Construct: ORF TRCN0000492210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014673.1_s317c1
- Derived from:
- ccsbBroadEn_07663
- DNA Barcode:
- CGTCCGTTCGGGGGCACGCAGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGF2BP3 (10643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492210
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10643 | IGF2BP3 | insulin like growth factor ... | NM_006547.3 | 99.9% | 100% | 1332G>A |
| 2 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_011515089.2 | 99% | 99.1% | 285_299del;1347G>A |
| 3 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_011515092.2 | 83.7% | 83.5% | (many diffs) |
| 4 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_011515093.2 | 83.7% | 83.5% | (many diffs) |
| 5 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_011515090.3 | 80% | 75.8% | (many diffs) |
| 6 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_006715639.2 | 72.8% | 72.8% | 0_1ins471;861G>A |
| 7 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_011515091.2 | 72.8% | 72.8% | 0_1ins471;861G>A |
| 8 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | NM_023670.3 | 91.8% | 96.5% | (many diffs) |
| 9 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | XM_006505517.3 | 84.7% | 87.1% | (many diffs) |
| 10 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | XR_377421.3 | 53.9% | (many diffs) | |
| 11 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | XM_006505519.3 | 50.7% | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caaactgtat atcggaaacc tcagcgagaa cgccgccccc tcggacctag 121 aaagtatctt caaggacgcc aagatcccgg tgtcgggacc cttcctggtg aagactggct 181 acgcgttcgt ggactgcccg gacgagagct gggccctcaa ggccatcgag gcgctttcag 241 gtaaaataga actgcacggg aaacccatag aagttgagca ctcggtccca aaaaggcaaa 301 ggattcggaa acttcagata cgaaatatcc cgcctcattt acagtgggag gtgctggata 361 gtttactagt ccagtatgga gtggtggaga gctgtgagca agtgaacact gactcggaaa 421 ctgcagttgt aaatgtaacc tattccagta aggaccaagc tagacaagca ctagacaaac 481 tgaatggatt tcagttagag aatttcacct tgaaagtagc ctatatccct gatgaaatgg 541 ccgcccagca aaaccccttg cagcagcccc gaggtcgccg ggggcttggg cagaggggct 601 cctcaaggca ggggtctcca ggatccgtat ccaagcagaa accatgtgat ttgcctctgc 661 gcctgctggt tcccacccaa tttgttggag ccatcatagg aaaagaaggt gccaccattc 721 ggaacatcac caaacagacc cagtctaaaa tcgatgtcca ccgtaaagaa aatgcggggg 781 ctgctgagaa gtcgattact atcctctcta ctcctgaagg cacctctgcg gcttgtaagt 841 ctattctgga gattatgcat aaggaagctc aagatataaa attcacagaa gagatcccct 901 tgaagatttt agctcataat aactttgttg gacgtcttat tggtaaagaa ggaagaaatc 961 ttaaaaaaat tgagcaagac acagacacta aaatcacgat atctccattg caggaattga 1021 cgctgtataa tccagaacgc actattacag ttaaaggcaa tgttgagaca tgtgccaaag 1081 ctgaggagga gatcatgaag aaaatcaggg agtcttatga aaatgatatt gcttctatga 1141 atcttcaagc acatttaatt cctggattaa atctgaacgc cttgggtctg ttcccaccca 1201 cttcagggat gccacctccc acctcagggc ccccttcagc catgactcct ccctacccgc 1261 agtttgagca atcagaaacg gagactgttc atctgtttat cccagctcta tcagtcggtg 1321 ccatcatcgg caagcagggc cagcacatca agcagctttc tcgctttgct GGAGCTTCAA 1381 TTAAGATTGC TCCAGCAGAA GCACCAGATG CTAAAGTGAG GATGGTGATT ATCACTGGAC 1441 CACCAGAGGC TCAGTTCAAG GCTCAGGGAA GAATTTATGG AAAAATTAAA GAAGAAAACT 1501 TTGTTAGTCC TAAAGAAGAG GTGAAACTTG AAGCTCATAT CAGAGTGCCA TCCTTTGCTG 1561 CTGGCAGAGT TATTGGAAAA GGAGGCAAAA CGGTGAATGA ACTTCAGAAT TTGTCAAGTG 1621 CAGAAGTTGT TGTCCCTCGT GACCAGACAC CTGATGAGAA TGACCAAGTG GTTGTCAAAA 1681 TAACTGGTCA CTTCTATGCT TGCCAGGTTG CCCAGAGAAA AATTCAGGAA ATTCTGACTC 1741 AGGTAAAGCA GCACCAACAA CAGAAGGCTC TGCAAAGTGG ACCACCTCAG TCAAGACGGA 1801 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGACG TCCGTTCGGG GGCACGCAGC CGACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt