Transcript: Human XR_247039.4

PREDICTED: Homo sapiens G protein-coupled receptor 137B (GPR137B), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GPR137B (7107)
Length:
6819
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_247039.4
NBCI Gene record:
GPR137B (7107)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_247039.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000359629 CTACCACCTTAGTCGTTTATT pLKO_005 1008 3UTR 100% 15.000 21.000 N GPR137B n/a
2 TRCN0000141528 CCTACCACCTTAGTCGTTTAT pLKO.1 1007 3UTR 100% 13.200 18.480 N GPR137B n/a
3 TRCN0000141583 CCACATTGGTAGACTCCTAAA pLKO.1 1915 3UTR 100% 10.800 15.120 N GPR137B n/a
4 TRCN0000144076 CGTATTATTTGGAGTGGTGTT pLKO.1 967 3UTR 100% 4.050 5.670 N GPR137B n/a
5 TRCN0000359630 GAGATGCTGGATACGTATTAT pLKO_005 954 3UTR 100% 15.000 10.500 N GPR137B n/a
6 TRCN0000141482 CACGCTGATGAACTTGTACTT pLKO.1 481 3UTR 100% 4.950 3.465 N GPR137B n/a
7 TRCN0000145342 GTCGTTTATTTCTTCCGAGTT pLKO.1 1019 3UTR 100% 4.050 2.835 N GPR137B n/a
8 TRCN0000140290 GCAGGAACTTTGCAAGACTCA pLKO.1 1389 3UTR 100% 2.640 1.848 N GPR137B n/a
9 TRCN0000359563 ATTCAGTCCCAGATCTTATTT pLKO_005 1090 3UTR 100% 15.000 9.000 N GPR137B n/a
10 TRCN0000141186 CCTCTTCTCCTTCTACTTCAA pLKO.1 376 3UTR 100% 4.950 2.475 Y GPR137B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_247039.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01682 pDONR223 100% 17.5% None 1_94del;1186_1333del;1440_6819del n/a
2 ccsbBroad304_01682 pLX_304 0% 17.5% V5 1_94del;1186_1333del;1440_6819del n/a
3 TRCN0000492142 ATGCTTGCTGGTTTATCGGAATTG pLX_317 23% 17.5% V5 1_94del;1186_1333del;1440_6819del n/a
4 TRCN0000492027 TCCTTTATCAAACAGACGACCCCC pLX_317 27.8% 17.5% V5 1_94del;1186_1333del;1440_6819delinsG n/a
5 TRCN0000487904 CACACATTTATCAATTGGGTTTGA pLX_317 23.6% 17.5% V5 (not translated due to prior stop codon) 1_94del;1186_1333del;1440_6819del n/a
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