Construct: ORF TRCN0000492142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002079.2_s317c1
- Derived from:
- ccsbBroadEn_01682
- DNA Barcode:
- ATGCTTGCTGGTTTATCGGAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR137B (7107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7107 | GPR137B | G protein-coupled receptor ... | NM_003272.4 | 100% | 100% | |
| 2 | human | 7107 | GPR137B | G protein-coupled receptor ... | XM_017002209.2 | 93.2% | 91.9% | (many diffs) |
| 3 | human | 7107 | GPR137B | G protein-coupled receptor ... | XM_017002210.2 | 89.2% | 89.2% | 837_838ins129 |
| 4 | human | 7107 | GPR137B | G protein-coupled receptor ... | XR_247039.4 | 17.5% | 1_94del;1186_1333del;1440_6819del | |
| 5 | mouse | 83924 | Gpr137b | G protein-coupled receptor ... | NM_031999.2 | 86.6% | 91.2% | (many diffs) |
| 6 | mouse | 83924 | Gpr137b | G protein-coupled receptor ... | XM_006516805.3 | 80.2% | 82% | (many diffs) |
| 7 | mouse | 664862 | Gpr137b-ps | G protein-coupled receptor ... | NR_003568.1 | 36% | (many diffs) | |
| 8 | mouse | 83924 | Gpr137b | G protein-coupled receptor ... | XR_382078.1 | 27.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcccgagcgt ccccggccgc gcggcagcgc ccccggcccg atggagaccc 121 cgccgtggga cccagcccgc aacgactcgc tgccgcccac gctgaccccg gccgtgcccc 181 cctacgtgaa gcttggcctc accgtcgtct acaccgtgtt ctacgcgctg ctcttcgtgt 241 tcatctacgt gcagctctgg ctggtgctgc gttaccgcca caagcggctc agctaccaga 301 gcgtcttcct ctttctctgc ctcttctggg cctccctgcg gaccgtcctc ttctccttct 361 acttcaaaga cttcgtggcg gccaattcgc tcagcccctt cgtcttctgg ctgctctact 421 gcttccctgt gtgcctgcag tttttcaccc tcacgctgat gaacttgtac ttcacgcagg 481 tgattttcaa agccaagtca aaatattctc cagaattact caaataccgg ttgcccctct 541 acctggcctc cctcttcatc agccttgttt tcctgttggt gaatttaacc tgtgctgtgc 601 tggtaaagac gggaaattgg gagaggaagg ttatcgtctc tgtgcgagtg gccattaatg 661 acacgctctt cgtgctgtgt gccgtctctc tctccatctg tctctacaaa atctctaaga 721 tgtccttagc caacatttac ttggagtcca agggctcctc cgtgtgtcaa gtgactgcca 781 tcggtgtcac cgtgatactg ctttacacct ctcgggcctg ctacaacctg ttcatcctgt 841 cattttctca gaacaagagc gtccattcct ttgattatga ctggtacaat gtatcagacc 901 aggcagattt gaagaatcag ctgggagatg ctggatacgt attatttgga gtggtgttat 961 ttgtttggga actcttacct accaccttag tcGTTTATTT CTTCCGAGTT AGAAATCCTA 1021 CAAAGGACCT TACCAACCCT GGAATGGTCC CCAGCCATGG ATTCAGTCCC AGATCTTATT 1081 TCTTTGACAA CCCTCGAAGA TATGACAGTG ATGATGACCT TGCCTGGAAC ATTGCCCCTC 1141 AGGGACTTCA GGGAGGTTTT GCTCCAGATT ACTATGATTG GGGACAACAA ACTAACAGCT 1201 TCCTGGCACA AGCAGGAACT TTGCAAGACT CAACTTTGGA TCCTGACAAA CCAAGCCTTG 1261 GGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGAAT GCTTGCTGGT TTATCGGAAT TGACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt