.3 69.3% 74.8% (many diffs)
31 mouse 14051 Eya4 EYA transcriptional coactiv... XR_001779457.1 54.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1917
ORF length:
1848
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggaagactcc caggatttaa atgaacaatc agtaaagaaa acgtgcacag
121aatcagatgt ttcacaatct cagaattcca ggtctatgga aatgcaggac ctagcaagtc
181ctcatactct tgttggaggt ggtgatactc caggtagctc caaactggaa aaatctaatc
241tcagcagcac atcagttact acaaatggga caggagtgtc tcttcttgca gtcaaaacag
301agcccttgaa cagcagtgaa accacagcca cgactggaga tggagcgctt gacactttta
361ctgggtcagt aattacaagt agtggctaca gccccagatc agcacatcag tattccccac
421agctgtatcc ttccaagccc tatccacaca ttctttctac accagcagct caaacaatgt
481ctgcctatgc aggccagact cagtattcgg ggatgcagca gccagccgtc tacacagcct
541actcacagac aggacagccc tacagcttgc ccacttacga tttgggtgtg atgttgccag
601ccatcaagac agagagtgga ctttcccaaa ctcagtcccc attacagagt ggctgcctca
661gttacagccc agggttctct accccacagc caggccagac accttattct taccaaatgc
721caggttctag ttttgcacca tcatctacta tttatgcaaa taattcagtt tccaattcaa
781caaatttcag tggttcacaa caggattatc catcctatac agcctttggc caaaaccagt
841atgcacagta ttattcagca tcaacgtatg gagcgtatat gacatcgaat aacacagccg
901atggcacacc ctcttcaacc tctacttatc agttgcagga atctctccca ggactgacta
961accaaccagg agagttcgat accatgcaga gtccctccac acccatcaaa gatcttgatg
1021agagaacctg taggagttct gggtcaaagt ccagaggaag aggccggaaa aataatccct
1081ccccgcctcc tgatagtgac ctggagcgtg tgtttgtctg ggatttggat gaaaccatca
1141ttgtttttca ctcactgctc accgggtctt atgcacagaa gtatggcaag gatcccccca
1201tggctgtaac ccttggactc cgcatggaag aaatgatttt taatcttgct gatactcatt
1261tgttttttaa tgatttagag gagtgtgatc aagttcatat agatgatgtt tcctctgatg
1321ataatgggca ggacttaagt acctacagtt ttgcaactga tggcttccat gcagctgcaa
1381gtagtgcaaa cctttgtttg ccaacaggtg taagaggagg ggttgactgg atgaggaagt
1441tggcttttcg ttacagaaga gtaaaagaat tatataacac ctacaagaac aacgttggag
1501gactccttgg ccctgccaag agggatgcct ggctacagtt aagggcagag attgaaggtc
1561tgacagattc ctGGCTAACA AATGCACTTA AGTCTTTATC AATTATTAGC ACTAGGAGTA
1621ACTGCATAAA TGTCTTGGTA ACGACAACTC AACTGATCCC AGCACTTGCG AAGGTTCTAC
1681TCTATAGTTT AGGAGGTGCT TTCCCCATTG AGAATATTTA CAGTGCAACT AAAATAGGAA
1741AAGAAAGTTG CTTTGAACGA ATAATGCAAA GGTTTGGCAG AAAAGTAGTG TATGTTGTAA
1801TTGGGGATGG TGTAGAAGAA GAACAGGCAG CAAAAAAGCA CAACATGCCC TTCTGGAGGA
1861TATCCAGTCA CTCAGACCTC CTGGCTCTCC ACCAAGCACT GGAATTAGAG TATTTGTAAG
1921ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1981GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2041TTTATATATC TTGTGGAAAG GACGATGTGG ATTTAGGAAA CATGCTTATA CGCGTTAAGT
2101Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)