Transcript: Mouse NR_003568.1

Mus musculus G protein-coupled receptor 137B, pseudogene (Gpr137b-ps), non-coding RNA.

Source:
NCBI, updated 2017-04-30
Taxon:
Mus musculus (mouse)
Gene:
Gpr137b-ps (664862)
Length:
2956
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_003568.1
NBCI Gene record:
Gpr137b-ps (664862)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NR_003568.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243489 TCACCATACCTGCAATGATTT pLKO_005 2660 3UTR 100% 13.200 6.600 Y Gpr137b n/a
2 TRCN0000241828 CTCCTCTTCGTGTTCATCTAT pLKO_005 228 3UTR 100% 5.625 2.813 Y Gpr137b n/a
3 TRCN0000243491 CCCGTGTGTCTACAGTTCTTC pLKO_005 426 3UTR 100% 4.950 2.475 Y Gpr137b n/a
4 TRCN0000243490 CGCTCATGAACTTGTACTTCA pLKO_005 454 3UTR 100% 4.950 2.475 Y Gpr137b n/a
5 TRCN0000241829 CTTCACCCTCACGCTCATGAA pLKO_005 443 3UTR 100% 4.950 2.475 Y Gpr137b n/a
6 TRCN0000173595 GAGCTACTCAAATACCGGTTA pLKO.1 513 3UTR 100% 4.050 2.025 Y Gpr137b n/a
7 TRCN0000173873 GCATCAAGACTCCACTTTGGA pLKO.1 1223 3UTR 100% 3.000 1.500 Y Gpr137b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_003568.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01682 pDONR223 100% 36% None (many diffs) n/a
2 ccsbBroad304_01682 pLX_304 0% 36% V5 (many diffs) n/a
3 TRCN0000492142 ATGCTTGCTGGTTTATCGGAATTG pLX_317 23% 36% V5 (many diffs) n/a
4 TRCN0000492027 TCCTTTATCAAACAGACGACCCCC pLX_317 27.8% 36% V5 (many diffs) n/a
5 TRCN0000487904 CACACATTTATCAATTGGGTTTGA pLX_317 23.6% 36% V5 (not translated due to prior stop codon) (many diffs) n/a
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